FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004126133> ?p ?o ?g. }

• W2004126133 endingPage "68" @default.
• W2004126133 startingPage "61" @default.
• W2004126133 abstract "The genus Metapneumovirus within the subfamily Pneumovirinae and family Paramyxoviridae includes only two viruses, human metapneumovirus (hMPV) and avian metapneumovirus (aMPV), which cause respiratory disease in humans and birds, respectively. These two viruses grow poorly in cell culture and other quantitation methods, such as indirect immuno-staining and immuno-fluorescent assays, are expensive, time consuming, and do not allow for plaque purification of the virus. In order to enhance research efforts for studying these two viruses, a direct plaque assay for both hMPV and aMPV has been developed. By optimizing the chemical components of the agarose overlay, it was found that both hMPV with a trypsin-independent F cleavage site and aMPV formed clear and countable plaques in a number of mammalian cell lines (such as Vero-E6 and LLC-MK2 cells) after 5 days of incubation. The plaque forming assay has similar sensitivity and reliability as the currently used immunological methods for viral quantitation. The plaque assay is also a more simple, rapid, and economical method compared to immunological assays, and in addition allows for plaque purification of the viruses. The direct plaque assay will be a valuable method for the quantitation and evaluation of the biological properties of some metapneumoviruses." @default.
• W2004126133 created "2016-06-24" @default.
• W2004126133 creator A5013944987 @default.
• W2004126133 creator A5050398177 @default.
• W2004126133 creator A5053713168 @default.
• W2004126133 creator A5071773009 @default.
• W2004126133 date "2012-10-01" @default.
• W2004126133 modified "2023-09-23" @default.
• W2004126133 title "Development and optimization of a direct plaque assay for human and avian metapneumoviruses" @default.
• W2004126133 cites W1986110745 @default.
• W2004126133 cites W1994707778 @default.
• W2004126133 cites W1997575588 @default.
• W2004126133 cites W2005329056 @default.
• W2004126133 cites W2022684844 @default.
• W2004126133 cites W2024563102 @default.
• W2004126133 cites W2031507771 @default.
• W2004126133 cites W2042556359 @default.
• W2004126133 cites W2048618475 @default.
• W2004126133 cites W2071301221 @default.
• W2004126133 cites W2076462632 @default.
• W2004126133 cites W2104915849 @default.
• W2004126133 cites W2114064040 @default.
• W2004126133 cites W2119681257 @default.
• W2004126133 cites W2121967038 @default.
• W2004126133 cites W2128788856 @default.
• W2004126133 cites W2135259291 @default.
• W2004126133 cites W2161288030 @default.
• W2004126133 cites W2163537732 @default.
• W2004126133 cites W2165127900 @default.
• W2004126133 cites W2168252145 @default.
• W2004126133 cites W2170380549 @default.
• W2004126133 cites W2170881661 @default.
• W2004126133 cites W2171602693 @default.
• W2004126133 cites W2172607546 @default.
• W2004126133 cites W2256756668 @default.
• W2004126133 cites W2318915316 @default.
• W2004126133 doi "https://doi.org/10.1016/j.jviromet.2012.05.030" @default.
• W2004126133 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3811939" @default.
• W2004126133 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22684013" @default.
• W2004126133 hasPublicationYear "2012" @default.
• W2004126133 type Work @default.
• W2004126133 sameAs 2004126133 @default.
• W2004126133 citedByCount "21" @default.
• W2004126133 countsByYear W20041261332014 @default.
• W2004126133 countsByYear W20041261332015 @default.
• W2004126133 countsByYear W20041261332016 @default.
• W2004126133 countsByYear W20041261332017 @default.
• W2004126133 countsByYear W20041261332018 @default.
• W2004126133 countsByYear W20041261332019 @default.
• W2004126133 countsByYear W20041261332020 @default.
• W2004126133 countsByYear W20041261332021 @default.
• W2004126133 countsByYear W20041261332022 @default.
• W2004126133 crossrefType "journal-article" @default.
• W2004126133 hasAuthorship W2004126133A5013944987 @default.
• W2004126133 hasAuthorship W2004126133A5050398177 @default.
• W2004126133 hasAuthorship W2004126133A5053713168 @default.
• W2004126133 hasAuthorship W2004126133A5071773009 @default.
• W2004126133 hasBestOaLocation W20041261332 @default.
• W2004126133 hasConcept C105702510 @default.
• W2004126133 hasConcept C153911025 @default.
• W2004126133 hasConcept C159047783 @default.
• W2004126133 hasConcept C2522874641 @default.
• W2004126133 hasConcept C2776012195 @default.
• W2004126133 hasConcept C2778476535 @default.
• W2004126133 hasConcept C2780355102 @default.
• W2004126133 hasConcept C2780475896 @default.
• W2004126133 hasConcept C2780727368 @default.
• W2004126133 hasConcept C2780813555 @default.
• W2004126133 hasConcept C2911218186 @default.
• W2004126133 hasConcept C534529494 @default.
• W2004126133 hasConcept C70913881 @default.
• W2004126133 hasConcept C86803240 @default.
• W2004126133 hasConcept C89423630 @default.
• W2004126133 hasConceptScore W2004126133C105702510 @default.
• W2004126133 hasConceptScore W2004126133C153911025 @default.
• W2004126133 hasConceptScore W2004126133C159047783 @default.
• W2004126133 hasConceptScore W2004126133C2522874641 @default.
• W2004126133 hasConceptScore W2004126133C2776012195 @default.
• W2004126133 hasConceptScore W2004126133C2778476535 @default.
• W2004126133 hasConceptScore W2004126133C2780355102 @default.
• W2004126133 hasConceptScore W2004126133C2780475896 @default.
• W2004126133 hasConceptScore W2004126133C2780727368 @default.
• W2004126133 hasConceptScore W2004126133C2780813555 @default.
• W2004126133 hasConceptScore W2004126133C2911218186 @default.
• W2004126133 hasConceptScore W2004126133C534529494 @default.
• W2004126133 hasConceptScore W2004126133C70913881 @default.
• W2004126133 hasConceptScore W2004126133C86803240 @default.
• W2004126133 hasConceptScore W2004126133C89423630 @default.
• W2004126133 hasIssue "1" @default.
• W2004126133 hasLocation W20041261331 @default.
• W2004126133 hasLocation W20041261332 @default.
• W2004126133 hasLocation W20041261333 @default.
• W2004126133 hasLocation W20041261334 @default.
• W2004126133 hasOpenAccess W2004126133 @default.
• W2004126133 hasPrimaryLocation W20041261331 @default.
• W2004126133 hasRelatedWork W1485814823 @default.
• W2004126133 hasRelatedWork W2004126133 @default.
• W2004126133 hasRelatedWork W2068567885 @default.

• next