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• W2111417918 abstract "Manufacturing of bioactive cell culture substrates represents a major challenge for the development of cell therapy for tissue repair and immune treatment of cancers, infectious diseases, or immunodeficiencies. In this context, we evaluated the capacity of several differentiation factors, including Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF), to drive differentiation of primary cell cultures, once immobilized on surfaces. We show that covalently immobilized signal factors fully retain their biological properties and efficiently promote differentiation of mouse and/or human precursor cells leading to the production of dendritic cells and macrophages. For GM-CSF, we also show that the efficiency of receptor signaling is comparable using either soluble or tethered molecules. Such artificial bioactive interfaces are suitable for the development and automated production of cell-based vaccines and therapies." @default.
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• W2111417918 date "2008-09-15" @default.
• W2111417918 modified "2023-10-16" @default.
• W2111417918 title "Immobilized cytokines as biomaterials for manufacturing immune cell based vaccines" @default.
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• W2111417918 doi "https://doi.org/10.1002/jbm.a.31751" @default.
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