FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1593924964> ?p ?o ?g. }

• W1593924964 endingPage "11799" @default.
• W1593924964 startingPage "11794" @default.
• W1593924964 abstract "A panel of anti-thyroid hormone receptor (TR) antisera were generated to allow direct assay of the concentrations of the alpha 1 and beta 1 receptor isoforms in nuclear extracts from adult rat liver, kidney, brain and heart, and fetal brain. An antiserum, immunoglobulin G (IgG)-beta 1, raised against amino acid sequence 62-92 of the rat TR-beta 1 specifically precipitated only TR-beta 1 in vitro translation products. A second antiserum, IgG-alpha 1/beta, generated against a sequence that is identical in the ligand binding region of rat TR-alpha 1 and TR-beta isoforms immunoprecipitated both TR-alpha 1 and -beta 1 translation products. These IgG preparations were used to specifically immunoprecipitate thyroid hormone receptor binding activity from nuclear extracts. IgG-beta 1 cleared almost 80%, and the IgG-alpha 1/beta immunoprecipitated nearly all binding from hepatic nuclear extracts. This distribution of TR protein, 80% beta 1 and 20% alpha 1, is the same as previously reported for their respective mRNAs in liver. In heart, kidney, and brain IgG-beta 1 cleared 45, 43, and 28% of total binding, respectively, and IgG-alpha 1/beta cleared all T3 binding activity from these tissues. In agreement with an earlier study, marked variations in specific protein/mRNA ratios were noted among these tissues. Consistent with our earlier report of the presence of only very low levels of TR-beta 1 mRNA in fetal brain, IgG-beta 1 cleared just 5% of binding in this tissue. Studies using an antiserum (IgG-ch) generated against homologous segments of the hinge region in both TR-alpha 1 and -beta 1 yielded results which contrasted sharply with those of IgG-alpha 1/beta. Whereas IgG-ch could also immunoprecipitate virtually all binding from hepatic extracts it cleared only 40-50% of binding from the other tissues, including fetal brain in which TR-alpha 1 accounts for greater than 90% of binding protein. The data suggest the presence of posttranslational modification of the TR-alpha 1 protein in the hinge region, consistent with the presence in this segment of potential phosphorylation sites." @default.
• W1593924964 created "2016-06-24" @default.
• W1593924964 creator A5039311948 @default.
• W1593924964 creator A5059616408 @default.
• W1593924964 creator A5068059911 @default.
• W1593924964 creator A5081208916 @default.
• W1593924964 date "1992-06-01" @default.
• W1593924964 modified "2023-10-05" @default.
• W1593924964 title "Quantitation of rat tissue thyroid hormone binding receptor isoforms by immunoprecipitation of nuclear triiodothyronine binding capacity." @default.
• W1593924964 cites W1547202268 @default.
• W1593924964 cites W1565210265 @default.
• W1593924964 cites W1565592850 @default.
• W1593924964 cites W1568323065 @default.
• W1593924964 cites W1569915920 @default.
• W1593924964 cites W1596570248 @default.
• W1593924964 cites W1695605475 @default.
• W1593924964 cites W1970895519 @default.
• W1593924964 cites W1974639601 @default.
• W1593924964 cites W1983450433 @default.
• W1593924964 cites W1990675136 @default.
• W1593924964 cites W2015852574 @default.
• W1593924964 cites W2020984531 @default.
• W1593924964 cites W2024308660 @default.
• W1593924964 cites W2028549678 @default.
• W1593924964 cites W2058232931 @default.
• W1593924964 cites W2058832065 @default.
• W1593924964 cites W2077580619 @default.
• W1593924964 cites W2080046379 @default.
• W1593924964 cites W2081585400 @default.
• W1593924964 cites W2090024435 @default.
• W1593924964 cites W2171315857 @default.
• W1593924964 cites W4246404158 @default.
• W1593924964 cites W979725628 @default.
• W1593924964 doi "https://doi.org/10.1016/s0021-9258(19)49768-8" @default.
