FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991422670> ?p ?o ?g. }

• W1991422670 endingPage "e53003" @default.
• W1991422670 startingPage "e53003" @default.
• W1991422670 abstract "Human embryonal carcinoma (EC) cells are the stem cells of nonseminoma testicular germ cells tumors (TGCTs) and share remarkable similarities to human embryonic stem (ES) cells. In prior work we found that EC cells are hypersensitive to low nanomolar doses of 5-aza deoxycytidine (5-aza) and that this hypersensitivity partially depended on unusually high levels of the DNA methyltransferase, DNMT3B. We show here that low-dose 5-aza treatment results in DNA damage and induction of p53 in NT2/D1 cells. In addition, low-dose 5-aza results in global and gene specific promoter DNA hypomethylation. Low-dose 5-aza induces a p53 transcriptional signature distinct from that induced with cisplatin in NT2/D1 cells and also uniquely downregulates genes associated with pluripotency including NANOG, SOX2, GDF3 and Myc target genes. Changes in the p53 and pluripotency signatures with 5-aza were to a large extent dependent on high levels of DNMT3B. In contrast to the majority of p53 target genes upregulated by 5-aza that did not show DNA hypomethylation, several other genes induced with 5-aza had corresponding decreases in promoter methylation. These genes include RIN1, SOX15, GPER, and TLR4 and are novel candidate tumors suppressors in TGCTs. Our studies suggest that the hypersensitivity of NT2/D1 cells to low-dose 5-aza is multifactorial and involves the combined activation of p53 targets, repression of pluripotency genes, and activation of genes repressed by DNA methylation. Low-dose 5-aza therapy may be a general strategy to treat those tumors that are sustained by cells with embryonic stem-like properties.GEO NUMBER FOR THE MICROARRAY DATA: GSE42647." @default.
• W1991422670 created "2016-06-24" @default.
• W1991422670 creator A5010680125 @default.
• W1991422670 creator A5011227457 @default.
• W1991422670 creator A5012121180 @default.
• W1991422670 creator A5027854986 @default.
• W1991422670 creator A5045023019 @default.
• W1991422670 creator A5052314508 @default.
• W1991422670 creator A5074762953 @default.
• W1991422670 creator A5081393377 @default.
• W1991422670 date "2012-12-27" @default.
• W1991422670 modified "2023-10-17" @default.
• W1991422670 title "Acute Hypersensitivity of Pluripotent Testicular Cancer-Derived Embryonal Carcinoma to Low-Dose 5-Aza Deoxycytidine Is Associated with Global DNA Damage-Associated p53 Activation, Anti-Pluripotency and DNA Demethylation" @default.
• W1991422670 cites W1591391963 @default.
• W1991422670 cites W1851342022 @default.
• W1991422670 cites W1947495243 @default.
• W1991422670 cites W1966791160 @default.
• W1991422670 cites W1976878409 @default.
• W1991422670 cites W1981394656 @default.
• W1991422670 cites W1982393817 @default.
• W1991422670 cites W1989755555 @default.
• W1991422670 cites W1997638561 @default.
• W1991422670 cites W2003256314 @default.
• W1991422670 cites W2007208661 @default.
• W1991422670 cites W2012400017 @default.
• W1991422670 cites W2013250198 @default.
• W1991422670 cites W2014426141 @default.
• W1991422670 cites W2024088186 @default.
• W1991422670 cites W2027127452 @default.
• W1991422670 cites W2032580087 @default.
• W1991422670 cites W2033963429 @default.
• W1991422670 cites W2039053594 @default.
• W1991422670 cites W2040393829 @default.
• W1991422670 cites W2041686632 @default.
• W1991422670 cites W2045889399 @default.
• W1991422670 cites W2053213384 @default.
• W1991422670 cites W2055169194 @default.
• W1991422670 cites W2055327776 @default.
• W1991422670 cites W2059099579 @default.
• W1991422670 cites W2060660431 @default.
• W1991422670 cites W2061946349 @default.
• W1991422670 cites W2062565707 @default.
• W1991422670 cites W2065751798 @default.
• W1991422670 cites W2070672434 @default.
• W1991422670 cites W2073759231 @default.
• W1991422670 cites W2074765580 @default.
• W1991422670 cites W2076047032 @default.
• W1991422670 cites W2076534093 @default.
• W1991422670 cites W2086328355 @default.
• W1991422670 cites W2104715465 @default.
• W1991422670 cites W2105672290 @default.
• W1991422670 cites W2111920812 @default.
• W1991422670 cites W2112797367 @default.
• W1991422670 cites W2113155233 @default.
• W1991422670 cites W2116824534 @default.
• W1991422670 cites W2122768819 @default.
• W1991422670 cites W2130222411 @default.
• W1991422670 cites W2132478265 @default.
• W1991422670 cites W2136376958 @default.
• W1991422670 cites W2136973375 @default.
• W1991422670 cites W2141476381 @default.
• W1991422670 cites W2144449731 @default.
• W1991422670 cites W2147516356 @default.
• W1991422670 cites W2158668089 @default.
• W1991422670 cites W2160872988 @default.
• W1991422670 cites W2165613749 @default.
• W1991422670 cites W2187482679 @default.
• W1991422670 cites W2313957270 @default.
• W1991422670 doi "https://doi.org/10.1371/journal.pone.0053003" @default.
• W1991422670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3531428" @default.
• W1991422670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23300844" @default.
• W1991422670 hasPublicationYear "2012" @default.
• W1991422670 type Work @default.
• W1991422670 sameAs 1991422670 @default.
• W1991422670 citedByCount "47" @default.
• W1991422670 countsByYear W19914226702013 @default.
• W1991422670 countsByYear W19914226702014 @default.
• W1991422670 countsByYear W19914226702015 @default.
• W1991422670 countsByYear W19914226702016 @default.
• W1991422670 countsByYear W19914226702017 @default.
• W1991422670 countsByYear W19914226702018 @default.
• W1991422670 countsByYear W19914226702019 @default.
• W1991422670 countsByYear W19914226702020 @default.
• W1991422670 countsByYear W19914226702021 @default.
• W1991422670 countsByYear W19914226702022 @default.
• W1991422670 countsByYear W19914226702023 @default.
• W1991422670 crossrefType "journal-article" @default.
• W1991422670 hasAuthorship W1991422670A5010680125 @default.
• W1991422670 hasAuthorship W1991422670A5011227457 @default.
• W1991422670 hasAuthorship W1991422670A5012121180 @default.
• W1991422670 hasAuthorship W1991422670A5027854986 @default.
• W1991422670 hasAuthorship W1991422670A5045023019 @default.
• W1991422670 hasAuthorship W1991422670A5052314508 @default.
• W1991422670 hasAuthorship W1991422670A5074762953 @default.
• W1991422670 hasAuthorship W1991422670A5081393377 @default.
• W1991422670 hasBestOaLocation W19914226701 @default.
• W1991422670 hasConcept C104317684 @default.
• W1991422670 hasConcept C107459253 @default.

• next