FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083000982> ?p ?o ?g. }

• W2083000982 endingPage "2181" @default.
• W2083000982 startingPage "2174" @default.
• W2083000982 abstract "Differences in salt bridges are believed to be a structural hallmark of homologous enzymes from differently temperature-adapted organisms. Nevertheless, the role of salt bridges on structural stability is still controversial. While it is clear that most buried salt bridges can have a functional or structural role, the same cannot be firmly stated for ion pairs that are exposed on the protein surface. Salt bridges, found in X-ray structures, may not be stably formed in solution as a result of high flexibility or high desolvation penalty. More studies are thus needed to clarify the picture on salt bridges and temperature adaptation. We contribute here to this scenario by combining atomistic simulations and experimental mutagenesis of eight mutant variants of aqualysin I, a thermophilic subtilisin-like proteinase, in which the residues involved in salt bridges and not conserved in a psychrophilic homolog were systematically mutated. We evaluated the effects of those mutations on thermal stability and on the kinetic parameters. Overall, we show here that only few key charged residues involved in salt bridges really contribute to the enzyme thermal stability. This is especially true when they are organized in networks, as here attested by the D17N mutation, which has the most remarkable effect on stability. Other mutations had smaller effects on the properties of the enzyme indicating that most of the isolated salt bridges are not a distinctive trait related to the enhanced thermal stability of the thermophilic subtilase." @default.
• W2083000982 created "2016-06-24" @default.
• W2083000982 creator A5021852685 @default.
• W2083000982 creator A5038709593 @default.
• W2083000982 creator A5052201074 @default.
• W2083000982 creator A5061782571 @default.
• W2083000982 creator A5084150613 @default.
• W2083000982 creator A5085464733 @default.
• W2083000982 creator A5089976447 @default.
• W2083000982 date "2014-12-01" @default.
• W2083000982 modified "2023-09-27" @default.
• W2083000982 title "The role of salt bridges on the temperature adaptation of aqualysin I, a thermostable subtilisin-like proteinase" @default.
• W2083000982 cites W1522104226 @default.
• W2083000982 cites W1538879287 @default.
• W2083000982 cites W1608623766 @default.
• W2083000982 cites W1788981747 @default.
• W2083000982 cites W1865157307 @default.
• W2083000982 cites W1965817524 @default.
• W2083000982 cites W1967712722 @default.
• W2083000982 cites W1967762080 @default.
• W2083000982 cites W1968416749 @default.
• W2083000982 cites W1974090741 @default.
• W2083000982 cites W1976981353 @default.
• W2083000982 cites W1981122373 @default.
• W2083000982 cites W1986560888 @default.
• W2083000982 cites W1986638625 @default.
• W2083000982 cites W1987993651 @default.
• W2083000982 cites W1992888813 @default.
• W2083000982 cites W1993977185 @default.
• W2083000982 cites W1995730663 @default.
• W2083000982 cites W1996343552 @default.
• W2083000982 cites W1998902553 @default.
• W2083000982 cites W2003628452 @default.
• W2083000982 cites W2015110061 @default.
• W2083000982 cites W2015536590 @default.
• W2083000982 cites W2023490488 @default.
• W2083000982 cites W2026249442 @default.
• W2083000982 cites W2027145316 @default.
• W2083000982 cites W2033805080 @default.
• W2083000982 cites W2041833185 @default.
• W2083000982 cites W2044690174 @default.
• W2083000982 cites W2048846412 @default.
• W2083000982 cites W2054161985 @default.
• W2083000982 cites W2057477511 @default.
• W2083000982 cites W2059671784 @default.
• W2083000982 cites W2060757799 @default.
• W2083000982 cites W2063868681 @default.
• W2083000982 cites W2064136436 @default.
• W2083000982 cites W2067174909 @default.
• W2083000982 cites W2072612827 @default.
• W2083000982 cites W2075460992 @default.
• W2083000982 cites W2085381964 @default.
• W2083000982 cites W2087293973 @default.
• W2083000982 cites W2087388427 @default.
• W2083000982 cites W2094823743 @default.
• W2083000982 cites W2095118562 @default.
• W2083000982 cites W2096299253 @default.
• W2083000982 cites W2105923547 @default.
• W2083000982 cites W2106320506 @default.
• W2083000982 cites W2121400889 @default.
• W2083000982 cites W2128572087 @default.
• W2083000982 cites W2132589721 @default.
• W2083000982 cites W2136086532 @default.
• W2083000982 cites W2141179031 @default.
• W2083000982 cites W2149395069 @default.
• W2083000982 cites W2151668433 @default.
• W2083000982 cites W2153195964 @default.
• W2083000982 cites W2156470306 @default.
• W2083000982 cites W2158543301 @default.
• W2083000982 cites W2161006313 @default.
• W2083000982 cites W2163408172 @default.
• W2083000982 cites W2168095997 @default.
• W2083000982 cites W2171807422 @default.
• W2083000982 cites W2334874683 @default.
• W2083000982 cites W30218150 @default.
• W2083000982 doi "https://doi.org/10.1016/j.bbapap.2014.08.011" @default.
• W2083000982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25172393" @default.
• W2083000982 hasPublicationYear "2014" @default.
• W2083000982 type Work @default.
• W2083000982 sameAs 2083000982 @default.
• W2083000982 citedByCount "40" @default.
• W2083000982 countsByYear W20830009822015 @default.
• W2083000982 countsByYear W20830009822016 @default.
• W2083000982 countsByYear W20830009822017 @default.
• W2083000982 countsByYear W20830009822018 @default.
• W2083000982 countsByYear W20830009822019 @default.
• W2083000982 countsByYear W20830009822020 @default.
• W2083000982 countsByYear W20830009822021 @default.
• W2083000982 countsByYear W20830009822022 @default.
• W2083000982 countsByYear W20830009822023 @default.
• W2083000982 crossrefType "journal-article" @default.
• W2083000982 hasAuthorship W2083000982A5021852685 @default.
• W2083000982 hasAuthorship W2083000982A5038709593 @default.
• W2083000982 hasAuthorship W2083000982A5052201074 @default.
• W2083000982 hasAuthorship W2083000982A5061782571 @default.
• W2083000982 hasAuthorship W2083000982A5084150613 @default.
• W2083000982 hasAuthorship W2083000982A5085464733 @default.
• W2083000982 hasAuthorship W2083000982A5089976447 @default.

• next