FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2009554993> ?p ?o ?g. }

• W2009554993 endingPage "8" @default.
• W2009554993 startingPage "1" @default.
• W2009554993 abstract "Although protein prenylation is widely studied, there are few good methods for isolating prenylated proteins from their nonprenylated relatives. We report that crosslinked agarose (e.g., Sepharose) chromatography medium that has been chemically functionalized with beta-cyclodextrin (beta-CD) is extremely effective in affinity chromatography of prenylated proteins. In this study, a variety of proteins with C-terminal prenylation target (CAAX box) sequences were enzymatically prenylated in vitro with natural and nonnatural prenyl diphosphate substrates. The prenylated protein products could then be isolated from starting materials by gravity chromatography or fast protein liquid chromatography (FPLC) on a beta-CD-Sepharose column. One particular prenylation reaction, farnesylation of an mCherry-CAAX fusion construct, was studied in detail. In this case, purified farnesylated product was unambiguously identified by electrospray mass spectrometry. In addition, when mCherry-CAAX was prenylated with a nonnatural, functional isoprenoid substrate, the functional group was maintained by chromatography on beta-CD-Sepharose, such that the resulting protein could be selectively bound at its C terminus to complementary functionality on a solid substrate. Finally, beta-CD-Sepharose FPLC was used to isolate prenylated mCherry-CAAX from crude HeLa cell lysate as a model for purifying prenylated proteins from cell extracts. We propose that this method could be generally useful to the community of researchers studying protein prenylation." @default.
• W2009554993 created "2016-06-24" @default.
• W2009554993 creator A5013100170 @default.
• W2009554993 creator A5014973540 @default.
• W2009554993 creator A5016360627 @default.
• W2009554993 creator A5023693129 @default.
• W2009554993 creator A5043458102 @default.
• W2009554993 creator A5048332389 @default.
• W2009554993 creator A5057734727 @default.
• W2009554993 creator A5090030371 @default.
• W2009554993 date "2009-03-01" @default.
• W2009554993 modified "2023-09-24" @default.
• W2009554993 title "Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose" @default.
• W2009554993 cites W1949772429 @default.
• W2009554993 cites W1964980219 @default.
• W2009554993 cites W1966112545 @default.
• W2009554993 cites W1975360275 @default.
• W2009554993 cites W1977104401 @default.
• W2009554993 cites W1978392336 @default.
• W2009554993 cites W1984036493 @default.
• W2009554993 cites W1990524685 @default.
• W2009554993 cites W2013285339 @default.
• W2009554993 cites W2013608848 @default.
• W2009554993 cites W2018934684 @default.
• W2009554993 cites W2021320094 @default.
• W2009554993 cites W2022454349 @default.
• W2009554993 cites W2023433298 @default.
• W2009554993 cites W2024166317 @default.
• W2009554993 cites W2024894588 @default.
• W2009554993 cites W2027530605 @default.
• W2009554993 cites W2031079064 @default.
• W2009554993 cites W2035654066 @default.
• W2009554993 cites W2038372594 @default.
• W2009554993 cites W2040171181 @default.
• W2009554993 cites W2050367572 @default.
• W2009554993 cites W2052344045 @default.
• W2009554993 cites W2052731133 @default.
• W2009554993 cites W2052843426 @default.
• W2009554993 cites W2054498733 @default.
• W2009554993 cites W2054771288 @default.
• W2009554993 cites W2055450030 @default.
• W2009554993 cites W2061216289 @default.
• W2009554993 cites W2061302578 @default.
• W2009554993 cites W2065507415 @default.
• W2009554993 cites W2067100185 @default.
• W2009554993 cites W2078086283 @default.
• W2009554993 cites W2079583019 @default.
• W2009554993 cites W2089906643 @default.
• W2009554993 cites W2090533815 @default.
• W2009554993 cites W2096952782 @default.
• W2009554993 cites W2098681139 @default.
• W2009554993 cites W2100628634 @default.
• W2009554993 cites W2115734610 @default.
• W2009554993 cites W2117876468 @default.
• W2009554993 cites W2128392882 @default.
• W2009554993 cites W2140118120 @default.
• W2009554993 cites W2163618462 @default.
• W2009554993 cites W2176744910 @default.
• W2009554993 cites W2218331703 @default.
• W2009554993 cites W2408045763 @default.
• W2009554993 cites W4256503849 @default.
• W2009554993 doi "https://doi.org/10.1016/j.ab.2008.09.007" @default.
• W2009554993 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2654400" @default.
• W2009554993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18834849" @default.
• W2009554993 hasPublicationYear "2009" @default.
• W2009554993 type Work @default.
• W2009554993 sameAs 2009554993 @default.
• W2009554993 citedByCount "19" @default.
• W2009554993 countsByYear W20095549932012 @default.
• W2009554993 countsByYear W20095549932013 @default.
• W2009554993 countsByYear W20095549932015 @default.
• W2009554993 countsByYear W20095549932016 @default.
• W2009554993 countsByYear W20095549932018 @default.
• W2009554993 countsByYear W20095549932020 @default.
• W2009554993 crossrefType "journal-article" @default.
• W2009554993 hasAuthorship W2009554993A5013100170 @default.
• W2009554993 hasAuthorship W2009554993A5014973540 @default.
• W2009554993 hasAuthorship W2009554993A5016360627 @default.
• W2009554993 hasAuthorship W2009554993A5023693129 @default.
• W2009554993 hasAuthorship W2009554993A5043458102 @default.
• W2009554993 hasAuthorship W2009554993A5048332389 @default.
• W2009554993 hasAuthorship W2009554993A5057734727 @default.
• W2009554993 hasAuthorship W2009554993A5090030371 @default.
• W2009554993 hasBestOaLocation W20095549932 @default.
• W2009554993 hasConcept C116817701 @default.
• W2009554993 hasConcept C179247698 @default.
• W2009554993 hasConcept C179998833 @default.
• W2009554993 hasConcept C181199279 @default.
• W2009554993 hasConcept C185592680 @default.
• W2009554993 hasConcept C195310446 @default.
• W2009554993 hasConcept C2779881004 @default.
• W2009554993 hasConcept C43617362 @default.
• W2009554993 hasConcept C55493867 @default.
• W2009554993 hasConcept C57790582 @default.
• W2009554993 hasConceptScore W2009554993C116817701 @default.
• W2009554993 hasConceptScore W2009554993C179247698 @default.
• W2009554993 hasConceptScore W2009554993C179998833 @default.
• W2009554993 hasConceptScore W2009554993C181199279 @default.

• next