Matches in SemOpenAlex for { <https://semopenalex.org/work/W1971722997> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W1971722997 endingPage "1716" @default.
- W1971722997 startingPage "1707" @default.
- W1971722997 abstract "Four neurotrophic factors, important for survival and function of neurons, bind a common receptor, the 75-kDa neurotrophin receptor (NTR). An O-glycosylated peptide connects the ligand-binding domain of NTR to its transmembrane helix. This peptide, the transmembrane helix, and intracellular sequences are highly conserved in vertebrate evolution. To investigate the structure and function of O-glycosylation on NTR, we produced the extracellular domains by expression in mammalian cells. Addition during biosynthesis of O-linked glycans was evaluated, and structures were characterized by lectin blotting and glycosidase digestion. Effects of disialylation, deglycosylation, and lectin attachment on the equilibrium binding constant were measured. Addition of O-linked glycans during biosynthesis was found to have a large effect on NTR structure assessed by mobility in polyacrylamide gels. NTR O-linked glycans synthesized by cultured cells had the structure (NeuNAc)(1-2-) Gal beta 1-3GalNAc. Modification of the O-linked oligosaccharide produced small, possibly significant effects on the binding constant of NTR for nerve growth factor. The results are discussed in reference to a potential role for the stalk region in ligand binding and signaling." @default.
- W1971722997 created "2016-06-24" @default.
- W1971722997 creator A5042612584 @default.
- W1971722997 creator A5053276616 @default.
- W1971722997 creator A5068298685 @default.
- W1971722997 creator A5080479221 @default.
- W1971722997 date "2002-11-23" @default.
- W1971722997 modified "2023-10-13" @default.
- W1971722997 title "O-Linked Oligosaccharide on the 75-kDa Neurotrophin Receptor" @default.
- W1971722997 doi "https://doi.org/10.1046/j.1471-4159.1996.66041707.x" @default.
- W1971722997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8627329" @default.
- W1971722997 hasPublicationYear "2002" @default.
- W1971722997 type Work @default.
- W1971722997 sameAs 1971722997 @default.
- W1971722997 citedByCount "14" @default.
- W1971722997 countsByYear W19717229972021 @default.
- W1971722997 countsByYear W19717229972022 @default.
- W1971722997 countsByYear W19717229972023 @default.
- W1971722997 crossrefType "journal-article" @default.
- W1971722997 hasAuthorship W1971722997A5042612584 @default.
- W1971722997 hasAuthorship W1971722997A5053276616 @default.
- W1971722997 hasAuthorship W1971722997A5068298685 @default.
- W1971722997 hasAuthorship W1971722997A5080479221 @default.
- W1971722997 hasConcept C108625454 @default.
- W1971722997 hasConcept C116569031 @default.
- W1971722997 hasConcept C118892022 @default.
- W1971722997 hasConcept C170493617 @default.
- W1971722997 hasConcept C185592680 @default.
- W1971722997 hasConcept C206212055 @default.
- W1971722997 hasConcept C24530287 @default.
- W1971722997 hasConcept C2777313579 @default.
- W1971722997 hasConcept C2777726330 @default.
- W1971722997 hasConcept C2780758401 @default.
- W1971722997 hasConcept C28406088 @default.
- W1971722997 hasConcept C30324644 @default.
- W1971722997 hasConcept C55493867 @default.
- W1971722997 hasConcept C86803240 @default.
- W1971722997 hasConcept C92020748 @default.
- W1971722997 hasConcept C95444343 @default.
- W1971722997 hasConceptScore W1971722997C108625454 @default.
- W1971722997 hasConceptScore W1971722997C116569031 @default.
- W1971722997 hasConceptScore W1971722997C118892022 @default.
- W1971722997 hasConceptScore W1971722997C170493617 @default.
- W1971722997 hasConceptScore W1971722997C185592680 @default.
- W1971722997 hasConceptScore W1971722997C206212055 @default.
- W1971722997 hasConceptScore W1971722997C24530287 @default.
- W1971722997 hasConceptScore W1971722997C2777313579 @default.
- W1971722997 hasConceptScore W1971722997C2777726330 @default.
- W1971722997 hasConceptScore W1971722997C2780758401 @default.
- W1971722997 hasConceptScore W1971722997C28406088 @default.
- W1971722997 hasConceptScore W1971722997C30324644 @default.
- W1971722997 hasConceptScore W1971722997C55493867 @default.
- W1971722997 hasConceptScore W1971722997C86803240 @default.
- W1971722997 hasConceptScore W1971722997C92020748 @default.
- W1971722997 hasConceptScore W1971722997C95444343 @default.
- W1971722997 hasIssue "4" @default.
- W1971722997 hasLocation W19717229971 @default.
- W1971722997 hasLocation W19717229972 @default.
- W1971722997 hasOpenAccess W1971722997 @default.
- W1971722997 hasPrimaryLocation W19717229971 @default.
- W1971722997 hasRelatedWork W1176648687 @default.
- W1971722997 hasRelatedWork W1531549338 @default.
- W1971722997 hasRelatedWork W1555877029 @default.
- W1971722997 hasRelatedWork W1975019028 @default.
- W1971722997 hasRelatedWork W2064742682 @default.
- W1971722997 hasRelatedWork W2089644524 @default.
- W1971722997 hasRelatedWork W2247651258 @default.
- W1971722997 hasRelatedWork W2417600213 @default.
- W1971722997 hasRelatedWork W3100688369 @default.
- W1971722997 hasRelatedWork W3174898703 @default.
- W1971722997 hasVolume "66" @default.
- W1971722997 isParatext "false" @default.
- W1971722997 isRetracted "false" @default.
- W1971722997 magId "1971722997" @default.
- W1971722997 workType "article" @default.