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• W2068362526 abstract "In this paper, we report the use of micellar electrokinetic chromatography (MEKC) for the highly efficient preconcentration and separation of gold nanoparticles (Au NPs). We used the reversed electrode polarity stacking mode (REPSM) of the MEKC system for the on-line enhancement and separation of the Au NPs. Several parameters had dramatic effects on the systems’ performance, including the concentration of sodium dodecylsulfate (SDS) surfactant, the presence of salts in the NP solution, the pH of the running electrolyte, and the temperature of the capillary. Under the optimized conditions [buffer: SDS (70 mM) and 3-cyclohexylamino-1-propanesulfonic acid (CAPS; 10 mM) at pH 10.0; applied voltage: 20 kV; operating temperature: 25 °C; additive: sodium dihydrogenphosphate (NaH2PO4, 10 mM); REPSM strategy for sample preconcentration], the number of theoretical plates for the 5.3- and 40.1-nm-diameter Au NPs were 3000 and (an ultrahigh) 2.1 × 106, respectively; in addition, the detection sensitivities toward the Au NPs were enhanced ca. 20- and 380-fold, respectively, relative to those obtained using standard MEKC analysis conditions. Furthermore, monitoring the electropherograms using diode-array detection allowed us to identify and characterize the sizes of the separated NPs from their UV–vis spectra. Our findings suggest that MEKC is a highly efficient tool for both the preconcentration and separation of NPs." @default.
• W2068362526 created "2016-06-24" @default.
• W2068362526 creator A5090338507 @default.
• W2068362526 date "2009-03-01" @default.
• W2068362526 modified "2023-09-23" @default.
• W2068362526 title "Using micellar electrokinetic chromatography for the highly efficient preconcentration and separation of gold nanoparticles" @default.
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• W2068362526 doi "https://doi.org/10.1016/j.chroma.2009.01.004" @default.
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