FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071702989> ?p ?o ?g. }

• W2071702989 endingPage "548" @default.
• W2071702989 startingPage "543" @default.
• W2071702989 abstract "Luciferase assay has become an increasingly important technique to monitor a wide range of biological processes. However, the mainstay protocols require a luminometer to acquire and process the data, therefore limiting its application to specialized research labs. To overcome this limitation, we have developed an alternative protocol that utilizes a commonly available cooled charge-coupled device (CCCD), instead of a luminometer for data acquiring and processing. By measuring activities of different luciferases, we characterized their substrate specificity, assay linearity, signal-to-noise levels, and fold-changes via CCCD. Next, we defined the assay parameters that are critical for appropriate use of CCCD for different luciferases. To demonstrate the usefulness in cultured mammalian cells, we conducted a case study to examine NFκB gene activation in response to inflammatory signals in human embryonic kidney cells (HEK293 cells). We found that data collected by CCCD camera was equivalent to those acquired by luminometer, thus validating the assay protocol. In comparison, The CCCD-based protocol is readily amenable to live-cell and high-throughput applications, offering fast simultaneous data acquisition and visual and quantitative data presentation. In conclusion, the CCCD-based protocol provides a useful alternative for monitoring luciferase reporters. The wide availability of CCCD will enable more researchers to use luciferases to monitor and quantify biological processes." @default.
• W2071702989 created "2016-06-24" @default.
• W2071702989 creator A5011986684 @default.
• W2071702989 creator A5031085364 @default.
• W2071702989 creator A5051683946 @default.
• W2071702989 date "2015-03-01" @default.
• W2071702989 modified "2023-10-02" @default.
• W2071702989 title "A cooled CCD camera-based protocol provides an effective solution for in vitro monitoring of luciferase" @default.
• W2071702989 cites W1591311438 @default.
• W2071702989 cites W1964879325 @default.
• W2071702989 cites W1968603674 @default.
• W2071702989 cites W1980675215 @default.
• W2071702989 cites W1993378838 @default.
• W2071702989 cites W1994357942 @default.
• W2071702989 cites W2007867011 @default.
• W2071702989 cites W2010747482 @default.
• W2071702989 cites W2028731012 @default.
• W2071702989 cites W2052301777 @default.
• W2071702989 cites W2054480995 @default.
• W2071702989 cites W2063119860 @default.
• W2071702989 cites W2068408712 @default.
• W2071702989 cites W2068783587 @default.
• W2071702989 cites W2080314388 @default.
• W2071702989 cites W2083797848 @default.
• W2071702989 cites W2092871526 @default.
• W2071702989 cites W2094709822 @default.
• W2071702989 cites W2097794242 @default.
• W2071702989 cites W2099994710 @default.
• W2071702989 cites W2103049653 @default.
• W2071702989 cites W2104200873 @default.
• W2071702989 cites W2105177503 @default.
• W2071702989 cites W2106685495 @default.
• W2071702989 cites W2118519588 @default.
• W2071702989 cites W2126715596 @default.
• W2071702989 cites W2131003736 @default.
• W2071702989 cites W2140478568 @default.
• W2071702989 cites W2158227899 @default.
• W2071702989 cites W2160570153 @default.
• W2071702989 cites W2170405846 @default.
• W2071702989 cites W2170997336 @default.
• W2071702989 cites W4237304395 @default.
• W2071702989 doi "https://doi.org/10.1016/j.bbrc.2015.01.150" @default.
• W2071702989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25677617" @default.
• W2071702989 hasPublicationYear "2015" @default.
• W2071702989 type Work @default.
• W2071702989 sameAs 2071702989 @default.
• W2071702989 citedByCount "9" @default.
• W2071702989 countsByYear W20717029892015 @default.
• W2071702989 countsByYear W20717029892017 @default.
• W2071702989 countsByYear W20717029892019 @default.
• W2071702989 countsByYear W20717029892023 @default.
• W2071702989 crossrefType "journal-article" @default.
• W2071702989 hasAuthorship W2071702989A5011986684 @default.
• W2071702989 hasAuthorship W2071702989A5031085364 @default.
• W2071702989 hasAuthorship W2071702989A5051683946 @default.
• W2071702989 hasConcept C111335760 @default.
• W2071702989 hasConcept C142724271 @default.
• W2071702989 hasConcept C204787440 @default.
• W2071702989 hasConcept C2780385302 @default.
• W2071702989 hasConcept C2909536089 @default.
• W2071702989 hasConcept C41008148 @default.
• W2071702989 hasConcept C54009773 @default.
• W2071702989 hasConcept C54355233 @default.
• W2071702989 hasConcept C70721500 @default.
• W2071702989 hasConcept C71924100 @default.
• W2071702989 hasConcept C81885089 @default.
• W2071702989 hasConcept C86803240 @default.
• W2071702989 hasConceptScore W2071702989C111335760 @default.
• W2071702989 hasConceptScore W2071702989C142724271 @default.
• W2071702989 hasConceptScore W2071702989C204787440 @default.
• W2071702989 hasConceptScore W2071702989C2780385302 @default.
• W2071702989 hasConceptScore W2071702989C2909536089 @default.
• W2071702989 hasConceptScore W2071702989C41008148 @default.
• W2071702989 hasConceptScore W2071702989C54009773 @default.
• W2071702989 hasConceptScore W2071702989C54355233 @default.
• W2071702989 hasConceptScore W2071702989C70721500 @default.
• W2071702989 hasConceptScore W2071702989C71924100 @default.
• W2071702989 hasConceptScore W2071702989C81885089 @default.
• W2071702989 hasConceptScore W2071702989C86803240 @default.
• W2071702989 hasIssue "3" @default.
• W2071702989 hasLocation W20717029891 @default.
• W2071702989 hasLocation W20717029892 @default.
• W2071702989 hasOpenAccess W2071702989 @default.
• W2071702989 hasPrimaryLocation W20717029891 @default.
• W2071702989 hasRelatedWork W1937835538 @default.
• W2071702989 hasRelatedWork W2065710197 @default.
• W2071702989 hasRelatedWork W2104093223 @default.
• W2071702989 hasRelatedWork W2119771998 @default.
• W2071702989 hasRelatedWork W2151818001 @default.
• W2071702989 hasRelatedWork W2589642557 @default.
• W2071702989 hasRelatedWork W2792099120 @default.
• W2071702989 hasRelatedWork W4236965099 @default.
• W2071702989 hasRelatedWork W4360602712 @default.
• W2071702989 hasRelatedWork W53587174 @default.
• W2071702989 hasVolume "458" @default.
• W2071702989 isParatext "false" @default.
• W2071702989 isRetracted "false" @default.
• W2071702989 magId "2071702989" @default.

• next