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- W2069588595 abstract "Abstract Feulgen-DNA hydrolysis time curves were constructed for nuclei isolated from meristematic and differentiated parenchymal cells of the cultured orchid embryo using a microfluorometric technique. These nuclei correspond to “diploid” and “polyploid” DNA classes. The results verify our previous observations based on absorption measurements that Feulgen dye binding increases with nuclear size and in discrete increments with differentiation of the parenchyma up to values of 8C. The hydrolysis time curves from meristematic and normal parenchymal cells were of identical form, both showing two peaks at 30 and 70 min. The data indicate that hydrolysis time does not affect the proportionality between the amount of dye bound and nuclear size. The hydrolysis time curve from senescent parenchymal cells, on the other hand, differs from those of the meristematic and normal differentiated cells in that a sharp first peak occurs at 5 min with a second at 30 min. The effect of several methodological parameters on the application of microspectrofluorometry for Feulgen determinations is discussed." @default.
- W2069588595 created "2016-06-24" @default.
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- W2069588595 date "1970-07-01" @default.
- W2069588595 modified "2023-09-25" @default.
- W2069588595 title "Microfluorometric comparison of Feulgen-deoxyribonucleic acid hydrolysis in meristematic and differentiated cells of the orchid embryo" @default.
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- W2069588595 doi "https://doi.org/10.1016/0014-4827(70)90273-9" @default.
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