FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968873371> ?p ?o ?g. }

• W1968873371 endingPage "329" @default.
• W1968873371 startingPage "321" @default.
• W1968873371 abstract "1 The human adenocarcinoma cell line, HT-29, has been stably transfected with the cDNA sequence for the rat neuropeptide Y (NPY) Y1 receptor, and three Y1 clones (Y1-4, Y1-7 and Y1-16) have been isolated which express high levels of specific [125I]-PYY binding. We have studied the functional responses or lack of responses to peptide YY (PYY) and its analogues in the three transfected clones and HT-29 wild type (wt) cells. 2 Vasoactive intestinal polypeptide (VIP) produced long-lasting increases in short-circuit current (SCC) in both HT-29 wt cells and the Y1 clones. VIP EC50 values were 8.4–11.7 nM in all four cases. The elevation in SCC after a maximal concentration of VIP (30 nM) was significantly greater in Y1-7 cells than in either HT-29 wt epithelia or the other Y1 cell lines. 3 PYY (100 nM) and human pancreatic polypeptide (hPP; 1 μm) were ineffective in HT-29 wt cells under either basal or stimulated conditions. In contrast, basolateral additions of PYY reduced both basal and VIP-stimulated SCC in all three Y1 clones. After VIP, the PYY EC50 values (in nM) were 18.6 in Y1-4, 8.0 in Y1-7 and 52.5 in Y1-16. hPP (1 μm) produced only small and transient responses in each transfected cell type. 4 The Y1 receptor agonist, [Leu31, Pro34] NPY (1 μm) was also effective in the three Y1 cell lines. In the Y1-7 clone the EC50 value for the effect of this peptide was 149 nM, 18.6 fold less potent than PYY. 5 PYY and the Y1-selective non-peptide antagonist, BIBP 3226 displaced [125I]-PYY binding from Y1-7 cell membranes with Ki values of 2.0 and 3.1 nM respectively. In the Y1-7 clone, BIBP 3226 fully inhibited the reductions in VIP-stimulated SCC induced by 30 nM PYY, with an IC50 of 27.2 nM and 30 nM BIBP 3226 caused a parallel rightward shift on the PYY concentration-response curve, with an approximate pKB of 8.0. 6 HT-29 clones stably expressing the Y1 receptor therefore show responses to PYY and its analogues that are characteristic of that subtype, and the Y1-7 clone in particular will be useful in the assessment of novel Y1-specific drugs. This approach will also allow the functional study of NPY Y1 receptors with selected mutations." @default.
• W1968873371 created "2016-06-24" @default.
• W1968873371 creator A5009862868 @default.
• W1968873371 creator A5032585100 @default.
• W1968873371 date "1996-09-01" @default.
• W1968873371 modified "2023-09-27" @default.
• W1968873371 title "The functional investigation of a human adenocarcinoma cell line, stably transfected with the neuropeptide Y Y1 receptor" @default.
• W1968873371 cites W1502386136 @default.
• W1968873371 cites W1530935792 @default.
• W1968873371 cites W1538620682 @default.
• W1968873371 cites W1544954412 @default.
• W1968873371 cites W1763239887 @default.
• W1968873371 cites W1814803903 @default.
• W1968873371 cites W1846247956 @default.
• W1968873371 cites W1907886138 @default.
• W1968873371 cites W1963816294 @default.
• W1968873371 cites W1970172671 @default.
• W1968873371 cites W1970249811 @default.
• W1968873371 cites W1970814454 @default.
• W1968873371 cites W1973972465 @default.
• W1968873371 cites W1975204220 @default.
• W1968873371 cites W1988628530 @default.
• W1968873371 cites W1989463917 @default.
• W1968873371 cites W1990601982 @default.
• W1968873371 cites W1994488695 @default.
• W1968873371 cites W2005187945 @default.
• W1968873371 cites W2024405630 @default.
• W1968873371 cites W2030598793 @default.
• W1968873371 cites W2034995830 @default.
