Matches in SemOpenAlex for { <https://semopenalex.org/work/W2078135973> ?p ?o ?g. }
- W2078135973 abstract "The aim of the study was to analyze the gene expression profile of pancreatic cancer to derive novel molecular markers of this malignancy. The snap-frozen or RNA-later preserved samples of 18 pancreatic adenocarcinomas, 5 chronic pancreatitis cases and 6 specimens of grossly normal pancreas were used for microarray analysis by HG-U133 Plus 2.0 oligonucleotide Affymetrix arrays. Validation was carried out by real-time quantitative PCR (Q-PCR) in the set of 66 samples: 31 of pancreatic cancer, 14 of chronic pancreatitis and 21 of macroscopically unchanged pancreas. By Principal Component Analysis of the microarray data we found a very consistent expression pattern of normal samples and a less homogenous one in chronic pancreatitis. By supervised comparison (corrected p-value 0.001) we observed 11094 probesets differentiating between cancer and normal samples, while only seventy six probesets were significant for difference between cancer and chronic pancreatitis. The only gene occurring within the best 10 genes in both comparisons was S100 calcium binding protein P (S100P), already indicated for its utility as pancreatic cancer marker by earlier microarray-based studies. For validation we selected two genes which appeared as valuable candidates for molecular markers of pancreatic cancer: neuroblastoma, suppression of tumorigenicity 1 (NBL1) and anillin (ANLN). By Q-PCR, we confirmed statistically significant differences in these genes with a 9.5 fold-change difference between NBL1 expression in cancer/normal comparison and a relatively modest difference between cancer and pancreatitis. For ANLN even more distinct differences were observed (cancer/normal 19.8-fold, cancer/pancreatitis 4.0-fold). NBL1 and anillin are promising markers for pancreatic carcinoma molecular diagnostics." @default.
- W2078135973 created "2016-06-24" @default.
- W2078135973 creator A5001501320 @default.
- W2078135973 creator A5003851108 @default.
- W2078135973 creator A5008391401 @default.
- W2078135973 creator A5009908756 @default.
- W2078135973 creator A5016379728 @default.
- W2078135973 creator A5017076329 @default.
- W2078135973 creator A5023706702 @default.
- W2078135973 creator A5024679986 @default.
- W2078135973 creator A5031383867 @default.
- W2078135973 creator A5037159891 @default.
- W2078135973 creator A5041097532 @default.
- W2078135973 creator A5057790335 @default.
- W2078135973 creator A5058514961 @default.
- W2078135973 creator A5063905359 @default.
- W2078135973 date "2009-12-10" @default.
- W2078135973 modified "2023-10-01" @default.
- W2078135973 title "NBL1 and anillin (ANLN) genes over-expression in pancreatic carcinoma." @default.
- W2078135973 cites W1577577364 @default.
- W2078135973 cites W1599187838 @default.
- W2078135973 cites W1978265829 @default.
- W2078135973 cites W1979655901 @default.
- W2078135973 cites W1982698039 @default.
- W2078135973 cites W1988973275 @default.
- W2078135973 cites W2009398566 @default.
- W2078135973 cites W2010168796 @default.
- W2078135973 cites W2011550114 @default.
- W2078135973 cites W2022739920 @default.
- W2078135973 cites W2027210742 @default.
- W2078135973 cites W2029023103 @default.
- W2078135973 cites W2049863303 @default.
- W2078135973 cites W2056685208 @default.
- W2078135973 cites W2058230583 @default.
- W2078135973 cites W2082498204 @default.
- W2078135973 cites W2088710192 @default.
- W2078135973 cites W2090014102 @default.
- W2078135973 cites W2093262421 @default.
- W2078135973 cites W2101163066 @default.
- W2078135973 cites W2103543394 @default.
- W2078135973 cites W2104327265 @default.
- W2078135973 cites W2107632463 @default.
- W2078135973 cites W2109986840 @default.
- W2078135973 cites W2121533951 @default.
- W2078135973 cites W2124498637 @default.
- W2078135973 cites W2126031968 @default.
- W2078135973 cites W2133408651 @default.
- W2078135973 cites W2149753171 @default.
- W2078135973 doi "https://doi.org/10.2478/v10042-009-0031-1" @default.
- W2078135973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19995712" @default.
- W2078135973 hasPublicationYear "2009" @default.
- W2078135973 type Work @default.
- W2078135973 sameAs 2078135973 @default.
- W2078135973 citedByCount "48" @default.
- W2078135973 countsByYear W20781359732012 @default.
- W2078135973 countsByYear W20781359732013 @default.
- W2078135973 countsByYear W20781359732014 @default.
- W2078135973 countsByYear W20781359732015 @default.
- W2078135973 countsByYear W20781359732016 @default.
- W2078135973 countsByYear W20781359732017 @default.
- W2078135973 countsByYear W20781359732018 @default.
- W2078135973 countsByYear W20781359732019 @default.
- W2078135973 countsByYear W20781359732020 @default.
- W2078135973 countsByYear W20781359732021 @default.
- W2078135973 countsByYear W20781359732022 @default.
- W2078135973 countsByYear W20781359732023 @default.
- W2078135973 crossrefType "journal-article" @default.
- W2078135973 hasAuthorship W2078135973A5001501320 @default.
- W2078135973 hasAuthorship W2078135973A5003851108 @default.
- W2078135973 hasAuthorship W2078135973A5008391401 @default.
- W2078135973 hasAuthorship W2078135973A5009908756 @default.
- W2078135973 hasAuthorship W2078135973A5016379728 @default.
- W2078135973 hasAuthorship W2078135973A5017076329 @default.
- W2078135973 hasAuthorship W2078135973A5023706702 @default.
- W2078135973 hasAuthorship W2078135973A5024679986 @default.
- W2078135973 hasAuthorship W2078135973A5031383867 @default.
- W2078135973 hasAuthorship W2078135973A5037159891 @default.
- W2078135973 hasAuthorship W2078135973A5041097532 @default.
- W2078135973 hasAuthorship W2078135973A5057790335 @default.
- W2078135973 hasAuthorship W2078135973A5058514961 @default.
- W2078135973 hasAuthorship W2078135973A5063905359 @default.
- W2078135973 hasBestOaLocation W20781359731 @default.
- W2078135973 hasConcept C104317684 @default.
- W2078135973 hasConcept C121608353 @default.
- W2078135973 hasConcept C126322002 @default.
- W2078135973 hasConcept C134018914 @default.
- W2078135973 hasConcept C144369459 @default.
- W2078135973 hasConcept C150194340 @default.
- W2078135973 hasConcept C153911025 @default.
- W2078135973 hasConcept C18431079 @default.
- W2078135973 hasConcept C186836561 @default.
- W2078135973 hasConcept C2775967933 @default.
- W2078135973 hasConcept C2778764654 @default.
- W2078135973 hasConcept C2779399171 @default.
- W2078135973 hasConcept C2780210213 @default.
- W2078135973 hasConcept C2781397275 @default.
- W2078135973 hasConcept C502942594 @default.
- W2078135973 hasConcept C54355233 @default.
- W2078135973 hasConcept C71924100 @default.
- W2078135973 hasConcept C8415881 @default.