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• W2006464504 abstract "Gastric cancer is an important cause of cancer-related mortality worldwide (1). There is increasing evidence that the existence of cancer stem cells (CSC) is responsible for tumour formation and maintenance.The present study was designed to recognise circulating CSCs from blood samples of patients with gastric cancer, using CD133 and ABCG2 as potential markers. CD133(-) , CD133(+) ABCG2(-) and CD133(+) ABCG2(+) cells lines were analysed by flow cytometry, immunofluorescence staining, western blotting and real-time PCR. Furthermore, functional assays (clonogenic assay in vitro and tumourigenic assay in vivo) were also performed using these cell lines.Higher percentages of CD133(+) cells were identified in blood samples from gastric cancer patients compared to normal controls. In addition, we found by using Kaplan-Meier analysis, that numbers of CD133(+) cells correlated with poor prognosis gastric cancer patients. Finally, tumourigenic properties of CD133(+) ABCG2(+) cells were determined in vitro and in vivo.Our in vitro and in vivo experiments demonstrated that CD133(+) ABCG2(+) cells exhibited well-known CSC characteristics; thus when circulating they could be used as a prognostic marker for gastric cancer." @default.
• W2006464504 created "2016-06-24" @default.
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• W2006464504 date "2015-02-27" @default.
• W2006464504 modified "2023-10-12" @default.
• W2006464504 title "Prognostic value of circulating CD133⁺cells in patients with gastric cancer" @default.
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• W2006464504 doi "https://doi.org/10.1111/cpr.12175" @default.
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