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- W1983248229 endingPage "e1002197" @default.
- W1983248229 startingPage "e1002197" @default.
- W1983248229 abstract "Apicomplexans are obligate intracellular parasites that invade host cells by an active process leading to the formation of a non-fusogenic parasitophorous vacuole (PV) where the parasite replicates within the host cell. The rhomboid family of proteases cleaves substrates within their transmembrane domains and has been implicated in the invasion process. Although its exact function is unknown, Plasmodium ROM1 is hypothesized to play a role during invasion based on its microneme localization and its ability to cleave essential invasion adhesins. Using the rodent malaria model, Plasmodium yoelii, we carried out detailed quantitative analysis of pyrom1 deficient parasites during the Plasmodium lifecycle. Pyrom1(-) parasites are attenuated during erythrocytic and hepatic stages but progress normally through the mosquito vector with normal counts of oocyst and salivary gland sporozoites. Pyrom1 steady state mRNA levels are upregulated 20-fold in salivary gland sporozoites compared to blood stages. We show that pyrom1(-) sporozoites are capable of gliding motility and traversing host cells normally. Wildtype and pyrom1(-) sporozoites do not differ in the rate of entry into Hepa1–6 hepatocytes. Within the first twelve hours of hepatic development, however, only 50% pyrom1(-) parasites have developed into exoerythrocytic forms. Immunofluorescence microscopy using the PVM marker UIS4 and transmission electron microscopy reveal that the PV of a significant fraction of pyrom1(-) parasites are morphologically aberrant shortly after invasion. We propose a novel function for PyROM1 as a protease that promotes proper PV modification to allow parasite development and replication in a suitable environment within the mammalian host." @default.
- W1983248229 created "2016-06-24" @default.
- W1983248229 creator A5012073462 @default.
- W1983248229 creator A5042341113 @default.
- W1983248229 creator A5052119516 @default.
- W1983248229 creator A5072344550 @default.
- W1983248229 date "2011-09-01" @default.
- W1983248229 modified "2023-10-16" @default.
- W1983248229 title "Plasmodium Protease ROM1 Is Important for Proper Formation of the Parasitophorous Vacuole" @default.
- W1983248229 cites W1522914336 @default.
- W1983248229 cites W1580280659 @default.
- W1983248229 cites W1603230814 @default.
- W1983248229 cites W1905994259 @default.
- W1983248229 cites W1963847487 @default.
- W1983248229 cites W1965111762 @default.
- W1983248229 cites W1965804686 @default.
- W1983248229 cites W1966867282 @default.
- W1983248229 cites W1971737237 @default.
- W1983248229 cites W1973529656 @default.
- W1983248229 cites W1987415337 @default.
- W1983248229 cites W1990402424 @default.
- W1983248229 cites W1995467090 @default.
- W1983248229 cites W1996143581 @default.
- W1983248229 cites W2005123510 @default.
- W1983248229 cites W2006171752 @default.
- W1983248229 cites W2015186773 @default.
- W1983248229 cites W2019462270 @default.
- W1983248229 cites W2021528981 @default.
- W1983248229 cites W2022114463 @default.
- W1983248229 cites W2029131750 @default.
- W1983248229 cites W2031521449 @default.
- W1983248229 cites W2033155615 @default.
- W1983248229 cites W2038121339 @default.
- W1983248229 cites W2039108744 @default.
- W1983248229 cites W2039481952 @default.
- W1983248229 cites W2043052053 @default.
- W1983248229 cites W2046391188 @default.
- W1983248229 cites W2047308369 @default.
- W1983248229 cites W2048171163 @default.
- W1983248229 cites W2051719089 @default.
- W1983248229 cites W2052272127 @default.
- W1983248229 cites W2054189292 @default.
- W1983248229 cites W2054785775 @default.
- W1983248229 cites W2057457553 @default.
- W1983248229 cites W2059696825 @default.
- W1983248229 cites W2060564531 @default.
- W1983248229 cites W2064005572 @default.
- W1983248229 cites W2064769066 @default.
- W1983248229 cites W2064864983 @default.
- W1983248229 cites W2065534196 @default.
- W1983248229 cites W2066838934 @default.
- W1983248229 cites W2067517994 @default.
- W1983248229 cites W2069498930 @default.
- W1983248229 cites W2071285311 @default.
- W1983248229 cites W2071979597 @default.
- W1983248229 cites W2071990248 @default.
- W1983248229 cites W2077244062 @default.
- W1983248229 cites W2085705809 @default.
- W1983248229 cites W2087585115 @default.
- W1983248229 cites W2088064497 @default.
- W1983248229 cites W2090169676 @default.
- W1983248229 cites W2093085760 @default.
- W1983248229 cites W2093867776 @default.
- W1983248229 cites W2094725247 @default.
- W1983248229 cites W2095182820 @default.
- W1983248229 cites W2097370940 @default.
- W1983248229 cites W2099528288 @default.
- W1983248229 cites W2099872182 @default.
- W1983248229 cites W2107277218 @default.
- W1983248229 cites W2110243445 @default.
- W1983248229 cites W2110649122 @default.
- W1983248229 cites W2111078556 @default.
- W1983248229 cites W2112047656 @default.
- W1983248229 cites W2113206880 @default.
- W1983248229 cites W2113424859 @default.
- W1983248229 cites W2116168912 @default.
- W1983248229 cites W2117691144 @default.
- W1983248229 cites W2119878454 @default.
- W1983248229 cites W2121413704 @default.
- W1983248229 cites W2131117238 @default.
- W1983248229 cites W2138623091 @default.
- W1983248229 cites W2142106325 @default.
- W1983248229 cites W2153823761 @default.
- W1983248229 cites W2155362327 @default.
- W1983248229 cites W2156352436 @default.
- W1983248229 cites W2158459142 @default.
- W1983248229 cites W2159579370 @default.
- W1983248229 cites W2164365774 @default.
- W1983248229 cites W2164478123 @default.
- W1983248229 cites W2164775052 @default.
- W1983248229 cites W2164898121 @default.
- W1983248229 cites W2165302523 @default.
- W1983248229 cites W2167823105 @default.
- W1983248229 cites W2170338752 @default.
- W1983248229 cites W2170868847 @default.
- W1983248229 cites W2171236556 @default.
- W1983248229 doi "https://doi.org/10.1371/journal.ppat.1002197" @default.
- W1983248229 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3164628" @default.