Matches in SemOpenAlex for { <https://semopenalex.org/work/W1989878327> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1989878327 endingPage "422" @default.
- W1989878327 startingPage "418" @default.
- W1989878327 abstract "Pro-haemolysin (≈110 kDa), the inactive precursor of the membrane-lytic toxin α-haemolysin, has been purified from an overproducing strain of Escherichia coli. Pro-haemolysin forms aggregates in aqueous media, like the mature protein, suggesting an amphipathic structure. Direct measurements of protein binding to liposomal membranes, following a novel procedure, show that pro-haemolysin can bind the lipid bilayers to a similar extent as α-haemolysin. This is confirmed by the observed changes in the intrinsic fluorescence emission of the protein upon binding the bilayers. However, pro-haemolysin is totally unable to induce liposomal membrane lysis. Binding of Ca2+, that is essential for the lytic activity of α-haemolysin, is greatly diminished in the precursor protein, as shown both by direct measurements of 45Ca2+-binding and by fluorescence measurements. The results suggest that binding of a fatty acyl residue in the activation step brings about an important conformation al change in the protein that involves the Ca2+-binding domain." @default.
- W1989878327 created "2016-06-24" @default.
- W1989878327 creator A5017358738 @default.
- W1989878327 creator A5017948814 @default.
- W1989878327 creator A5056505416 @default.
- W1989878327 creator A5078749773 @default.
- W1989878327 date "1996-06-01" @default.
- W1989878327 modified "2023-10-02" @default.
- W1989878327 title "Purification of Escherichia coli Pro-Haemolysin, and a Comparison with the Properties of Mature alpha-haemolysin" @default.
- W1989878327 cites W1513897103 @default.
- W1989878327 cites W1657619266 @default.
- W1989878327 cites W167632067 @default.
- W1989878327 cites W1983483451 @default.
- W1989878327 cites W1994774830 @default.
- W1989878327 cites W2018000738 @default.
- W1989878327 cites W2019358271 @default.
- W1989878327 cites W2032504340 @default.
- W1989878327 cites W2062449665 @default.
- W1989878327 cites W2064735082 @default.
- W1989878327 cites W2079611093 @default.
- W1989878327 cites W2128635872 @default.
- W1989878327 cites W4233308539 @default.
- W1989878327 doi "https://doi.org/10.1111/j.1432-1033.1996.0418z.x" @default.
- W1989878327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8681953" @default.
- W1989878327 hasPublicationYear "1996" @default.
- W1989878327 type Work @default.
- W1989878327 sameAs 1989878327 @default.
- W1989878327 citedByCount "38" @default.
- W1989878327 countsByYear W19898783272012 @default.
- W1989878327 countsByYear W19898783272013 @default.
- W1989878327 countsByYear W19898783272014 @default.
- W1989878327 countsByYear W19898783272015 @default.
- W1989878327 countsByYear W19898783272016 @default.
- W1989878327 countsByYear W19898783272017 @default.
- W1989878327 countsByYear W19898783272018 @default.
- W1989878327 countsByYear W19898783272019 @default.
- W1989878327 countsByYear W19898783272020 @default.
- W1989878327 countsByYear W19898783272022 @default.
- W1989878327 crossrefType "journal-article" @default.
- W1989878327 hasAuthorship W1989878327A5017358738 @default.
- W1989878327 hasAuthorship W1989878327A5017948814 @default.
- W1989878327 hasAuthorship W1989878327A5056505416 @default.
- W1989878327 hasAuthorship W1989878327A5078749773 @default.
- W1989878327 hasBestOaLocation W19898783271 @default.
- W1989878327 hasConcept C104317684 @default.
- W1989878327 hasConcept C12554922 @default.
- W1989878327 hasConcept C136139683 @default.
- W1989878327 hasConcept C159047783 @default.
- W1989878327 hasConcept C182615771 @default.
- W1989878327 hasConcept C185154212 @default.
- W1989878327 hasConcept C185592680 @default.
- W1989878327 hasConcept C2522874641 @default.
- W1989878327 hasConcept C39944091 @default.
- W1989878327 hasConcept C41625074 @default.
- W1989878327 hasConcept C547475151 @default.
- W1989878327 hasConcept C55493867 @default.
- W1989878327 hasConcept C57409179 @default.
- W1989878327 hasConcept C60987743 @default.
- W1989878327 hasConcept C86803240 @default.
- W1989878327 hasConceptScore W1989878327C104317684 @default.
- W1989878327 hasConceptScore W1989878327C12554922 @default.
- W1989878327 hasConceptScore W1989878327C136139683 @default.
- W1989878327 hasConceptScore W1989878327C159047783 @default.
- W1989878327 hasConceptScore W1989878327C182615771 @default.
- W1989878327 hasConceptScore W1989878327C185154212 @default.
- W1989878327 hasConceptScore W1989878327C185592680 @default.
- W1989878327 hasConceptScore W1989878327C2522874641 @default.
- W1989878327 hasConceptScore W1989878327C39944091 @default.
- W1989878327 hasConceptScore W1989878327C41625074 @default.
- W1989878327 hasConceptScore W1989878327C547475151 @default.
- W1989878327 hasConceptScore W1989878327C55493867 @default.
- W1989878327 hasConceptScore W1989878327C57409179 @default.
- W1989878327 hasConceptScore W1989878327C60987743 @default.
- W1989878327 hasConceptScore W1989878327C86803240 @default.
- W1989878327 hasIssue "2" @default.
- W1989878327 hasLocation W19898783271 @default.
- W1989878327 hasLocation W19898783272 @default.
- W1989878327 hasOpenAccess W1989878327 @default.
- W1989878327 hasPrimaryLocation W19898783271 @default.
- W1989878327 hasRelatedWork W1969799452 @default.
- W1989878327 hasRelatedWork W2015968751 @default.
- W1989878327 hasRelatedWork W2017614155 @default.
- W1989878327 hasRelatedWork W2061393157 @default.
- W1989878327 hasRelatedWork W2282678668 @default.
- W1989878327 hasRelatedWork W2742420766 @default.
- W1989878327 hasRelatedWork W3011024476 @default.
- W1989878327 hasRelatedWork W4312396051 @default.
- W1989878327 hasRelatedWork W78021758 @default.
- W1989878327 hasRelatedWork W181634280 @default.
- W1989878327 hasVolume "238" @default.
- W1989878327 isParatext "false" @default.
- W1989878327 isRetracted "false" @default.
- W1989878327 magId "1989878327" @default.
- W1989878327 workType "article" @default.