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• W2124306486 abstract "We have developed an expert system that makes use of various kinds of knowledge organized as if-then rules for predicting protein localization sites in Gram-negative bacteria, given the amino acid sequence information alone. We considered four localization sites: the cytoplasm, the inner (cytoplasmic) membrane, the periplasm, and the outer membrane. Most rules were derived from experimental observations. For example, the rule to recognize an inner membrane protein is the presence of either a hydrophobic stretch in the predicted mature protein or an uncleavable N-terminal signal sequence. Lipoproteins are first recognized by a consensus pattern and then assumed present at either the inner or outer membrane. These two possibilities are further discriminated by examining an acidic residue in the mature N-terminal portion. Furthermore, we found an empirical rule that periplasmic and outer membrane proteins were successfully discriminated by their different amino acid composition. Overall, our system could predict 83% of the localization sites of proteins in our database." @default.
• W2124306486 created "2016-06-24" @default.
• W2124306486 creator A5054681585 @default.
• W2124306486 creator A5058022821 @default.
• W2124306486 date "1991-10-01" @default.
• W2124306486 modified "2023-10-13" @default.
• W2124306486 title "Expert system for predicting protein localization sites in gram-negative bacteria" @default.
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• W2124306486 doi "https://doi.org/10.1002/prot.340110203" @default.
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