Matches in SemOpenAlex for { <https://semopenalex.org/work/W2049857900> ?p ?o ?g. }
- W2049857900 endingPage "37" @default.
- W2049857900 startingPage "29" @default.
- W2049857900 abstract "A hydroxyapatite (Hap) monolithic column with micrometer macropores skeleton structure was prepared by sol–gel technique for efficient DNA extraction. The main extraction mechanism of this monolithic column was attributed to the electrostatic interaction between the phosphate groups of DNA and the calcium ions (C site) of Hap. DNA extraction conditions, such as pH, ion concentration, ion type and loading capacity, on the monolithic column were optimized online by capillary electrophoresis with laser-induced fluorescence detection. Under the optimal condition, a 6 cm length monolithic column provided a capacity of 40 ng DNA with an extraction efficiency of 64 ± 6.2% (X ± RSD). As low concentration of salts were used in the extraction procedure, the purified PBE2 plasmid from the Bacillus subtilis crude lysate could be amplified by polymerase chain reaction. This result illustrated that Hap was a potential matrix for DNA purification from complex biological samples which was compatible with the subsequent genetic analysis in miniature format. Since the preparation of this monolithic column was very simple, it was possible to integrate this novel matrix with chip to allow rapid and efficient DNA purification in microscale. This study provided a new attractive solid-phase support for DNA extraction to meet the miniaturized and automated trends of genetic analysis." @default.
- W2049857900 created "2016-06-24" @default.
- W2049857900 creator A5024224542 @default.
- W2049857900 creator A5059795059 @default.
- W2049857900 creator A5076255361 @default.
- W2049857900 creator A5078410835 @default.
- W2049857900 creator A5079951584 @default.
- W2049857900 creator A5087906507 @default.
- W2049857900 date "2008-03-01" @default.
- W2049857900 modified "2023-10-15" @default.
- W2049857900 title "New hydroxyapatite monolithic column for DNA extraction and its application in the purification of Bacillus subtilis crude lysate" @default.
- W2049857900 cites W129129599 @default.
- W2049857900 cites W1531846676 @default.
- W2049857900 cites W1557977383 @default.
- W2049857900 cites W1606713702 @default.
- W2049857900 cites W1975759686 @default.
- W2049857900 cites W1977795843 @default.
- W2049857900 cites W1979531777 @default.
- W2049857900 cites W1980349723 @default.
- W2049857900 cites W1980761392 @default.
- W2049857900 cites W1984416177 @default.
- W2049857900 cites W1988254877 @default.
- W2049857900 cites W1991820771 @default.
- W2049857900 cites W1996403408 @default.
- W2049857900 cites W1997887128 @default.
- W2049857900 cites W2013320349 @default.
- W2049857900 cites W2017060302 @default.
- W2049857900 cites W2024512976 @default.
- W2049857900 cites W2024960184 @default.
- W2049857900 cites W2025960727 @default.
- W2049857900 cites W2028630994 @default.
- W2049857900 cites W2029289940 @default.
- W2049857900 cites W2031411216 @default.
- W2049857900 cites W2038494950 @default.
- W2049857900 cites W2039483927 @default.
- W2049857900 cites W2041741920 @default.
- W2049857900 cites W2042893922 @default.
- W2049857900 cites W2044379378 @default.
- W2049857900 cites W2050619834 @default.
- W2049857900 cites W2065817433 @default.
- W2049857900 cites W2067049506 @default.
- W2049857900 cites W2068089964 @default.
- W2049857900 cites W2073221683 @default.
- W2049857900 cites W2073598279 @default.
- W2049857900 cites W2082105107 @default.
- W2049857900 cites W2086335675 @default.
- W2049857900 cites W2094010576 @default.
- W2049857900 cites W2147015873 @default.
- W2049857900 cites W2150295022 @default.
- W2049857900 cites W2150704355 @default.
- W2049857900 cites W4250116836 @default.
- W2049857900 doi "https://doi.org/10.1016/j.chroma.2007.11.120" @default.
- W2049857900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18255077" @default.
- W2049857900 hasPublicationYear "2008" @default.
- W2049857900 type Work @default.
- W2049857900 sameAs 2049857900 @default.
- W2049857900 citedByCount "37" @default.
- W2049857900 countsByYear W20498579002012 @default.
- W2049857900 countsByYear W20498579002013 @default.
- W2049857900 countsByYear W20498579002015 @default.
- W2049857900 countsByYear W20498579002016 @default.
- W2049857900 countsByYear W20498579002017 @default.
- W2049857900 countsByYear W20498579002018 @default.
- W2049857900 countsByYear W20498579002019 @default.
- W2049857900 countsByYear W20498579002020 @default.
- W2049857900 countsByYear W20498579002021 @default.
- W2049857900 crossrefType "journal-article" @default.
- W2049857900 hasAuthorship W2049857900A5024224542 @default.
- W2049857900 hasAuthorship W2049857900A5059795059 @default.
- W2049857900 hasAuthorship W2049857900A5076255361 @default.
- W2049857900 hasAuthorship W2049857900A5078410835 @default.
- W2049857900 hasAuthorship W2049857900A5079951584 @default.
- W2049857900 hasAuthorship W2049857900A5087906507 @default.
- W2049857900 hasConcept C104317684 @default.
- W2049857900 hasConcept C106487976 @default.
- W2049857900 hasConcept C134871310 @default.
- W2049857900 hasConcept C145420912 @default.
- W2049857900 hasConcept C179428855 @default.
- W2049857900 hasConcept C179998833 @default.
- W2049857900 hasConcept C185592680 @default.
- W2049857900 hasConcept C2777272437 @default.
- W2049857900 hasConcept C33923547 @default.
- W2049857900 hasConcept C43617362 @default.
- W2049857900 hasConcept C4725764 @default.
- W2049857900 hasConcept C49105822 @default.
- W2049857900 hasConcept C51724486 @default.
- W2049857900 hasConcept C523546767 @default.
- W2049857900 hasConcept C54355233 @default.
- W2049857900 hasConcept C552990157 @default.
- W2049857900 hasConcept C55493867 @default.
- W2049857900 hasConcept C57409179 @default.
- W2049857900 hasConcept C78063203 @default.
- W2049857900 hasConcept C81426234 @default.
- W2049857900 hasConcept C86803240 @default.
- W2049857900 hasConceptScore W2049857900C104317684 @default.
- W2049857900 hasConceptScore W2049857900C106487976 @default.
- W2049857900 hasConceptScore W2049857900C134871310 @default.
- W2049857900 hasConceptScore W2049857900C145420912 @default.