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• W2013940033 abstract "Karyotype is a well established technique in the study of spontaneous miscarriages but is associated with selective overgrowth of maternal cells and other culture artefact (spp) such as tetraploidy, which could mask the true karyotype of the conceptus. 328 cases of pregnancy losses were studied by karyotype and Multiplex Ligation Dependent Probe Amplification technique. Touch FISH performed in non-cultured cells was used to evaluate the ploidy complement and sex discrepancies using centromeric probes for chromosomes X, Y and 18. Touch FISH confirmed 13 cases of maternal contamination, identified a triploidy and a monosomy X. True tetraploidy was confirmed in 7/14 cases studied. Touch FISH protocol is extremely accurate in the distinction of genuine mosaicism from tissue culture artifacts namely in cases with suspicion of tetraploidy and can be used to confirm maternal cell contamination in cases with sex discrepancy between karyotype and MLPA." @default.
• W2013940033 created "2016-06-24" @default.
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• W2013940033 date "2010-07-31" @default.
• W2013940033 modified "2023-10-17" @default.
• W2013940033 title "Application of touch FISH in the study of mosaic tetraploidy and maternal cell contamination in pregnancy losses" @default.
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• W2013940033 doi "https://doi.org/10.1007/s10815-010-9460-1" @default.
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