Matches in SemOpenAlex for { <https://semopenalex.org/work/W3035305202> ?p ?o ?g. }
- W3035305202 abstract "Abstract The autonomic nervous system (ANS) regulates tissue homeostasis and remodelling through antagonistic effects of noradrenergic sympathetic and cholinergic parasympathetic signalling. Despite numerous reports on the induction of sympathetic neurons from human pluripotent stem cells (hPSCs), no induction methods have effectively derived cholinergic parasympathetic neurons from hPSCs. Considering the antagonistic effects of noradrenergic and cholinergic inputs on target organs, both sympathetic and parasympathetic neurons are expected to be induced. This study aimed to develop a stepwise chemical induction method to induce sympathetic-like and parasympathetic-like ANS neurons. Autonomic specification was achieved through restricting signals inducing sensory or enteric neurogenesis and activating bone morphogenetic protein (BMP) signals. Global mRNA expression analyses after stepwise induction, including single-cell RNA-seq analysis of induced neurons and functional assays revealed that each induced sympathetic-like or parasympathetic-like neuron acquired pharmacological and electrophysiological functional properties with distinct marker expression. Further, we identified selective induction methods using appropriate seeding cell densities and neurotrophic factor concentrations. Neurons were individually induced, facilitating the regulation of the beating rates of hiPSC-derived cardiomyocytes in an antagonistic manner. The induction methods yield specific neuron types, and their influence on various tissues can be studied by co-cultured assays." @default.
- W3035305202 created "2020-06-19" @default.
- W3035305202 creator A5012898958 @default.
- W3035305202 creator A5019488402 @default.
- W3035305202 creator A5033654965 @default.
- W3035305202 creator A5046087604 @default.
- W3035305202 creator A5057608651 @default.
- W3035305202 creator A5070657030 @default.
- W3035305202 date "2020-06-11" @default.
- W3035305202 modified "2023-10-06" @default.
- W3035305202 title "Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating" @default.
- W3035305202 cites W1956941772 @default.
- W3035305202 cites W1970431562 @default.
- W3035305202 cites W1974773959 @default.
- W3035305202 cites W1975997945 @default.
- W3035305202 cites W1983072558 @default.
- W3035305202 cites W1983318575 @default.
- W3035305202 cites W1984878005 @default.
- W3035305202 cites W1986969720 @default.
- W3035305202 cites W1995319214 @default.
- W3035305202 cites W2001902784 @default.
- W3035305202 cites W2011024729 @default.
- W3035305202 cites W2014279663 @default.
- W3035305202 cites W2029663217 @default.
- W3035305202 cites W2035534054 @default.
- W3035305202 cites W2036564583 @default.
- W3035305202 cites W2036786455 @default.
- W3035305202 cites W2038567162 @default.
- W3035305202 cites W2049959255 @default.
- W3035305202 cites W2061787305 @default.
- W3035305202 cites W2074861977 @default.
- W3035305202 cites W2089210135 @default.
- W3035305202 cites W2090729997 @default.
- W3035305202 cites W2092683047 @default.
- W3035305202 cites W2099540110 @default.
- W3035305202 cites W2112832209 @default.
- W3035305202 cites W2122069573 @default.
- W3035305202 cites W2123046057 @default.
- W3035305202 cites W2126707217 @default.
- W3035305202 cites W2146119530 @default.
- W3035305202 cites W2147774449 @default.
- W3035305202 cites W2148909614 @default.
- W3035305202 cites W2150295764 @default.
- W3035305202 cites W2161771297 @default.
- W3035305202 cites W2263502969 @default.
- W3035305202 cites W2264853099 @default.
- W3035305202 cites W2442024627 @default.
- W3035305202 cites W2470296318 @default.
- W3035305202 cites W2555862905 @default.
- W3035305202 cites W2726594986 @default.
- W3035305202 cites W2778022289 @default.
- W3035305202 cites W2886748056 @default.
- W3035305202 cites W2888274827 @default.
- W3035305202 cites W2902626511 @default.
- W3035305202 cites W2911963127 @default.
- W3035305202 cites W4250943273 @default.
- W3035305202 doi "https://doi.org/10.1038/s41598-020-66303-3" @default.
- W3035305202 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7289887" @default.
- W3035305202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32528170" @default.
- W3035305202 hasPublicationYear "2020" @default.
- W3035305202 type Work @default.
- W3035305202 sameAs 3035305202 @default.
- W3035305202 citedByCount "16" @default.
- W3035305202 countsByYear W30353052022020 @default.
- W3035305202 countsByYear W30353052022021 @default.
- W3035305202 countsByYear W30353052022022 @default.
- W3035305202 countsByYear W30353052022023 @default.
- W3035305202 crossrefType "journal-article" @default.
- W3035305202 hasAuthorship W3035305202A5012898958 @default.
- W3035305202 hasAuthorship W3035305202A5019488402 @default.
- W3035305202 hasAuthorship W3035305202A5033654965 @default.
- W3035305202 hasAuthorship W3035305202A5046087604 @default.
- W3035305202 hasAuthorship W3035305202A5057608651 @default.
- W3035305202 hasAuthorship W3035305202A5070657030 @default.
- W3035305202 hasBestOaLocation W30353052021 @default.
- W3035305202 hasConcept C104317684 @default.
- W3035305202 hasConcept C104966671 @default.
- W3035305202 hasConcept C107459253 @default.
- W3035305202 hasConcept C134018914 @default.
- W3035305202 hasConcept C145103041 @default.
- W3035305202 hasConcept C160539049 @default.
- W3035305202 hasConcept C169760540 @default.
- W3035305202 hasConcept C170493617 @default.
- W3035305202 hasConcept C185263204 @default.
- W3035305202 hasConcept C22885893 @default.
- W3035305202 hasConcept C2776533406 @default.
- W3035305202 hasConcept C2777372248 @default.
- W3035305202 hasConcept C2777953023 @default.
- W3035305202 hasConcept C2778794669 @default.
- W3035305202 hasConcept C4746552 @default.
- W3035305202 hasConcept C55493867 @default.
- W3035305202 hasConcept C84393581 @default.
- W3035305202 hasConcept C86803240 @default.
- W3035305202 hasConceptScore W3035305202C104317684 @default.
- W3035305202 hasConceptScore W3035305202C104966671 @default.
- W3035305202 hasConceptScore W3035305202C107459253 @default.
- W3035305202 hasConceptScore W3035305202C134018914 @default.
- W3035305202 hasConceptScore W3035305202C145103041 @default.
- W3035305202 hasConceptScore W3035305202C160539049 @default.
- W3035305202 hasConceptScore W3035305202C169760540 @default.