FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035891906> ?p ?o ?g. }

• W2035891906 endingPage "656" @default.
• W2035891906 startingPage "648" @default.
• W2035891906 abstract "Insect prophenoloxidases (PPOs) are a group of important innate immunity proteins. Although there have been numerous studies dealing with the PPO activation cascade, the detailed biochemical behaviors of the PPO family proteins remain to be clearly established. This is due primarily to the difficulty in obtaining adequate amounts of PPO proteins for comprehensive characterization. In this study, we expressed three Drosophila melanogaster PPO genes in Escherichia coli, and extensively evaluated expression conditions for obtaining soluble proteins. Through the manipulation of expression conditions, particularly the culture temperature of PPO-transformed E. coli cells, we were able to obtain large quantities of soluble recombinant PPO proteins. Additional Cu2+, either added into the culture medium during PPO induction or directly mixed with the purified rPPO preparations, was necessary to produce Cu2+ associated proenzymes. Cu2+ associated PPOs showed obvious enzyme activities after activation by either ethanol or cetylpyridinium chloride, or by AMM1 (a pupal protein fraction containing native serine proteases for PPO activation). Dose responses for association of individual purified Drosophila rPPOs with Cu2+ showed that Drosophila rPPO1 and rPPO3 had relatively higher affinity for Cu2+ than rPPO2 did. Surprisingly, however, high concentration of Cu2+ (2 mM) completely inhibited PPO activity. Each rPPO had similar activity when dopamine or l-DOPA was the substrate. However, rPPO1 alone had very high activity if l-tyrosine was used as a substrate. After activation by ethanol or 2-propanol, Km and Vmax of the three rPPOs changed as shown in the following: rPPO2 < rPPO3 < rPPO1. If activated by ethanol, the Km and Vmax of each rPPO were lower than by 2-propanol. Due to the difficulty in obtaining functional PPOs via traditional purification methods, the method established in this study will be helpful to produce active insect recombinant PPOs for the study of PPO properties and functions in the future." @default.
• W2035891906 created "2016-06-24" @default.
• W2035891906 creator A5003459068 @default.
• W2035891906 creator A5004905770 @default.
• W2035891906 creator A5015736102 @default.
• W2035891906 creator A5016485135 @default.
• W2035891906 creator A5028012364 @default.
• W2035891906 creator A5040795581 @default.
• W2035891906 creator A5048279362 @default.
• W2035891906 creator A5048564689 @default.
• W2035891906 creator A5073776679 @default.
• W2035891906 creator A5077274035 @default.
• W2035891906 creator A5084398408 @default.
• W2035891906 date "2012-04-01" @default.
• W2035891906 modified "2023-10-17" @default.
• W2035891906 title "Properties of Drosophila melanogaster prophenoloxidases expressed in Escherichia coli" @default.
• W2035891906 cites W101295810 @default.
• W2035891906 cites W1964047219 @default.
• W2035891906 cites W1969157447 @default.
• W2035891906 cites W1977891171 @default.
• W2035891906 cites W1981440814 @default.
• W2035891906 cites W2002793735 @default.
• W2035891906 cites W2004538426 @default.
• W2035891906 cites W2008586137 @default.
• W2035891906 cites W2015446823 @default.
• W2035891906 cites W2018412254 @default.
• W2035891906 cites W2024134510 @default.
• W2035891906 cites W2031865848 @default.
• W2035891906 cites W2036302840 @default.
• W2035891906 cites W2040007543 @default.
• W2035891906 cites W2055490715 @default.
• W2035891906 cites W2059237201 @default.
• W2035891906 cites W2077586513 @default.
• W2035891906 cites W2088245795 @default.
• W2035891906 cites W2102117291 @default.
• W2035891906 cites W2102517829 @default.
• W2035891906 cites W2127983416 @default.
• W2035891906 cites W2139400351 @default.
• W2035891906 cites W2147853830 @default.
• W2035891906 cites W223852488 @default.
• W2035891906 cites W263115363 @default.
• W2035891906 doi "https://doi.org/10.1016/j.dci.2011.11.005" @default.
• W2035891906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22120533" @default.
• W2035891906 hasPublicationYear "2012" @default.
• W2035891906 type Work @default.
• W2035891906 sameAs 2035891906 @default.
• W2035891906 citedByCount "42" @default.
• W2035891906 countsByYear W20358919062012 @default.
• W2035891906 countsByYear W20358919062013 @default.
• W2035891906 countsByYear W20358919062014 @default.
• W2035891906 countsByYear W20358919062015 @default.
• W2035891906 countsByYear W20358919062016 @default.
• W2035891906 countsByYear W20358919062017 @default.
• W2035891906 countsByYear W20358919062018 @default.
• W2035891906 countsByYear W20358919062020 @default.
• W2035891906 countsByYear W20358919062021 @default.
• W2035891906 countsByYear W20358919062022 @default.
• W2035891906 countsByYear W20358919062023 @default.
• W2035891906 crossrefType "journal-article" @default.
• W2035891906 hasAuthorship W2035891906A5003459068 @default.
• W2035891906 hasAuthorship W2035891906A5004905770 @default.
• W2035891906 hasAuthorship W2035891906A5015736102 @default.
• W2035891906 hasAuthorship W2035891906A5016485135 @default.
• W2035891906 hasAuthorship W2035891906A5028012364 @default.
• W2035891906 hasAuthorship W2035891906A5040795581 @default.
• W2035891906 hasAuthorship W2035891906A5048279362 @default.
• W2035891906 hasAuthorship W2035891906A5048564689 @default.
• W2035891906 hasAuthorship W2035891906A5073776679 @default.
• W2035891906 hasAuthorship W2035891906A5077274035 @default.
• W2035891906 hasAuthorship W2035891906A5084398408 @default.
• W2035891906 hasConcept C104317684 @default.
• W2035891906 hasConcept C153911025 @default.
• W2035891906 hasConcept C181199279 @default.
• W2035891906 hasConcept C182220744 @default.
• W2035891906 hasConcept C198684833 @default.
• W2035891906 hasConcept C2776142715 @default.
• W2035891906 hasConcept C2776414213 @default.
• W2035891906 hasConcept C2780104201 @default.
• W2035891906 hasConcept C2780161600 @default.
• W2035891906 hasConcept C547475151 @default.
• W2035891906 hasConcept C55493867 @default.
• W2035891906 hasConcept C86803240 @default.
• W2035891906 hasConceptScore W2035891906C104317684 @default.
• W2035891906 hasConceptScore W2035891906C153911025 @default.
• W2035891906 hasConceptScore W2035891906C181199279 @default.
• W2035891906 hasConceptScore W2035891906C182220744 @default.
• W2035891906 hasConceptScore W2035891906C198684833 @default.
• W2035891906 hasConceptScore W2035891906C2776142715 @default.
• W2035891906 hasConceptScore W2035891906C2776414213 @default.
• W2035891906 hasConceptScore W2035891906C2780104201 @default.
• W2035891906 hasConceptScore W2035891906C2780161600 @default.
• W2035891906 hasConceptScore W2035891906C547475151 @default.
• W2035891906 hasConceptScore W2035891906C55493867 @default.
• W2035891906 hasConceptScore W2035891906C86803240 @default.
• W2035891906 hasFunder F4320321001 @default.
• W2035891906 hasIssue "4" @default.
• W2035891906 hasLocation W20358919061 @default.
• W2035891906 hasLocation W20358919062 @default.

• next