FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2102473268> ?p ?o ?g. }

• W2102473268 endingPage "6570" @default.
• W2102473268 startingPage "6557" @default.
• W2102473268 abstract "Pseudomonas aeruginosa is an opportunistic bacterial pathogen that primarily infects immunocompromised individuals and patients with cystic fibrosis. Invasive strains of P. aeruginosa are known to induce apoptosis at a high frequency in HeLa cells and in many other cell lines, a process that is dependent on the ADP-ribosylation (ADPRT) activity of a type III secreted protein ExoS. In our previous report, it was proposed that P. aeruginosa secreting ExoS, upon infection, shuts down host cell survival signal pathways by inhibiting ERK1/2 and p38 activation, and it activates proapoptotic pathways through activation of JNK1/2, leading ultimately to cytochrome c release and activation of caspases. In this study, we demonstrate that the expression of ExoS in HeLa cells by eukaryotic expression vector effectively caused apoptosis in an ADPRT activity-dependent manner, indicating that ExoS alone is sufficient to trigger apoptotic death of host cells independent of any other bacterial factors. By expressing an EGFP-ExoS fusion protein, we were able to directly correlate the death of HeLa cells with the presence of intracellular ExoS and further proved the dependence of this process on both JNK activation and mitochondrial proapoptotic event. The cellular pathway responsible for the ExoS-induced cytotoxicity appears to be well conserved, since the expression of the ADPRT-competent ExoS also induced rapid cell death in the Drosophila melanogaster S2 cell lines. The presented study not only highlights the ability of ExoS ADPRT to modulate host cell signaling, eventually leading to apoptosis, but also establishes ExoS as a valuable tool, in principle, for the elucidation of apoptosis mechanisms." @default.
• W2102473268 created "2016-06-24" @default.
• W2102473268 creator A5005215310 @default.
• W2102473268 creator A5034651246 @default.
• W2102473268 creator A5036438615 @default.
• W2102473268 creator A5055962801 @default.
• W2102473268 date "2006-12-01" @default.
• W2102473268 modified "2023-10-17" @default.
• W2102473268 title "Expression of <i>Pseudomonas aeruginosa</i> Toxin ExoS Effectively Induces Apoptosis in Host Cells" @default.
• W2102473268 cites W1499653737 @default.
• W2102473268 cites W1505488619 @default.
• W2102473268 cites W1521152447 @default.
• W2102473268 cites W1554372630 @default.
• W2102473268 cites W1596372166 @default.
• W2102473268 cites W1607777232 @default.
• W2102473268 cites W1821042112 @default.
• W2102473268 cites W1834782556 @default.
• W2102473268 cites W1858025662 @default.
• W2102473268 cites W1910528605 @default.
• W2102473268 cites W1929945331 @default.
• W2102473268 cites W1964538721 @default.
• W2102473268 cites W1978372269 @default.
• W2102473268 cites W1980564572 @default.
• W2102473268 cites W1990299056 @default.
• W2102473268 cites W1994480719 @default.
• W2102473268 cites W2005275397 @default.
• W2102473268 cites W2006227243 @default.
• W2102473268 cites W2008532480 @default.
• W2102473268 cites W2009235292 @default.
• W2102473268 cites W2011190624 @default.
• W2102473268 cites W2011847534 @default.
• W2102473268 cites W2015161749 @default.
• W2102473268 cites W2015718294 @default.
• W2102473268 cites W2018531075 @default.
• W2102473268 cites W2019107970 @default.
• W2102473268 cites W2027085093 @default.
• W2102473268 cites W2031368804 @default.
• W2102473268 cites W2031703416 @default.
• W2102473268 cites W2033997567 @default.
• W2102473268 cites W2034401615 @default.
• W2102473268 cites W2036221715 @default.
• W2102473268 cites W2036869422 @default.
• W2102473268 cites W2041923193 @default.
• W2102473268 cites W2043160219 @default.
• W2102473268 cites W2043978867 @default.
• W2102473268 cites W2044722937 @default.
• W2102473268 cites W2046013134 @default.
• W2102473268 cites W2047453903 @default.
• W2102473268 cites W2049284672 @default.
• W2102473268 cites W2053678703 @default.
• W2102473268 cites W2054131802 @default.
• W2102473268 cites W2054270612 @default.
• W2102473268 cites W2061799900 @default.
• W2102473268 cites W2063855381 @default.
• W2102473268 cites W2067659186 @default.
• W2102473268 cites W2068689158 @default.
• W2102473268 cites W2068805793 @default.
• W2102473268 cites W2070847433 @default.
• W2102473268 cites W2072439886 @default.
• W2102473268 cites W2075185464 @default.
• W2102473268 cites W2078262516 @default.
• W2102473268 cites W2079427461 @default.
• W2102473268 cites W2083861520 @default.
• W2102473268 cites W2085609763 @default.
• W2102473268 cites W2091066588 @default.
• W2102473268 cites W2094038241 @default.
• W2102473268 cites W2094761758 @default.
• W2102473268 cites W2096797367 @default.
• W2102473268 cites W2105736223 @default.
• W2102473268 cites W2109009084 @default.
• W2102473268 cites W2110230880 @default.
• W2102473268 cites W2113061812 @default.
• W2102473268 cites W2113666896 @default.
• W2102473268 cites W2114770722 @default.
• W2102473268 cites W2115389096 @default.
• W2102473268 cites W2115555723 @default.
• W2102473268 cites W2118126255 @default.
• W2102473268 cites W2119236186 @default.
• W2102473268 cites W2119679614 @default.
• W2102473268 cites W2121851559 @default.
• W2102473268 cites W2122351989 @default.
• W2102473268 cites W2125963106 @default.
• W2102473268 cites W2127567657 @default.
• W2102473268 cites W2128822902 @default.
• W2102473268 cites W2132417094 @default.
• W2102473268 cites W2135733854 @default.
• W2102473268 cites W2135782606 @default.
• W2102473268 cites W2137749186 @default.
• W2102473268 cites W2144361833 @default.
• W2102473268 cites W2144878722 @default.
• W2102473268 cites W2145626047 @default.
• W2102473268 cites W2149967205 @default.
• W2102473268 cites W2150685019 @default.
• W2102473268 cites W2155228106 @default.
• W2102473268 cites W2156191230 @default.
• W2102473268 cites W2156297388 @default.
• W2102473268 cites W2158102238 @default.
• W2102473268 cites W2159450084 @default.

• next