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• W2068476020 abstract "Three different mini-Tn5 plasmids, containing a tetracycline-inducible promoter, Ptet and a regulatory gene, tetR, in operon fusions with a reporter gene system (lacZYA, luxCDABE or gfp), were constructed. These biosensor constructs responded to low levels of tetracyclines by producing β-galactosidase, light or green fluorescent protein. They did so in a quantitative manner, thus enabling the quantification of tetracyclines in the immediate surroundings of the biosensor organism. All three constructs were transferred successfully to different Gram-negative bacteria by conjugation. An Escherichia coli strain containing the Ptet-lac construct was used to determine oxytetracycline in milk as a demonstration of the application of these biosensors." @default.
• W2068476020 created "2016-06-24" @default.
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• W2068476020 creator A5090059968 @default.
• W2068476020 date "2000-09-01" @default.
• W2068476020 modified "2023-09-27" @default.
• W2068476020 title "Detection and quantification of tetracyclines by whole cell biosensors" @default.
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• W2068476020 doi "https://doi.org/10.1111/j.1574-6968.2000.tb09298.x" @default.
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