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- W1988497968 abstract "Paracrystalline inclusions formed by influenza A virus nonstructural protein (NS1) in infected BHK21-F cells were found to contain significant amounts of RNA. PAGE analysis and oligonucleotide fingerprint mapping indicated that the inclusion-associated RNA was primarily of cellular origin. Analysis of the RNA by oligodeoxythymidylic acid—cellulose chromatography and in vitro translation indicated that the RNA was not host or viral messenger RNA. Purified inclusions were solubilized with 6 M urea and 1 M KCl, and the NS1 protein was separated from the RNA by sucrose gradient centrifugation. When urea and KCl were removed by dialysis, the NS1 protein reassociated with the RNA. The resulting complexes obtained in the absence of Mg2+ appeared to be smaller and more heterogeneous in size than the original NS, inclusions, whereas complexes obtained in the presence of 10 mM Mg2+ were similar in size to the original NS1 inclusions. RNA-free NS1 protein also formed tightly packed structures similar to the tightly packed NS1-RNA complexes, indicating that RNA is not necessary for this interaction. The buoyant densities in CsCl of the original NS1 inclusions, the reassociated NS1-RNA complexes, and the RNA-free NS1 protein complexes were determined to be 1.347, 1.341, and 1.270 g/ml, respectively. The results indicate that influenza A NS1 protein has binding activity for RNA, and forms inclusions containing bound RNA molecules in the cytoplasm during virus replication." @default.
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- W1988497968 date "1981-04-01" @default.
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- W1988497968 title "Characterization of the rna associated with influenza A cytoplasmic inclusions and the interaction of NS1 protein with RNA" @default.
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- W1988497968 doi "https://doi.org/10.1016/0042-6822(81)90010-6" @default.
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