• W1593924964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1601852" @default.
• W1593924964 hasPublicationYear "1992" @default.
• W1593924964 type Work @default.
• W1593924964 sameAs 1593924964 @default.
• W1593924964 citedByCount "156" @default.
• W1593924964 countsByYear W15939249642012 @default.
• W1593924964 countsByYear W15939249642013 @default.
• W1593924964 countsByYear W15939249642014 @default.
• W1593924964 countsByYear W15939249642015 @default.
• W1593924964 countsByYear W15939249642016 @default.
• W1593924964 countsByYear W15939249642017 @default.
• W1593924964 countsByYear W15939249642018 @default.
• W1593924964 countsByYear W15939249642019 @default.
• W1593924964 countsByYear W15939249642021 @default.
• W1593924964 countsByYear W15939249642022 @default.
• W1593924964 countsByYear W15939249642023 @default.
• W1593924964 crossrefType "journal-article" @default.
• W1593924964 hasAuthorship W1593924964A5039311948 @default.
• W1593924964 hasAuthorship W1593924964A5059616408 @default.
• W1593924964 hasAuthorship W1593924964A5068059911 @default.
• W1593924964 hasAuthorship W1593924964A5081208916 @default.
• W1593924964 hasBestOaLocation W15939249641 @default.
• W1593924964 hasConcept C104317684 @default.
• W1593924964 hasConcept C109115496 @default.
• W1593924964 hasConcept C121608353 @default.
• W1593924964 hasConcept C126322002 @default.
• W1593924964 hasConcept C134018914 @default.
• W1593924964 hasConcept C149574134 @default.
• W1593924964 hasConcept C160860887 @default.
• W1593924964 hasConcept C170493617 @default.
• W1593924964 hasConcept C185592680 @default.
• W1593924964 hasConcept C23589133 @default.
• W1593924964 hasConcept C2778288131 @default.
• W1593924964 hasConcept C526584372 @default.
• W1593924964 hasConcept C530470458 @default.
• W1593924964 hasConcept C53345823 @default.
• W1593924964 hasConcept C55493867 @default.
• W1593924964 hasConcept C63932345 @default.
• W1593924964 hasConcept C71315377 @default.
• W1593924964 hasConcept C71829478 @default.
• W1593924964 hasConcept C71924100 @default.
• W1593924964 hasConcept C86339819 @default.
• W1593924964 hasConcept C86803240 @default.
• W1593924964 hasConceptScore W1593924964C104317684 @default.
• W1593924964 hasConceptScore W1593924964C109115496 @default.
• W1593924964 hasConceptScore W1593924964C121608353 @default.
• W1593924964 hasConceptScore W1593924964C126322002 @default.
• W1593924964 hasConceptScore W1593924964C134018914 @default.
• W1593924964 hasConceptScore W1593924964C149574134 @default.
• W1593924964 hasConceptScore W1593924964C160860887 @default.
• W1593924964 hasConceptScore W1593924964C170493617 @default.
• W1593924964 hasConceptScore W1593924964C185592680 @default.
• W1593924964 hasConceptScore W1593924964C23589133 @default.
• W1593924964 hasConceptScore W1593924964C2778288131 @default.
• W1593924964 hasConceptScore W1593924964C526584372 @default.
• W1593924964 hasConceptScore W1593924964C530470458 @default.
• W1593924964 hasConceptScore W1593924964C53345823 @default.
• W1593924964 hasConceptScore W1593924964C55493867 @default.
• W1593924964 hasConceptScore W1593924964C63932345 @default.
• W1593924964 hasConceptScore W1593924964C71315377 @default.
• W1593924964 hasConceptScore W1593924964C71829478 @default.
• W1593924964 hasConceptScore W1593924964C71924100 @default.
• W1593924964 hasConceptScore W1593924964C86339819 @default.
• W1593924964 hasConceptScore W1593924964C86803240 @default.

• next