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- W1553976218 abstract "A method is described for the determination of steroid profiles from urine by means of gas chromatography using high-efficiency glass capillary columns. The accuracy and reproducibility of essential steps in the sample preparation (extraction of steroids and steroid conjugates by means of XAD-2, enzymatic hydrolysis with Helix pomatia juice, solvolysis in acidified ethyl acetate and alkali wash) are established using different endogenously labelled urine samples, obtained from normal subjects to whom labelled steroids had been administered. Preliminary results are given on the reproducibility of the derivatization procedure (formation of methoxime-trimethylsilyl (MO-TMS) ethers), the gas chromatographic analysis and the whole method. Two procedures for the purification of MO-TMS steroid derivatives are compared. Application of the method to urine samples of patients with various endocrine disorders is included." @default.
- W1553976218 created "2016-06-24" @default.
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- W1553976218 date "1978-11-01" @default.
- W1553976218 modified "2023-10-16" @default.
- W1553976218 title "Quantitative analysis of steroid profiles from urine by capillary gas chromatography" @default.
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- W1553976218 doi "https://doi.org/10.1016/s0378-4347(00)81196-2" @default.
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