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• W160494865 abstract "A minor, more electronegatively charged, sub-fraction (LDL-) was isolated from plasma LDL by ion exchange HPLC and characterized as a mildly oxidized LDL. In samples obtained from normal donors HPLC analysis of OPA derivatized amino-acids, reverse phase HPLC of phospho-lipids and GC analysis of oxysterols was performed in both normally charged LDL (nLDL) and LDL-. In LDL- a significant decrease of aspartic acid, histidine, threonine, isoleucine, leucine and lysine was found, whereas serine, glycine and alanine increased. Among phospholipid classes there was a decrease in phosphatidylcholine and an increase in lysophosphatidylcholine in LDL- as compared to nLDL; also the percentage content of phosphoinositol, phosphatidtlserine and phosphatidylhetanolamine was increased in LDL-. Furthermore, a specific increase in cholestan-3,5-diene-7-one and 7-β-hydroxycholesterol, along with an increase in total oxysterols were found in LDL-. Some of the observed characteristics (reduction of histidine and lysine residues, increase of lyso-phosphatidylcholine and presence of oxysterols) have been previously reported in LDL oxidized by metal ions or by endothelial cells cultures. These data support the hypothesis that the LDL- subtraction may originate from native LDL, through a mild oxidative process occurring “in vivo”." @default.
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• W160494865 date "1993-01-01" @default.
• W160494865 modified "2023-09-27" @default.
• W160494865 title "Oxidative Damage to Circulating LDL" @default.
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• W160494865 doi "https://doi.org/10.1007/978-94-011-1703-6_25" @default.
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