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- W2109012121 abstract "miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This repressed set of genes significantly overlaps with miRNA-1 regulated genes that have been identified with DNA array technology and are predicted by computational methods. Moreover, we find that the 3′-untranslated region for the repressed set are enriched in miRNA-1 complementary sites. Our findings demonstrate that SILAC can be used for miRNA target identification and that one highly expressed miRNA can regulate the levels of many different proteins." @default.
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- W2109012121 date "2006-08-31" @default.
- W2109012121 modified "2023-10-09" @default.
- W2109012121 title "Identification of miRNA targets with stable isotope labeling by amino acids in cell culture" @default.
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- W2109012121 doi "https://doi.org/10.1093/nar/gkl590" @default.
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