FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102351707> ?p ?o ?g. }

• W2102351707 endingPage "1419" @default.
• W2102351707 startingPage "1413" @default.
• W2102351707 abstract "ABSTRACT A sensitive, specific, and high-throughput oligonucleotide ligation assay (OLA) for the detection of genotypic human immunodeficiency virus type 1 (HIV-1) resistance to Food and Drug Administration-approved protease inhibitors was developed and evaluated. This ligation-based assay uses differentially modified oligonucleotides specific for wild-type or mutant sequences, allowing sensitive and simple detection of both genotypes in a single well of a microtiter plate. Oligonucleotides were designed to detect primary mutations associated with high-level resistance to amprenavir, nelfinavir, indinavir, ritonavir, saquinavir, and lopinavir, including amino acid substitutions D30N, I50V, V82A/S/T, I84V, N88D, and L90M. Plasma HIV-1 RNA from 54 infected patients was amplified by reverse transcription-PCR and sequenced by using dideoxynucleotide chain terminators for evaluation of mutations associated with drug resistance. These same amplicons were genotyped by the OLA at positions 30, 50, 82, 88, 84, and 90 for a total of 312 codons. The sensitivity of detection of drug-resistant genotypes was 96.7% (87 of 90 mutant codons) in the OLA compared to 92.2% (83 of 90) in consensus sequencing, presumably due to the increased sensitivity of the OLA. The OLA detected genetic subpopulations more often than sequencing, detecting 30 mixtures of mutant and wild-type sequences and two mixtures of drug-resistant sequences compared to 15 detected by DNA sequencing. Reproducible and semiquantitative detection of the mutant and the wild-type genomes by the OLA was observed by analysis of wild-type and mutant plasmid mixtures containing as little as 5% of either genotype in a background of the opposite genome. This rapid, simple, economical, and highly sensitive assay provides a practical alternative to dideoxy sequencing for genotypic evaluation of HIV-1 resistance to antiretrovirals." @default.
• W2102351707 created "2016-06-24" @default.
• W2102351707 creator A5014709746 @default.
• W2102351707 creator A5020991784 @default.
• W2102351707 creator A5029038051 @default.
• W2102351707 creator A5041851449 @default.
• W2102351707 creator A5042424742 @default.
• W2102351707 creator A5052976073 @default.
• W2102351707 creator A5066613720 @default.
• W2102351707 date "2002-04-01" @default.
• W2102351707 modified "2023-10-17" @default.
• W2102351707 title "Rapid and Sensitive Oligonucleotide Ligation Assay for Detection of Mutations in Human Immunodeficiency Virus Type 1 Associated with High-Level Resistance to Protease Inhibitors" @default.
• W2102351707 cites W1524252975 @default.
• W2102351707 cites W1921702285 @default.
• W2102351707 cites W1975606209 @default.
• W2102351707 cites W1980896825 @default.
• W2102351707 cites W1987833086 @default.
• W2102351707 cites W1997844736 @default.
• W2102351707 cites W2025152688 @default.
• W2102351707 cites W2038636949 @default.
• W2102351707 cites W2048627687 @default.
• W2102351707 cites W2068781101 @default.
• W2102351707 cites W2088661249 @default.
• W2102351707 cites W2107303716 @default.
• W2102351707 cites W2107791948 @default.
• W2102351707 cites W2131264611 @default.
• W2102351707 cites W2138042650 @default.
• W2102351707 cites W2142809896 @default.
• W2102351707 cites W2149129111 @default.
• W2102351707 cites W2149335010 @default.
• W2102351707 cites W2153990626 @default.
• W2102351707 cites W2154669635 @default.
• W2102351707 cites W2161916629 @default.
• W2102351707 cites W2163124380 @default.
• W2102351707 cites W2164927340 @default.
• W2102351707 cites W2165770193 @default.
• W2102351707 cites W2166229810 @default.
• W2102351707 cites W2169635828 @default.
• W2102351707 cites W4229706141 @default.
• W2102351707 cites W4238479903 @default.
• W2102351707 cites W4242795671 @default.
• W2102351707 cites W80436974 @default.
• W2102351707 doi "https://doi.org/10.1128/jcm.40.4.1413-1419.2002" @default.
• W2102351707 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/140364" @default.
• W2102351707 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11923366" @default.
• W2102351707 hasPublicationYear "2002" @default.
• W2102351707 type Work @default.
• W2102351707 sameAs 2102351707 @default.
• W2102351707 citedByCount "60" @default.
• W2102351707 countsByYear W21023517072012 @default.
• W2102351707 countsByYear W21023517072013 @default.
• W2102351707 countsByYear W21023517072014 @default.
• W2102351707 countsByYear W21023517072015 @default.
• W2102351707 countsByYear W21023517072016 @default.
• W2102351707 countsByYear W21023517072017 @default.
• W2102351707 countsByYear W21023517072018 @default.
• W2102351707 countsByYear W21023517072019 @default.
• W2102351707 countsByYear W21023517072020 @default.
• W2102351707 countsByYear W21023517072022 @default.
• W2102351707 crossrefType "journal-article" @default.
• W2102351707 hasAuthorship W2102351707A5014709746 @default.
• W2102351707 hasAuthorship W2102351707A5020991784 @default.
• W2102351707 hasAuthorship W2102351707A5029038051 @default.
• W2102351707 hasAuthorship W2102351707A5041851449 @default.
• W2102351707 hasAuthorship W2102351707A5042424742 @default.
• W2102351707 hasAuthorship W2102351707A5052976073 @default.
• W2102351707 hasAuthorship W2102351707A5066613720 @default.
• W2102351707 hasBestOaLocation W21023517072 @default.
• W2102351707 hasConcept C104317684 @default.
• W2102351707 hasConcept C129312508 @default.
• W2102351707 hasConcept C135763542 @default.
• W2102351707 hasConcept C142462285 @default.
• W2102351707 hasConcept C143065580 @default.
• W2102351707 hasConcept C153911025 @default.
• W2102351707 hasConcept C156719811 @default.
• W2102351707 hasConcept C159047783 @default.
• W2102351707 hasConcept C207583985 @default.
• W2102351707 hasConcept C2522874641 @default.
• W2102351707 hasConcept C2776937687 @default.
• W2102351707 hasConcept C2777210460 @default.
• W2102351707 hasConcept C2993143319 @default.
• W2102351707 hasConcept C49105822 @default.
• W2102351707 hasConcept C54355233 @default.
• W2102351707 hasConcept C552990157 @default.
• W2102351707 hasConcept C8185291 @default.
• W2102351707 hasConcept C86803240 @default.
• W2102351707 hasConceptScore W2102351707C104317684 @default.
• W2102351707 hasConceptScore W2102351707C129312508 @default.
• W2102351707 hasConceptScore W2102351707C135763542 @default.
• W2102351707 hasConceptScore W2102351707C142462285 @default.
• W2102351707 hasConceptScore W2102351707C143065580 @default.
• W2102351707 hasConceptScore W2102351707C153911025 @default.
• W2102351707 hasConceptScore W2102351707C156719811 @default.
• W2102351707 hasConceptScore W2102351707C159047783 @default.
• W2102351707 hasConceptScore W2102351707C207583985 @default.
• W2102351707 hasConceptScore W2102351707C2522874641 @default.
• W2102351707 hasConceptScore W2102351707C2776937687 @default.
• W2102351707 hasConceptScore W2102351707C2777210460 @default.

• next