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- W1979363512 abstract "The P/sup 32/-decay inactivation technique was used to study the wild- type sex factor of E. coli K12, F/sub 1/, and a variant sex factor, F/sub 2/. Both are sensitive to decay of incorporated P/sup 32/. Labeled sex factors transferred into unlabeled recipient cells were shown to lose function on storage; inactivation kinetics were described by concave exponential curves, indicating the transfer of only one sex factor particle to any one recipient. Mitomycin C was employed as a specific inhibitor of net DNA synthesis. A concentration of the antibiotic having no effect on net RNA or protein synthesis was found to inhibit incorporation of W/sub 2/ into F/sub 1/ particles and nuclei of F/sub 1/ donor cells 84 and 69%, respectively, as determined by relative rates of P/sup 32/-decay inactivation of the genetic elements labeled in the presence and absence of mitomycin. An inhibition of incorporation of P/sup 32/ into F/sub 2/ and nuclei of donor cells of 88 and 64%, respectively, was observed. DNA is thus an essential constituent of the sex factors; RNA, in amounts detectable by the methods employed, is not. A comparison of the relative rates of P/sup 32/- decay inactivation of F/sub 1/, F/sub 2/,more » and bacteriophages T1 and T4 indicated that F/sub 1/ comprises between 8.5 x 10/sup 4/ and 2.5 x 10/sup 5/ P atoms while F/sub 2/ comprises between 1.9 x 10/sup 5/ and 5.4 x 10/sup 5/, upper limits being the more probable. This difference in size between the two sex factors was also demonstrated by the respective times required for transfer to recipients. The rate of sex factor transfer was estimated to be between 8.7 x 10/sup 4/ and 1.9 x 10/sup 5/ P atoms/min. This indicates that transfer is not initiated until more than six min after donor and recipient cells are mixed, although contacts between parent cells are considered to be formed as soon as mixing occurs. (BBB)« less" @default.
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- W1979363512 date "1963-06-01" @default.
- W1979363512 modified "2023-09-26" @default.
- W1979363512 title "Studies on the chemical nature and size of sex factors of Escherichia coli K12" @default.
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- W1979363512 doi "https://doi.org/10.1016/s0022-2836(63)80060-1" @default.
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