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- W2041635703 abstract "Improved procedures for guanidination of lysine-containing peptides, a derivatization that results in increased MALDI mass spectral signal intensities are presented. The complete conversion of lysines to homoarginines can be accomplished in as little as 5 min. The method is demonstrated on a model peptide and on tryptic digests of three proteins. To demonstrate the applicability to proteomics samples, it is successfully applied to the digest of 50 fmol of a protein. Approaches for concentrating and purifying low-quantity protein digests following guanidination are evaluated. Experiments with the model peptide GRGDSPK enable investigation of the specificity of the guanidination reaction." @default.
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- W2041635703 date "2002-03-13" @default.
- W2041635703 modified "2023-10-17" @default.
- W2041635703 title "Optimization of Guanidination Procedures for MALDI Mass Mapping" @default.
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- W2041635703 doi "https://doi.org/10.1021/ac015613o" @default.
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