Matches in SemOpenAlex for { <https://semopenalex.org/work/W105481595> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W105481595 endingPage "166" @default.
- W105481595 startingPage "155" @default.
- W105481595 abstract "This chapter describes the protocol for preparation of recombinant adenoviruses and infection of target cells to express transiently G-protein-coupled receptors or other proteins of interest. Adenoviruses are nonenveloped viruses containing a linear double-stranded DNA genome. Their life cycle does not normally involve integration into the host genome, rather they replicate as episomal elements in the nucleus of the host cell and consequently there is no risk of insertional mutagenesis. The wild-type adenovirus genome is approx 35 kb, of which up to 30 kb can be replaced by foreign DNA. Adenoviral vectors are very efficient at transducing the gene of interest in target cells in vitro and in vivo and can be produced at high titers (>10(11)/mL). The viral infection has a number of useful features: (1) the efficiency of gene transduction is very high (up to 100% in sensitive cells). (2) The infection is easy and does not alter physically the cell membrane for gene transduction. (3) It is possible to infect cells that are resistant to transfection with plasmids (including nondividing cells)." @default.
- W105481595 created "2016-06-24" @default.
- W105481595 creator A5044214963 @default.
- W105481595 creator A5055286766 @default.
- W105481595 date "2004-02-09" @default.
- W105481595 modified "2023-09-23" @default.
- W105481595 title "Viral Infection Protocols" @default.
- W105481595 cites W1616824889 @default.
- W105481595 cites W2035600956 @default.
- W105481595 cites W2044296969 @default.
- W105481595 cites W2070420177 @default.
- W105481595 cites W2132417094 @default.
- W105481595 cites W2084017165 @default.
- W105481595 cites W2133916339 @default.
- W105481595 doi "https://doi.org/10.1385/1-59259-754-8:155" @default.
- W105481595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15250491" @default.
- W105481595 hasPublicationYear "2004" @default.
- W105481595 type Work @default.
- W105481595 sameAs 105481595 @default.
- W105481595 citedByCount "2" @default.
- W105481595 crossrefType "book-chapter" @default.
- W105481595 hasAuthorship W105481595A5044214963 @default.
- W105481595 hasAuthorship W105481595A5055286766 @default.
- W105481595 hasConcept C104317684 @default.
- W105481595 hasConcept C107635520 @default.
- W105481595 hasConcept C141231307 @default.
- W105481595 hasConcept C145085861 @default.
- W105481595 hasConcept C15152581 @default.
- W105481595 hasConcept C153911025 @default.
- W105481595 hasConcept C159047783 @default.
- W105481595 hasConcept C22744801 @default.
- W105481595 hasConcept C2522874641 @default.
- W105481595 hasConcept C2776954459 @default.
- W105481595 hasConcept C40767141 @default.
- W105481595 hasConcept C54009773 @default.
- W105481595 hasConcept C54355233 @default.
- W105481595 hasConcept C552990157 @default.
- W105481595 hasConcept C55493867 @default.
- W105481595 hasConcept C86803240 @default.
- W105481595 hasConcept C95444343 @default.
- W105481595 hasConceptScore W105481595C104317684 @default.
- W105481595 hasConceptScore W105481595C107635520 @default.
- W105481595 hasConceptScore W105481595C141231307 @default.
- W105481595 hasConceptScore W105481595C145085861 @default.
- W105481595 hasConceptScore W105481595C15152581 @default.
- W105481595 hasConceptScore W105481595C153911025 @default.
- W105481595 hasConceptScore W105481595C159047783 @default.
- W105481595 hasConceptScore W105481595C22744801 @default.
- W105481595 hasConceptScore W105481595C2522874641 @default.
- W105481595 hasConceptScore W105481595C2776954459 @default.
- W105481595 hasConceptScore W105481595C40767141 @default.
- W105481595 hasConceptScore W105481595C54009773 @default.
- W105481595 hasConceptScore W105481595C54355233 @default.
- W105481595 hasConceptScore W105481595C552990157 @default.
- W105481595 hasConceptScore W105481595C55493867 @default.
- W105481595 hasConceptScore W105481595C86803240 @default.
- W105481595 hasConceptScore W105481595C95444343 @default.
- W105481595 hasLocation W1054815951 @default.
- W105481595 hasLocation W1054815952 @default.
- W105481595 hasOpenAccess W105481595 @default.
- W105481595 hasPrimaryLocation W1054815951 @default.
- W105481595 hasRelatedWork W1628389778 @default.
- W105481595 hasRelatedWork W1786775465 @default.
- W105481595 hasRelatedWork W1970317561 @default.
- W105481595 hasRelatedWork W2003306608 @default.
- W105481595 hasRelatedWork W2015111081 @default.
- W105481595 hasRelatedWork W2044629552 @default.
- W105481595 hasRelatedWork W2062622002 @default.
- W105481595 hasRelatedWork W2069068769 @default.
- W105481595 hasRelatedWork W2072397337 @default.
- W105481595 hasRelatedWork W2075328891 @default.
- W105481595 hasRelatedWork W2075921029 @default.
- W105481595 hasRelatedWork W2084210336 @default.
- W105481595 hasRelatedWork W2088443205 @default.
- W105481595 hasRelatedWork W2088689494 @default.
- W105481595 hasRelatedWork W2121944814 @default.
- W105481595 hasRelatedWork W2133936082 @default.
- W105481595 hasRelatedWork W2148511634 @default.
- W105481595 hasRelatedWork W2151856742 @default.
- W105481595 hasRelatedWork W2328238314 @default.
- W105481595 hasRelatedWork W2343278400 @default.
- W105481595 isParatext "false" @default.
- W105481595 isRetracted "false" @default.
- W105481595 magId "105481595" @default.
- W105481595 workType "book-chapter" @default.