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• W1974538526 abstract "The retention mechanism of guanidino compounds on a porous graphitic carbon seemed to be mainly hydrophobic interaction, according to the retention factors in buffer solutions and the results of an analysis by computational chemical calculation using molecular mechanics (MM2). The baseline separation of ten guanidino compounds was achieved by the addition of a hydrophobic counterion. The retention mechanism may be dynamic ion-exchange. The stable system was applied to the analysis of guanidino compounds in serum from nephritic patients. The effluent was monitored by a post-column labeling detection method using ninhydrin. The detection limit of guanidino compounds was a few picomoles; however, that of creatinine was one hundredth of those of the other compounds. The reproducibilities of the peak height and area of the ten guanidino compounds using gradient elution were quite high, and the standard deviations were within a few percent (n=5), except for creatinine. The recovery of the compounds from serum was more than 90% (n=5). The reproducibility of retention times was within 1% (n=5)." @default.
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• W1974538526 date "1998-04-01" @default.
• W1974538526 modified "2023-10-14" @default.
• W1974538526 title "Liquid chromatography of guanidino compounds using a porous graphite carbon column and application to their analysis in serum" @default.
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• W1974538526 doi "https://doi.org/10.1016/s0378-4347(97)00576-8" @default.
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