FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103446563> ?p ?o ?g. }

• W2103446563 endingPage "e24541" @default.
• W2103446563 startingPage "e24541" @default.
• W2103446563 abstract "Type 1 iodothyronine deiodinase (DIO1) catalyses the conversion of prohormone thyroxine to the active thyroid hormone 3,3′,5-triiodothyronine (T3), important regulator of cell proliferation and differentiation. DIO1 expression is reduced in the most common type of kidney neoplasia, clear cell Renal Cell Carcinoma (ccRCC). MicroRNAs are small, non-coding RNAs that regulate gene expression at posttranscriptional levels. The aim of this study was to analyze the potential regulation of DIO1 expression by microRNAs in ccRCC. Bioinformatic analysis revealed that 3′UTR of the human DIO1 gene transcript contains miR-224 and miR-383 target sites, which are conserved across mammalian species. Semi-quantitative real-time PCR was used to analyze the expression of miR-224 and miR-383 in 32 samples of ccRCC tumors (T) and in 32 matched control (C) samples. We observed statistically significant (p = 0.0002) more than four fold increase in miR-224 expression and nearly two fold increase in miR-383 expression in samples T compared to samples C. Tumor specific changes in expression of miR-224 negatively correlated with changes in DIO1 expression and intracellular T3 concentration. Transfection of HeLa cell line with miR-224 and miR-383 suppressed the activity of a luciferase reporter containing the 3′UTR of DIO1. This was abolished when constructs mutated at the miR-224 and miR-383 target sites were used instead, indicating that miR-224 and miR-383 directly bind to DIO1 3′UTR. Finally, induced expression of miR-224 in Caki-2 cells resulted in significant (p<0.01) reduction of DIO1 mRNA. This study provides a novel miRNA-mediated regulatory mechanism of DIO1 expression in ccRCC." @default.
• W2103446563 created "2016-06-24" @default.
• W2103446563 creator A5004205839 @default.
• W2103446563 creator A5039335227 @default.
• W2103446563 creator A5039536569 @default.
• W2103446563 creator A5053975368 @default.
• W2103446563 creator A5090743345 @default.
• W2103446563 date "2011-09-02" @default.
• W2103446563 modified "2023-10-16" @default.
• W2103446563 title "MiR-224 Targets the 3′UTR of Type 1 5′-Iodothyronine Deiodinase Possibly Contributing to Tissue Hypothyroidism in Renal Cancer" @default.
• W2103446563 cites W144423133 @default.
• W2103446563 cites W1648301895 @default.
• W2103446563 cites W1967342065 @default.
• W2103446563 cites W1968349455 @default.
• W2103446563 cites W1969213466 @default.
• W2103446563 cites W1972755526 @default.
• W2103446563 cites W1978337436 @default.
• W2103446563 cites W1984310354 @default.
• W2103446563 cites W1985402095 @default.
• W2103446563 cites W1985405072 @default.
• W2103446563 cites W1989781457 @default.
• W2103446563 cites W1992272392 @default.
• W2103446563 cites W1993080614 @default.
• W2103446563 cites W1999787565 @default.
• W2103446563 cites W2002995168 @default.
• W2103446563 cites W2009279040 @default.
• W2103446563 cites W2009280755 @default.
• W2103446563 cites W2013411684 @default.
• W2103446563 cites W2016112105 @default.
• W2103446563 cites W2022230607 @default.
• W2103446563 cites W2030120875 @default.
• W2103446563 cites W2036169252 @default.
• W2103446563 cites W2036224487 @default.
• W2103446563 cites W2039164708 @default.
• W2103446563 cites W2040191060 @default.
• W2103446563 cites W2041353175 @default.
• W2103446563 cites W2048603557 @default.
• W2103446563 cites W2050869737 @default.
• W2103446563 cites W2059073003 @default.
• W2103446563 cites W2064544204 @default.
• W2103446563 cites W2068138819 @default.
• W2103446563 cites W2070571588 @default.
• W2103446563 cites W2074415792 @default.
• W2103446563 cites W2074472120 @default.
• W2103446563 cites W2075679644 @default.
• W2103446563 cites W2081070032 @default.
• W2103446563 cites W2091651379 @default.
• W2103446563 cites W2099908487 @default.
• W2103446563 cites W2104878795 @default.
• W2103446563 cites W2107277218 @default.
• W2103446563 cites W2109136664 @default.
• W2103446563 cites W2109458276 @default.
• W2103446563 cites W2110892195 @default.
• W2103446563 cites W2118929134 @default.
• W2103446563 cites W2122211092 @default.
• W2103446563 cites W2123680400 @default.
• W2103446563 cites W2125523949 @default.
• W2103446563 cites W2127826417 @default.
• W2103446563 cites W2130281728 @default.
• W2103446563 cites W2135836598 @default.
• W2103446563 cites W2135958288 @default.
• W2103446563 cites W2140186164 @default.
• W2103446563 cites W2144038313 @default.
• W2103446563 cites W2150536104 @default.
• W2103446563 cites W2154582555 @default.
• W2103446563 cites W2159793493 @default.
• W2103446563 cites W2159888101 @default.
• W2103446563 cites W2171201030 @default.
• W2103446563 doi "https://doi.org/10.1371/journal.pone.0024541" @default.
• W2103446563 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3166326" @default.
• W2103446563 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21912701" @default.
• W2103446563 hasPublicationYear "2011" @default.
• W2103446563 type Work @default.
• W2103446563 sameAs 2103446563 @default.
• W2103446563 citedByCount "71" @default.
• W2103446563 countsByYear W21034465632012 @default.
• W2103446563 countsByYear W21034465632013 @default.
• W2103446563 countsByYear W21034465632014 @default.
• W2103446563 countsByYear W21034465632015 @default.
• W2103446563 countsByYear W21034465632016 @default.
• W2103446563 countsByYear W21034465632017 @default.
• W2103446563 countsByYear W21034465632018 @default.
• W2103446563 countsByYear W21034465632019 @default.
• W2103446563 countsByYear W21034465632020 @default.
• W2103446563 countsByYear W21034465632021 @default.
• W2103446563 countsByYear W21034465632022 @default.
• W2103446563 countsByYear W21034465632023 @default.
• W2103446563 crossrefType "journal-article" @default.
• W2103446563 hasAuthorship W2103446563A5004205839 @default.
• W2103446563 hasAuthorship W2103446563A5039335227 @default.
• W2103446563 hasAuthorship W2103446563A5039536569 @default.
• W2103446563 hasAuthorship W2103446563A5053975368 @default.
• W2103446563 hasAuthorship W2103446563A5090743345 @default.
• W2103446563 hasBestOaLocation W21034465631 @default.
• W2103446563 hasConcept C104317684 @default.
• W2103446563 hasConcept C105580179 @default.
• W2103446563 hasConcept C111335760 @default.
• W2103446563 hasConcept C126322002 @default.

• next