FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2044981043> ?p ?o ?g. }

• W2044981043 endingPage "2228" @default.
• W2044981043 startingPage "2218" @default.
• W2044981043 abstract "It was suggested that gap junctional intercellular communication (GJIC) and connexin (Cx) proteins play a crucial role in cell proliferation and differentiation. However, the mechanisms of cell coupling in regulating cell fate during embryonic development are poorly understood. To study the role of GJIC in proliferation and differentiation, we used a human neural progenitor cell line derived from the ventral mesencephalon. Fluorescence recovery after photobleaching (FRAP) showed that dye coupling was extensive in proliferating cells but diminished after the induction of differentiation, as indicated by a 2.5-fold increase of the half-time of fluorescence recovery. Notably, recovery half-time decreased strongly (five-fold) in the later stage of differentiation. Western blot analysis revealed a similar time-dependent expression profile of Cx43, acting as the main gap junction-forming protein. Interestingly, large amounts of cytoplasmic Cx43 were retained mainly in the Golgi network during proliferation but decreased when differentiation was induced. Furthermore, down-regulation of Cx43 by small interfering RNA reduced functional cell coupling, which in turn resulted in a 50% decrease of both the proliferation rate and neuronal differentiation. Our findings suggest a dual function of Cx43 and GJIC in the neural development of ReNcell VM197 human progenitor cells. GJIC accompanied by high Cx43 expression is necessary (1) to maintain cells in a proliferative state and (2) to complete neuronal differentiation, including the establishment of a neural network. However, uncoupling of cells is crucial in the early stage of differentiation during cell fate commitment." @default.
• W2044981043 created "2016-06-24" @default.
• W2044981043 creator A5012794413 @default.
• W2044981043 creator A5026735577 @default.
• W2044981043 creator A5057132188 @default.
• W2044981043 creator A5058157647 @default.
• W2044981043 date "2013-04-22" @default.
• W2044981043 modified "2023-09-27" @default.
• W2044981043 title "Neuronal differentiation requires a biphasic modulation of gap junctional intercellular communication caused by dynamic changes of connexin43 expression" @default.
• W2044981043 cites W1525816166 @default.
• W2044981043 cites W1816808015 @default.
• W2044981043 cites W1904228445 @default.
• W2044981043 cites W1925508137 @default.
• W2044981043 cites W1931134478 @default.
• W2044981043 cites W1965516033 @default.
• W2044981043 cites W1966401658 @default.
• W2044981043 cites W1968311442 @default.
• W2044981043 cites W1968314092 @default.
• W2044981043 cites W1975919509 @default.
• W2044981043 cites W1983460238 @default.
• W2044981043 cites W1987594610 @default.
• W2044981043 cites W1992710821 @default.
• W2044981043 cites W1994866501 @default.
• W2044981043 cites W1998621313 @default.
• W2044981043 cites W2003457113 @default.
• W2044981043 cites W2004543181 @default.
• W2044981043 cites W2008831180 @default.
• W2044981043 cites W2013007296 @default.
• W2044981043 cites W2019946556 @default.
• W2044981043 cites W2024478381 @default.
• W2044981043 cites W2027088984 @default.
• W2044981043 cites W2030743632 @default.
• W2044981043 cites W2036021978 @default.
• W2044981043 cites W2037916969 @default.
• W2044981043 cites W2039824179 @default.
• W2044981043 cites W2047827091 @default.
• W2044981043 cites W2049028460 @default.
• W2044981043 cites W2050844532 @default.
• W2044981043 cites W2068434398 @default.
• W2044981043 cites W2069984314 @default.
• W2044981043 cites W2077474152 @default.
• W2044981043 cites W2080457127 @default.
• W2044981043 cites W2080936939 @default.
• W2044981043 cites W2084065789 @default.
• W2044981043 cites W2084652820 @default.
• W2044981043 cites W2088327864 @default.
• W2044981043 cites W2089976500 @default.
• W2044981043 cites W2091014895 @default.
• W2044981043 cites W2096105873 @default.
• W2044981043 cites W2100971443 @default.
• W2044981043 cites W2110049914 @default.
• W2044981043 cites W2114028154 @default.
• W2044981043 cites W2118450542 @default.
• W2044981043 cites W2119624274 @default.
• W2044981043 cites W2122222586 @default.
• W2044981043 cites W2128145709 @default.
• W2044981043 cites W2134178640 @default.
• W2044981043 cites W2134823614 @default.
• W2044981043 cites W2163056759 @default.
• W2044981043 cites W2165112079 @default.
• W2044981043 cites W2166809896 @default.
• W2044981043 cites W2170802563 @default.
• W2044981043 cites W2323243689 @default.
• W2044981043 cites W2408065810 @default.
• W2044981043 doi "https://doi.org/10.1111/ejn.12219" @default.
• W2044981043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23607708" @default.
• W2044981043 hasPublicationYear "2013" @default.
• W2044981043 type Work @default.
• W2044981043 sameAs 2044981043 @default.
• W2044981043 citedByCount "16" @default.
• W2044981043 countsByYear W20449810432014 @default.
• W2044981043 countsByYear W20449810432015 @default.
• W2044981043 countsByYear W20449810432016 @default.
• W2044981043 countsByYear W20449810432017 @default.
• W2044981043 countsByYear W20449810432018 @default.
• W2044981043 countsByYear W20449810432019 @default.
• W2044981043 countsByYear W20449810432020 @default.
• W2044981043 crossrefType "journal-article" @default.
• W2044981043 hasAuthorship W2044981043A5012794413 @default.
• W2044981043 hasAuthorship W2044981043A5026735577 @default.
• W2044981043 hasAuthorship W2044981043A5057132188 @default.
• W2044981043 hasAuthorship W2044981043A5058157647 @default.
• W2044981043 hasConcept C104317684 @default.
• W2044981043 hasConcept C136834591 @default.
• W2044981043 hasConcept C145103041 @default.
• W2044981043 hasConcept C148738053 @default.
• W2044981043 hasConcept C158157758 @default.
• W2044981043 hasConcept C163952510 @default.
• W2044981043 hasConcept C201750760 @default.
• W2044981043 hasConcept C26659971 @default.
• W2044981043 hasConcept C2780228694 @default.
• W2044981043 hasConcept C28328180 @default.
• W2044981043 hasConcept C41625074 @default.
• W2044981043 hasConcept C4746552 @default.
• W2044981043 hasConcept C54355233 @default.
• W2044981043 hasConcept C62112901 @default.
• W2044981043 hasConcept C79879829 @default.
• W2044981043 hasConcept C86339819 @default.

• next