• W1968873371 cites W2039866097 @default.
• W1968873371 cites W2043060620 @default.
• W1968873371 cites W2044583182 @default.
• W1968873371 cites W2058536739 @default.
• W1968873371 cites W2062060671 @default.
• W1968873371 cites W2070569836 @default.
• W1968873371 cites W2073503969 @default.
• W1968873371 cites W2087381943 @default.
• W1968873371 cites W2092743131 @default.
• W1968873371 cites W2108896220 @default.
• W1968873371 cites W2118678170 @default.
• W1968873371 cites W2128635872 @default.
• W1968873371 cites W2133354537 @default.
• W1968873371 cites W2144023847 @default.
• W1968873371 cites W2163143518 @default.
• W1968873371 cites W2287018070 @default.
• W1968873371 cites W2318018840 @default.
• W1968873371 cites W4248303125 @default.
• W1968873371 cites W4293247451 @default.
• W1968873371 doi "https://doi.org/10.1111/j.1476-5381.1996.tb15989.x" @default.
• W1968873371 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1915860" @default.
• W1968873371 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8886416" @default.
• W1968873371 hasPublicationYear "1996" @default.
• W1968873371 type Work @default.
• W1968873371 sameAs 1968873371 @default.
• W1968873371 citedByCount "6" @default.
• W1968873371 crossrefType "journal-article" @default.
• W1968873371 hasAuthorship W1968873371A5009862868 @default.
• W1968873371 hasAuthorship W1968873371A5032585100 @default.
• W1968873371 hasBestOaLocation W19688733711 @default.
• W1968873371 hasConcept C118303440 @default.
• W1968873371 hasConcept C126322002 @default.
• W1968873371 hasConcept C134018914 @default.
• W1968873371 hasConcept C153911025 @default.
• W1968873371 hasConcept C170493617 @default.
• W1968873371 hasConcept C185592680 @default.
• W1968873371 hasConcept C204232928 @default.
• W1968873371 hasConcept C2777858994 @default.
• W1968873371 hasConcept C2778108375 @default.
• W1968873371 hasConcept C2778563252 @default.
• W1968873371 hasConcept C2778938600 @default.
• W1968873371 hasConcept C2780796126 @default.
• W1968873371 hasConcept C54009773 @default.
• W1968873371 hasConcept C54355233 @default.
• W1968873371 hasConcept C552990157 @default.
• W1968873371 hasConcept C55493867 @default.
• W1968873371 hasConcept C71315377 @default.
• W1968873371 hasConcept C71924100 @default.
• W1968873371 hasConcept C81089528 @default.
• W1968873371 hasConcept C81885089 @default.
• W1968873371 hasConcept C8330315 @default.
• W1968873371 hasConcept C86803240 @default.
• W1968873371 hasConcept C93984277 @default.
• W1968873371 hasConceptScore W1968873371C118303440 @default.
• W1968873371 hasConceptScore W1968873371C126322002 @default.
• W1968873371 hasConceptScore W1968873371C134018914 @default.
• W1968873371 hasConceptScore W1968873371C153911025 @default.
• W1968873371 hasConceptScore W1968873371C170493617 @default.
• W1968873371 hasConceptScore W1968873371C185592680 @default.
• W1968873371 hasConceptScore W1968873371C204232928 @default.
• W1968873371 hasConceptScore W1968873371C2777858994 @default.
• W1968873371 hasConceptScore W1968873371C2778108375 @default.
• W1968873371 hasConceptScore W1968873371C2778563252 @default.
• W1968873371 hasConceptScore W1968873371C2778938600 @default.
• W1968873371 hasConceptScore W1968873371C2780796126 @default.
• W1968873371 hasConceptScore W1968873371C54009773 @default.
• W1968873371 hasConceptScore W1968873371C54355233 @default.
• W1968873371 hasConceptScore W1968873371C552990157 @default.
• W1968873371 hasConceptScore W1968873371C55493867 @default.

• next