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- W2071044364 abstract "The in vitro fertilizing competence of fresh and frozen epididymal sperm of domestic cat was compared. The average portions of intact and motile spermatozoa amounted to 42.8% and 75%, respectively, immediately after preparation, and decreased after cryopreservation to values of 27.8% and 53%, respectively. Enrichment of vital frozen spermatozoa by a swim-up procedure provided a motility level similar to that of fresh spermatozoa (74%). The spermatozoa showed a high rate of spontaneous acrosome loss, especially after thawing. Therefore, the number of intact frozen cells with induced acrosome reaction (0.005% hyamine for 20 min at 38°C) was reduced significantly. Oocytes were obtained from antral follicles of ovariectomized cats, matured in vitro for 48 h and inseminated in vitro with fresh or frozen spermatozoa. The insemination doses contained comparable sperm concentrations and percentages of motile spermatozoa. However, the cleavage rates were significantly lower for zygotes produced by frozen spermatozoa (43170 = 25.3%) than for zygotes produced by fresh spermatozoa (61150=40.7%). The rates of production of cat embryos using epididymal sperm might be a useful basis for application of these techniques in other feline species." @default.
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- W2071044364 date "1994-05-01" @default.
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- W2071044364 title "In vitro fertilization of follicular oocytes of domestic cat using fresh and cryopreserved epididymal spermatozoa" @default.
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- W2071044364 doi "https://doi.org/10.1016/0378-4320(94)90044-2" @default.
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