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- W2006829501 abstract "Here we describe a technique for studying hippocampal postnatal neurogenesis in the rodent brain using the organotypic slice culture technique. This method maintains the characteristic topographical morphology of the hippocampus while allowing direct application of pharmacological agents to the developing hippocampal dentate gyrus. Additionally, slice cultures can be maintained for up to 4 weeks and thus, allow one to study the maturation process of newborn granule neurons. Slice cultures allow for efficient pharmacological manipulation of hippocampal slices while excluding complex variables such as uncertainties related to the deep anatomic location of the hippocampus as well as the blood brain barrier. For these reasons, we sought to optimize organotypic slice cultures specifically for postnatal neurogenesis research." @default.
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- W2006829501 date "2015-03-04" @default.
- W2006829501 modified "2023-09-27" @default.
- W2006829501 title "Organotypic Slice Cultures for Studies of Postnatal Neurogenesis" @default.
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- W2006829501 doi "https://doi.org/10.3791/52353" @default.
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