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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968666783> ?p ?o ?g. }

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• W1968666783 abstract "A pyrene-labeled Zn2+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amounts as low as 10 ng and short DNA strands (∼300 b.p.) are detectable. Gel extraction as a standard technique in molecular biology was successfully performed after staining with Zn2+-cyclen-pyrene. Cytotoxicity tests on HeLa and V-79 cells reveal that the zinc-cyclen pyrene probe is significant less toxic compared to ethidium bromide." @default.
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• W1968666783 date "2010-10-06" @default.
• W1968666783 modified "2023-10-17" @default.
• W1968666783 title "DNA Staining in Agarose Gels with ZN²⁺-Cyclen-Pyrene" @default.
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• W1968666783 doi "https://doi.org/10.1080/15257770.2010.515282" @default.
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