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• W2756408218 abstract "Abstract Quickly and precisely gain genetically enhanced breeding elites with value-adding performance traits is desired by the crop breeders all the time. The present of gene editing technologies, especially the CRISPR/Cas9 system with the capacities of efficiency, versatility and multiplexing provides a reasonable expectation towards breeding goals. For exploiting possible application to accelerate the speed of process at breeding by CRISPR/Cas9 technology, in this study, the Agrobacterium tumefaciens -mediated CRISPR/Cas9 system transformation method was used for obtaining tomato ALC gene mutagenesis and replacement, in absence and presence of the homologous repair template. The average mutation frequency (72.73%) and low replacement efficiency (7.69%) were achieved in T 0 transgenic plants respectively. None of homozygous mutation was detected in T 0 transgenic plants, but one plant carry the heterozygous genes ( Cas9 / * - ALC / alc ) was stably transmitted to T 1 generations for segregation and genotyping. Finally, the desired alc homozygous mutants without T-DNA insertion (*/*- alc / alc ) in T 1 generations were acquired and further confirmed by genotype and phenotype characterization, with highlight of excellent storage performance, thus the recessive homozygous breeding elites with the character of long-shelf life were generated. Our results support that CRISPR/Cas9-induced gene replacement via HDR provides a valuable method for breeding elite innovation in tomato." @default.
• W2756408218 created "2017-09-25" @default.
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• W2756408218 date "2017-09-19" @default.
• W2756408218 modified "2023-10-10" @default.
• W2756408218 title "CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines" @default.
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• W2756408218 doi "https://doi.org/10.1038/s41598-017-12262-1" @default.
• W2756408218 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5605656" @default.
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