FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044858025> ?p ?o ?g. }

• W2044858025 endingPage "150" @default.
• W2044858025 startingPage "141" @default.
• W2044858025 abstract "Background: The kinetochore attachment (spindle assembly) checkpoint arrests cells in metaphase to prevent exit from mitosis until all the chromosomes are aligned properly at the metaphase plate. The checkpoint operates by preventing activation of the anaphase-promoting complex (APC), which triggers anaphase by degrading mitotic cyclins and other proteins. This checkpoint is active during normal mitosis and upon experimental disruption of the mitotic spindle. In yeast, the serine/threonine protein kinase Bub1 and the WD-repeat protein Bub3 are elements of a signal transduction cascade that regulates the kinetochore attachment checkpoint. In mammalian cells, activated MAPK is present on kinetochores during mitosis and activity is upregulated by the spindle assembly checkpoint. In vertebrate unfertilized eggs, a special form of meiotic metaphase arrest by cytostatic factor (CSF) is mediated by MAPK activation of the protein kinase p90Rsk, which leads to inhibition of the APC. However, it is not known whether CSF-dependent metaphase arrest caused by p90Rsk involves components of the spindle assembly checkpoint.Results: xBub1 is present in resting oocytes and its protein level increases slightly during oocyte maturation and early embryogenesis. In Xenopus oocytes, Bub1 is localized to kinetochores during both meiosis I and meiosis II, and the electrophoretic mobility of Bub1 upon SDS-PAGE decreases during meiosis I, reflecting phosphorylation and activation of the enzyme. The activation of Bub1 can be induced in interphase egg extracts by selective stimulation of the MAPK pathway by c-Mos, a MAPKKK. In oocytes treated with the MEK1 inhibitor U0126, the MAPK pathway does not become activated, and Bub1 remains in its low-activity, unshifted form. Injection of a constitutively active target of MAPK, the protein kinase p90Rsk, restores the activation of Bub1 in the presence of U0126. Moreover, purified p90Rsk phosphorylates Bub1 in vitro and increases its protein kinase activity.Conclusions: Bub1, an upstream component of the kinetochore attachment checkpoint, is activated during meiosis in Xenopus in a MAPK-dependent manner. Moreover, a single substrate of MAPK, p90Rsk, is sufficient to activate Bub1 in vitro and in vivo. These results indicate that in vertebrate eggs, kinetochore attachment/spindle assembly checkpoint proteins, including Bub1, are downstream of p90Rsk and may be effectors of APC inhibition and CSF-dependent metaphase arrest by p90Rsk." @default.
• W2044858025 created "2016-06-24" @default.
• W2044858025 creator A5020001016 @default.
• W2044858025 creator A5020952915 @default.
• W2044858025 creator A5027017988 @default.
• W2044858025 creator A5035705491 @default.
• W2044858025 creator A5037289060 @default.
• W2044858025 creator A5066035786 @default.
• W2044858025 creator A5077008742 @default.
• W2044858025 date "2001-02-01" @default.
• W2044858025 modified "2023-09-25" @default.
• W2044858025 title "Bub1 is activated by the protein kinase p90 Rsk during Xenopus oocyte maturation" @default.
• W2044858025 cites W1488282372 @default.
• W2044858025 cites W1537737236 @default.
• W2044858025 cites W1586171740 @default.
• W2044858025 cites W1871272574 @default.
• W2044858025 cites W1963750681 @default.
• W2044858025 cites W1967100183 @default.
• W2044858025 cites W1968174450 @default.
• W2044858025 cites W1972255099 @default.
• W2044858025 cites W1973916918 @default.
• W2044858025 cites W1975058628 @default.
• W2044858025 cites W1975072760 @default.
• W2044858025 cites W1987246052 @default.
• W2044858025 cites W1990637592 @default.
• W2044858025 cites W1994177513 @default.
• W2044858025 cites W2011410033 @default.
• W2044858025 cites W2013801092 @default.
• W2044858025 cites W2015851881 @default.
• W2044858025 cites W2016019304 @default.
• W2044858025 cites W2018711267 @default.
• W2044858025 cites W2020099509 @default.
• W2044858025 cites W2027766408 @default.
• W2044858025 cites W2032413267 @default.
• W2044858025 cites W2032422144 @default.
• W2044858025 cites W2033141310 @default.
• W2044858025 cites W2055895914 @default.
• W2044858025 cites W2058653019 @default.
• W2044858025 cites W2059623287 @default.
• W2044858025 cites W2071204781 @default.
• W2044858025 cites W2073239182 @default.
• W2044858025 cites W2077278757 @default.
• W2044858025 cites W2080628130 @default.
• W2044858025 cites W2081128958 @default.
• W2044858025 cites W2086262311 @default.
• W2044858025 cites W2097973787 @default.
• W2044858025 cites W2104764445 @default.
• W2044858025 cites W2106285650 @default.
• W2044858025 cites W2111069037 @default.
• W2044858025 cites W2119989860 @default.
• W2044858025 cites W2126683450 @default.
• W2044858025 cites W2128973947 @default.
• W2044858025 cites W2129153399 @default.
• W2044858025 cites W2136630749 @default.
• W2044858025 cites W2138817040 @default.
• W2044858025 cites W2147600141 @default.
• W2044858025 cites W2154241215 @default.
• W2044858025 cites W2172240299 @default.
• W2044858025 cites W2186190038 @default.
• W2044858025 cites W935855719 @default.
• W2044858025 cites W93901503 @default.
• W2044858025 doi "https://doi.org/10.1016/s0960-9822(01)00045-8" @default.
• W2044858025 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11231148" @default.
• W2044858025 hasPublicationYear "2001" @default.
• W2044858025 type Work @default.
• W2044858025 sameAs 2044858025 @default.
• W2044858025 citedByCount "98" @default.
• W2044858025 countsByYear W20448580252012 @default.
• W2044858025 countsByYear W20448580252013 @default.
• W2044858025 countsByYear W20448580252014 @default.
• W2044858025 countsByYear W20448580252016 @default.
• W2044858025 countsByYear W20448580252017 @default.
• W2044858025 countsByYear W20448580252018 @default.
• W2044858025 countsByYear W20448580252020 @default.
• W2044858025 crossrefType "journal-article" @default.
• W2044858025 hasAuthorship W2044858025A5020001016 @default.
• W2044858025 hasAuthorship W2044858025A5020952915 @default.
• W2044858025 hasAuthorship W2044858025A5027017988 @default.
• W2044858025 hasAuthorship W2044858025A5035705491 @default.
• W2044858025 hasAuthorship W2044858025A5037289060 @default.
• W2044858025 hasAuthorship W2044858025A5066035786 @default.
• W2044858025 hasAuthorship W2044858025A5077008742 @default.
• W2044858025 hasBestOaLocation W20448580251 @default.
• W2044858025 hasConcept C104317684 @default.
• W2044858025 hasConcept C107888121 @default.
• W2044858025 hasConcept C120504264 @default.
• W2044858025 hasConcept C141937037 @default.
• W2044858025 hasConcept C1491633281 @default.
• W2044858025 hasConcept C175732170 @default.
• W2044858025 hasConcept C192838845 @default.
• W2044858025 hasConcept C196843134 @default.
• W2044858025 hasConcept C2776690073 @default.
• W2044858025 hasConcept C2780391303 @default.
• W2044858025 hasConcept C2910992960 @default.
• W2044858025 hasConcept C29537977 @default.
• W2044858025 hasConcept C30481170 @default.
• W2044858025 hasConcept C53420372 @default.
• W2044858025 hasConcept C54355233 @default.

• next