Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077936098> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2077936098 endingPage "1700" @default.
- W2077936098 startingPage "1695" @default.
- W2077936098 abstract "The properties of the recently characterized vanadium-dependent nitrogenase system of Azotobacter are reviewed and compared with the better characterized Mo-nitrogenases. Vanadium is present in V-nitrogenase in a vanadium- and iron-containing protein (VFe protein), analogous in structure and function to the MoFe protein of Mo-nitrogenase. The VFe protein contains vanadium in a polynuclear cluster in an environment with iron, sulphur and oxygen (or nitrogen or carbon) as nearest neighbour atoms, very similar to the environment of molybdenum in the iron and molybdenum cofactor (FeMoco) centre of the MoFe proteins. A vanadium-containing cluster can be extracted from the VFe protein by treatment with N-methylformamide (NMF). The NMF extract contains vanadium, iron and sulphur in a 1:6:5 ratio and activates the inactive MoFe protein synthesized by some mutant organisms unable to synthesize FeMoco. This hybrid protein has the characteristic substrate reduction pattern of a VFe protein, in forming some C2H6 from C2H2. These data are consistent with the NMF extract containing an iron and vanadium cofactor FeVaco, analogous to FeMoco. Although the hybrid protein is active in nitrogenase assays with H+ or C2H2 as reducible substrates, N2 is not reduced, suggesting that specific interactions of the cofactor centre with a polypeptide ligand are lacking. Pre-steady-state kinetics of the ATP-dependent electron-transfer from the Fe protein of V-nitrogenase to a VFe protein are very similar to those of Mo-nitrogenase components, consistent with a common mechanism of substrate reduction. However, at low temperatures N2 is a more effective substrate for V-nitrogenase compared with Mo-nitrogenase." @default.
- W2077936098 created "2016-06-24" @default.
- W2077936098 creator A5032668767 @default.
- W2077936098 date "1989-01-01" @default.
- W2077936098 modified "2023-09-23" @default.
- W2077936098 title "The vanadium nitrogenase of Azotobacter" @default.
- W2077936098 cites W1495034155 @default.
- W2077936098 cites W1558640256 @default.
- W2077936098 cites W1565442899 @default.
- W2077936098 cites W1587863598 @default.
- W2077936098 cites W1592733141 @default.
- W2077936098 cites W1611316793 @default.
- W2077936098 cites W1849451321 @default.
- W2077936098 cites W1873954035 @default.
- W2077936098 cites W1877000601 @default.
- W2077936098 cites W1942904925 @default.
- W2077936098 cites W1977742856 @default.
- W2077936098 cites W198948238 @default.
- W2077936098 cites W2002435060 @default.
- W2077936098 cites W2012293227 @default.
- W2077936098 cites W2024455262 @default.
- W2077936098 cites W2025124026 @default.
- W2077936098 cites W2032590074 @default.
- W2077936098 cites W2062298430 @default.
- W2077936098 cites W2073070845 @default.
- W2077936098 cites W2079240101 @default.
- W2077936098 cites W2085218045 @default.
- W2077936098 cites W2087438947 @default.
- W2077936098 cites W2123018127 @default.
- W2077936098 cites W2176227180 @default.
- W2077936098 cites W2270437609 @default.
- W2077936098 cites W2338177616 @default.
- W2077936098 cites W4213031625 @default.
- W2077936098 cites W7864952 @default.
- W2077936098 doi "https://doi.org/10.1016/s0277-5387(00)80619-1" @default.
- W2077936098 hasPublicationYear "1989" @default.
- W2077936098 type Work @default.
- W2077936098 sameAs 2077936098 @default.
- W2077936098 citedByCount "23" @default.
- W2077936098 countsByYear W20779360982014 @default.
- W2077936098 countsByYear W20779360982019 @default.
- W2077936098 countsByYear W20779360982021 @default.
- W2077936098 crossrefType "journal-article" @default.
- W2077936098 hasAuthorship W2077936098A5032668767 @default.
- W2077936098 hasConcept C134621786 @default.
- W2077936098 hasConcept C141280058 @default.
- W2077936098 hasConcept C178790620 @default.
- W2077936098 hasConcept C179104552 @default.
- W2077936098 hasConcept C181199279 @default.
- W2077936098 hasConcept C181440489 @default.
- W2077936098 hasConcept C185592680 @default.
- W2077936098 hasConcept C197957613 @default.
- W2077936098 hasConcept C2776159390 @default.
- W2077936098 hasConcept C504678139 @default.
- W2077936098 hasConcept C537208039 @default.
- W2077936098 hasConcept C549387045 @default.
- W2077936098 hasConcept C55493867 @default.
- W2077936098 hasConcept C71240020 @default.
- W2077936098 hasConcept C8010536 @default.
- W2077936098 hasConceptScore W2077936098C134621786 @default.
- W2077936098 hasConceptScore W2077936098C141280058 @default.
- W2077936098 hasConceptScore W2077936098C178790620 @default.
- W2077936098 hasConceptScore W2077936098C179104552 @default.
- W2077936098 hasConceptScore W2077936098C181199279 @default.
- W2077936098 hasConceptScore W2077936098C181440489 @default.
- W2077936098 hasConceptScore W2077936098C185592680 @default.
- W2077936098 hasConceptScore W2077936098C197957613 @default.
- W2077936098 hasConceptScore W2077936098C2776159390 @default.
- W2077936098 hasConceptScore W2077936098C504678139 @default.
- W2077936098 hasConceptScore W2077936098C537208039 @default.
- W2077936098 hasConceptScore W2077936098C549387045 @default.
- W2077936098 hasConceptScore W2077936098C55493867 @default.
- W2077936098 hasConceptScore W2077936098C71240020 @default.
- W2077936098 hasConceptScore W2077936098C8010536 @default.
- W2077936098 hasIssue "13-14" @default.
- W2077936098 hasLocation W20779360981 @default.
- W2077936098 hasOpenAccess W2077936098 @default.
- W2077936098 hasPrimaryLocation W20779360981 @default.
- W2077936098 hasRelatedWork W1969816885 @default.
- W2077936098 hasRelatedWork W1983613885 @default.
- W2077936098 hasRelatedWork W2092835915 @default.
- W2077936098 hasRelatedWork W2490664556 @default.
- W2077936098 hasRelatedWork W2560222515 @default.
- W2077936098 hasRelatedWork W2989961524 @default.
- W2077936098 hasRelatedWork W3084915122 @default.
- W2077936098 hasRelatedWork W3186257491 @default.
- W2077936098 hasRelatedWork W617180500 @default.
- W2077936098 hasRelatedWork W618994457 @default.
- W2077936098 hasVolume "8" @default.
- W2077936098 isParatext "false" @default.
- W2077936098 isRetracted "false" @default.
- W2077936098 magId "2077936098" @default.
- W2077936098 workType "article" @default.