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- W2024182636 abstract "The influence of assay variation and duration of storage on changes in cerebrospinal fluid (CSF) levels of tau and phosphorylated (P)-tau with time was evaluated in 112 patients with various neurological disorders.These patients (aged 66+/-9 years, 52% male), referred to our memory clinic, underwent two spinal taps (mean interval 19 months) and the baseline samples were assayed twice in a sandwich enzyme-linked immunosorbent assay (ELISA): once after the first spinal tap (A1) and once in a separately stored aliquot (A2) simultaneous with the follow-up sample (B).Coefficients of variances (CVs) of tau and P-tau levels determined in repeated spinal taps (DeltaB-A2) measured in one assay (10.9% and 7.6%) were lower (p<0.01) than the CVs observed in two different (DeltaB-A1) assays (16.5% and 11.7%). The CVs of tau and P-tau measurement of one CSF sample repeated on two occasions (DeltaA1-A2) were 12.3% and 8.6%. A difference in mean P-tau level was found if the same CSF samples were repeatedly measured in two different ELISAs (A1-A2).Longitudinal CSF tau and P-tau are best measured in one assay resulting in a lower variability compared to measurement in two different assays. The within person variability in levels of these markers currently limits the use of these ELISAs in a longitudinal clinical setting." @default.
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- W2024182636 date "2008-01-01" @default.
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- W2024182636 title "Variability in longitudinal cerebrospinal fluid tau and phosphorylated tau measurements" @default.
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- W2024182636 doi "https://doi.org/10.1515/cclm.2008.241" @default.
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