Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035145730> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2035145730 endingPage "259" @default.
- W2035145730 startingPage "253" @default.
- W2035145730 abstract "The immobilisation of enzymes is important for applications in bioelectrochemistry such as biosensors or biofuel cells. In this paper we report the immobilisation of lactate dehydrogenase (LDH) on poly(aniline)–poly(acrylate) [PANi–PAA] and poly(aniline)–poly(vinylsulfonate) [PANi–PVS] composite films. Two genetically engineered forms of LDH (E.C.1.1.1.27) from Bacillus stearothermophilus, one with a poly(histidine) tag on the C-Terminus (LDH-CHis) the other with a poly(histidine) tag on the N-terminus (LDH-NHis), together with the wild type enzyme (WT-LDH) were studied. The LDH-CHis and LDH-NHis both have better affinity for the poly(aniline)–poly(anion) composite films than the WT-LDH. The immobilised LDH reduces the coenzyme NAD+ to NADH and oxidises the substrate, l-lactate, to pyruvate. The NADH produced is then oxidised at the poly(aniline)–poly(anion) composite films. The effects of buffer concentration, temperature, NAD+ concentration, enzyme immobilisation conditions, film thickness and electrode rotation rate on the catalytic current were all investigated." @default.
- W2035145730 created "2016-06-24" @default.
- W2035145730 creator A5011668039 @default.
- W2035145730 creator A5027301994 @default.
- W2035145730 creator A5035392287 @default.
- W2035145730 creator A5089215572 @default.
- W2035145730 creator A5091773566 @default.
- W2035145730 date "2002-12-01" @default.
- W2035145730 modified "2023-10-16" @default.
- W2035145730 title "Oxidation of NADH produced by a lactate dehydrogenase immobilised on poly(aniline)–poly(anion) composite films" @default.
- W2035145730 cites W119933002 @default.
- W2035145730 cites W1966403262 @default.
- W2035145730 cites W1984909861 @default.
- W2035145730 cites W1999034913 @default.
- W2035145730 cites W2015145241 @default.
- W2035145730 cites W2019356361 @default.
- W2035145730 cites W2023811962 @default.
- W2035145730 cites W2043727313 @default.
- W2035145730 cites W2047969498 @default.
- W2035145730 cites W2068932458 @default.
- W2035145730 cites W2099443896 @default.
- W2035145730 doi "https://doi.org/10.1016/s0022-0728(02)00833-1" @default.
- W2035145730 hasPublicationYear "2002" @default.
- W2035145730 type Work @default.
- W2035145730 sameAs 2035145730 @default.
- W2035145730 citedByCount "23" @default.
- W2035145730 countsByYear W20351457302012 @default.
- W2035145730 countsByYear W20351457302015 @default.
- W2035145730 countsByYear W20351457302016 @default.
- W2035145730 countsByYear W20351457302017 @default.
- W2035145730 countsByYear W20351457302018 @default.
- W2035145730 crossrefType "journal-article" @default.
- W2035145730 hasAuthorship W2035145730A5011668039 @default.
- W2035145730 hasAuthorship W2035145730A5027301994 @default.
- W2035145730 hasAuthorship W2035145730A5035392287 @default.
- W2035145730 hasAuthorship W2035145730A5089215572 @default.
- W2035145730 hasAuthorship W2035145730A5091773566 @default.
- W2035145730 hasConcept C111368507 @default.
- W2035145730 hasConcept C127313418 @default.
- W2035145730 hasConcept C13965031 @default.
- W2035145730 hasConcept C160756335 @default.
- W2035145730 hasConcept C178790620 @default.
- W2035145730 hasConcept C181199279 @default.
- W2035145730 hasConcept C185592680 @default.
- W2035145730 hasConcept C188027245 @default.
- W2035145730 hasConcept C2776317432 @default.
- W2035145730 hasConcept C2777289219 @default.
- W2035145730 hasConcept C2777318727 @default.
- W2035145730 hasConcept C2778460671 @default.
- W2035145730 hasConcept C2779244968 @default.
- W2035145730 hasConcept C33633552 @default.
- W2035145730 hasConcept C55493867 @default.
- W2035145730 hasConcept C75520062 @default.
- W2035145730 hasConceptScore W2035145730C111368507 @default.
- W2035145730 hasConceptScore W2035145730C127313418 @default.
- W2035145730 hasConceptScore W2035145730C13965031 @default.
- W2035145730 hasConceptScore W2035145730C160756335 @default.
- W2035145730 hasConceptScore W2035145730C178790620 @default.
- W2035145730 hasConceptScore W2035145730C181199279 @default.
- W2035145730 hasConceptScore W2035145730C185592680 @default.
- W2035145730 hasConceptScore W2035145730C188027245 @default.
- W2035145730 hasConceptScore W2035145730C2776317432 @default.
- W2035145730 hasConceptScore W2035145730C2777289219 @default.
- W2035145730 hasConceptScore W2035145730C2777318727 @default.
- W2035145730 hasConceptScore W2035145730C2778460671 @default.
- W2035145730 hasConceptScore W2035145730C2779244968 @default.
- W2035145730 hasConceptScore W2035145730C33633552 @default.
- W2035145730 hasConceptScore W2035145730C55493867 @default.
- W2035145730 hasConceptScore W2035145730C75520062 @default.
- W2035145730 hasLocation W20351457301 @default.
- W2035145730 hasOpenAccess W2035145730 @default.
- W2035145730 hasPrimaryLocation W20351457301 @default.
- W2035145730 hasRelatedWork W1965490062 @default.
- W2035145730 hasRelatedWork W1989056897 @default.
- W2035145730 hasRelatedWork W2012199819 @default.
- W2035145730 hasRelatedWork W2012944759 @default.
- W2035145730 hasRelatedWork W2017557442 @default.
- W2035145730 hasRelatedWork W2029442556 @default.
- W2035145730 hasRelatedWork W2076799412 @default.
- W2035145730 hasRelatedWork W2095245308 @default.
- W2035145730 hasRelatedWork W2121207581 @default.
- W2035145730 hasRelatedWork W2132473245 @default.
- W2035145730 hasVolume "538-539" @default.
- W2035145730 isParatext "false" @default.
- W2035145730 isRetracted "false" @default.
- W2035145730 magId "2035145730" @default.
- W2035145730 workType "article" @default.