Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097061885> ?p ?o ?g. }
- W2097061885 endingPage "5654" @default.
- W2097061885 startingPage "5646" @default.
- W2097061885 abstract "Directed evolution is a broadly successful strategy for protein engineering in the quest to enhance the stereoselectivity, activity, and thermostability of enzymes. To increase the efficiency of directed evolution based on iterative saturation mutagenesis, the adaptive substituent reordering algorithm (ASRA) is introduced here as an alternative to traditional quantitative structure–activity relationship (QSAR) methods for identifying potential protein mutants with desired properties from minimal sampling of focused libraries. The operation of ASRA depends on identifying the underlying regularity of the protein property landscape, allowing it to make predictions without explicit knowledge of the structure–property relationships. In a proof-of-principle study, ASRA identified all or most of the best enantioselective mutants among the synthesized epoxide hydrolase from Aspergillus niger, in the absence of peptide seeds with high E-values. ASRA even revealed a laboratory error from irregularities of the reordered E-value landscape alone." @default.
- W2097061885 created "2016-06-24" @default.
- W2097061885 creator A5003764862 @default.
- W2097061885 creator A5019365403 @default.
- W2097061885 creator A5050231254 @default.
- W2097061885 creator A5063359273 @default.
- W2097061885 date "2012-03-20" @default.
- W2097061885 modified "2023-10-03" @default.
- W2097061885 title "Enhancing the Efficiency of Directed Evolution in Focused Enzyme Libraries by the Adaptive Substituent Reordering Algorithm" @default.
- W2097061885 cites W1480240092 @default.
- W2097061885 cites W1608623766 @default.
- W2097061885 cites W1898560826 @default.
- W2097061885 cites W1969021085 @default.
- W2097061885 cites W1971850577 @default.
- W2097061885 cites W1972205391 @default.
- W2097061885 cites W1975867035 @default.
- W2097061885 cites W1976547627 @default.
- W2097061885 cites W1980685784 @default.
- W2097061885 cites W1987489304 @default.
- W2097061885 cites W1987696643 @default.
- W2097061885 cites W1988752605 @default.
- W2097061885 cites W1992218202 @default.
- W2097061885 cites W1992447780 @default.
- W2097061885 cites W1993446841 @default.
- W2097061885 cites W1996082096 @default.
- W2097061885 cites W2001952758 @default.
- W2097061885 cites W2003621391 @default.
- W2097061885 cites W2010625545 @default.
- W2097061885 cites W2013090583 @default.
- W2097061885 cites W2015526180 @default.
- W2097061885 cites W2018175631 @default.
- W2097061885 cites W2019998456 @default.
- W2097061885 cites W2021271879 @default.
- W2097061885 cites W2028095555 @default.
- W2097061885 cites W2029213325 @default.
- W2097061885 cites W2031409259 @default.
- W2097061885 cites W2034451231 @default.
- W2097061885 cites W2039262558 @default.
- W2097061885 cites W2040955773 @default.
- W2097061885 cites W2041571635 @default.
- W2097061885 cites W2047015012 @default.
- W2097061885 cites W2060833676 @default.
- W2097061885 cites W2063597962 @default.
- W2097061885 cites W2086462671 @default.
- W2097061885 cites W2089540194 @default.
- W2097061885 cites W2092845657 @default.
- W2097061885 cites W2094826641 @default.
- W2097061885 cites W2095624868 @default.
- W2097061885 cites W2099264016 @default.
- W2097061885 cites W2103385859 @default.
- W2097061885 cites W2103637189 @default.
- W2097061885 cites W2114346581 @default.
- W2097061885 cites W2115737495 @default.
- W2097061885 cites W2118714546 @default.
- W2097061885 cites W2119168556 @default.
- W2097061885 cites W2123687265 @default.
- W2097061885 cites W2134875787 @default.
- W2097061885 cites W2136011546 @default.
- W2097061885 cites W2141346454 @default.
- W2097061885 cites W2142967403 @default.
- W2097061885 cites W2143559788 @default.
- W2097061885 cites W2144794655 @default.
- W2097061885 cites W2148612893 @default.
- W2097061885 cites W2149614036 @default.
- W2097061885 cites W2154277781 @default.
- W2097061885 cites W2154781149 @default.
- W2097061885 cites W2156454061 @default.
- W2097061885 cites W2159848960 @default.
- W2097061885 cites W2170110208 @default.
- W2097061885 cites W4229588781 @default.
- W2097061885 cites W4239603996 @default.
- W2097061885 cites W4239832041 @default.
- W2097061885 cites W4246557136 @default.
- W2097061885 cites W4252488946 @default.
- W2097061885 cites W79383899 @default.
- W2097061885 cites W2013979036 @default.
- W2097061885 doi "https://doi.org/10.1002/chem.201103811" @default.
- W2097061885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22434591" @default.
- W2097061885 hasPublicationYear "2012" @default.
- W2097061885 type Work @default.
- W2097061885 sameAs 2097061885 @default.
- W2097061885 citedByCount "50" @default.
- W2097061885 countsByYear W20970618852012 @default.
- W2097061885 countsByYear W20970618852013 @default.
- W2097061885 countsByYear W20970618852014 @default.
- W2097061885 countsByYear W20970618852015 @default.
- W2097061885 countsByYear W20970618852016 @default.
- W2097061885 countsByYear W20970618852017 @default.
- W2097061885 countsByYear W20970618852018 @default.
- W2097061885 countsByYear W20970618852019 @default.
- W2097061885 countsByYear W20970618852020 @default.
- W2097061885 countsByYear W20970618852021 @default.
- W2097061885 countsByYear W20970618852022 @default.
- W2097061885 countsByYear W20970618852023 @default.
- W2097061885 crossrefType "journal-article" @default.
- W2097061885 hasAuthorship W2097061885A5003764862 @default.
- W2097061885 hasAuthorship W2097061885A5019365403 @default.
- W2097061885 hasAuthorship W2097061885A5050231254 @default.