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- W2054882215 abstract "The leafhopper Euscelis incisus contains two morphologically-distinct symbiotic microorganisms, a-symbionts and t-symbionts, in a pair of discrete organs, the mycetomes. Pure preparations of t-symbionts were obtained by lysis of insect cells with 0.05% digitonin, followed by density-gradient centrifugation, but the a-symbionts could not be isolated. Symbiont-free E. incisus were obtained by ligation of newly deposited eggs. The embryos developed normally to hatching, but the larvae did not grow and died in 2 weeks. The mycetome symbionts were not disrupted in larvae feeding on plants with the antibiotic chlortetracycline at 60 μg g−1 shoot but the guts of these insects lacked microorganisms and the larvae grew slowly and died in the second or third instar. Control insects had a substantial gut microflora. It is concluded that both the mycetome symbionts and the gut flora are required for normal growth and development of larval E. incisus. The incorporation of [14C]leucine into protein by isolated mycetomes of E. incisus was insensitive to chloramphenicol, an inhibitor of prokaryotic protein synthesis. It is suggested that the mycetome symbionts synthesise protein at very low rates and this may account for their insensitivity to chlortetracycline." @default.
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- W2054882215 date "1988-01-01" @default.
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- W2054882215 title "Experimental studies on the mycetome symbiosis in the leafhopper Euscelis incisus" @default.
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- W2054882215 doi "https://doi.org/10.1016/0022-1910(88)90204-1" @default.
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