FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145013669> ?p ?o ?g. }

• W2145013669 endingPage "S175" @default.
• W2145013669 startingPage "S175" @default.
• W2145013669 abstract "ObjectiveDirect visualization of meiotic exchanges in chromosomally normal and abnormal human oocytes was completed to conduct detailed studies on the frequency, distribution, and placement of recombination sites in aneuploid and euploid gametes.Study designOocytes from normal fetuses and a 20 week trisomy 18 fetus underwent fluorescence antibody staining (FAS) with meiotic proteins associated with synaptonemal complex formation (SCP3) and the DNA mismatch repair protein, MLH 1, that locates recombination foci. Fluoresence in situ hybridization (FISH) was completed to evaluate for chromosome-specific variations in exchange patterns.ResultsMLH 1 foci were present from zygotene through diplotene in chromosomally normal and abnormal meiocytes. 32 trisomy 18 and 37 chromosomally normal pachytene stage meiocytes were studied for recombination characteristics. The mean number of MLH1 foci, 39, was lower in the trisomy 18 gametes compared to 69 for the chromosomally normal gametes. MLH 1 foci location per chromosome arm on chromosomes other than chromosome 18 was similar to that of chromosomally normal oocytes. Interference was observed.ConclusionThis is the first report on the recombination pattern of human trisomic meiocytes. The findings suggest that recombination is disturbed and deficient in trisomic oocytes. Such disturbances might be a risk factor for oocyte atresia and could be explain the observed ovarian dysgenesis seen in trisomy 18 infants. Reduced recombination may also increase the risk for abnormal chromosome segregation leading to aneuploid gametes and provide a mechanism to explain the interchromosomal effect. ObjectiveDirect visualization of meiotic exchanges in chromosomally normal and abnormal human oocytes was completed to conduct detailed studies on the frequency, distribution, and placement of recombination sites in aneuploid and euploid gametes. Direct visualization of meiotic exchanges in chromosomally normal and abnormal human oocytes was completed to conduct detailed studies on the frequency, distribution, and placement of recombination sites in aneuploid and euploid gametes. Study designOocytes from normal fetuses and a 20 week trisomy 18 fetus underwent fluorescence antibody staining (FAS) with meiotic proteins associated with synaptonemal complex formation (SCP3) and the DNA mismatch repair protein, MLH 1, that locates recombination foci. Fluoresence in situ hybridization (FISH) was completed to evaluate for chromosome-specific variations in exchange patterns. Oocytes from normal fetuses and a 20 week trisomy 18 fetus underwent fluorescence antibody staining (FAS) with meiotic proteins associated with synaptonemal complex formation (SCP3) and the DNA mismatch repair protein, MLH 1, that locates recombination foci. Fluoresence in situ hybridization (FISH) was completed to evaluate for chromosome-specific variations in exchange patterns. ResultsMLH 1 foci were present from zygotene through diplotene in chromosomally normal and abnormal meiocytes. 32 trisomy 18 and 37 chromosomally normal pachytene stage meiocytes were studied for recombination characteristics. The mean number of MLH1 foci, 39, was lower in the trisomy 18 gametes compared to 69 for the chromosomally normal gametes. MLH 1 foci location per chromosome arm on chromosomes other than chromosome 18 was similar to that of chromosomally normal oocytes. Interference was observed. MLH 1 foci were present from zygotene through diplotene in chromosomally normal and abnormal meiocytes. 32 trisomy 18 and 37 chromosomally normal pachytene stage meiocytes were studied for recombination characteristics. The mean number of MLH1 foci, 39, was lower in the trisomy 18 gametes compared to 69 for the chromosomally normal gametes. MLH 1 foci location per chromosome arm on chromosomes other than chromosome 18 was similar to that of chromosomally normal oocytes. Interference was observed. ConclusionThis is the first report on the recombination pattern of human trisomic meiocytes. The findings suggest that recombination is disturbed and deficient in trisomic oocytes. Such disturbances might be a risk factor for oocyte atresia and could be explain the observed ovarian dysgenesis seen in trisomy 18 infants. Reduced recombination may also increase the risk for abnormal chromosome segregation leading to aneuploid gametes and provide a mechanism to explain the interchromosomal effect. This is the first report on the recombination pattern of human trisomic meiocytes. The findings suggest that recombination is disturbed and deficient in trisomic oocytes. Such disturbances might be a risk factor for oocyte atresia and could be explain the observed ovarian dysgenesis seen in trisomy 18 infants. Reduced recombination may also increase the risk for abnormal chromosome segregation leading to aneuploid gametes and provide a mechanism to explain the interchromosomal effect." @default.
• W2145013669 created "2016-06-24" @default.
• W2145013669 creator A5007648942 @default.
• W2145013669 creator A5007699479 @default.
• W2145013669 creator A5026776942 @default.
• W2145013669 creator A5035082427 @default.
• W2145013669 creator A5076206465 @default.
• W2145013669 date "2006-12-01" @default.
• W2145013669 modified "2023-10-16" @default.
• W2145013669 title "Direct analysis of meiotic recombination in human trisomy 18 oocytes" @default.
• W2145013669 doi "https://doi.org/10.1016/j.ajog.2006.10.622" @default.
• W2145013669 hasPublicationYear "2006" @default.
• W2145013669 type Work @default.
• W2145013669 sameAs 2145013669 @default.
• W2145013669 citedByCount "0" @default.
• W2145013669 crossrefType "journal-article" @default.
• W2145013669 hasAuthorship W2145013669A5007648942 @default.
• W2145013669 hasAuthorship W2145013669A5007699479 @default.
• W2145013669 hasAuthorship W2145013669A5026776942 @default.
• W2145013669 hasAuthorship W2145013669A5035082427 @default.
• W2145013669 hasAuthorship W2145013669A5076206465 @default.
• W2145013669 hasConcept C102744134 @default.
• W2145013669 hasConcept C104317684 @default.
• W2145013669 hasConcept C148196450 @default.
• W2145013669 hasConcept C16685009 @default.
• W2145013669 hasConcept C2776229224 @default.
• W2145013669 hasConcept C2777542201 @default.
• W2145013669 hasConcept C2779672484 @default.
• W2145013669 hasConcept C30481170 @default.
• W2145013669 hasConcept C54059333 @default.
• W2145013669 hasConcept C54355233 @default.
• W2145013669 hasConcept C552990157 @default.
• W2145013669 hasConcept C71924100 @default.
• W2145013669 hasConcept C86803240 @default.
• W2145013669 hasConceptScore W2145013669C102744134 @default.
• W2145013669 hasConceptScore W2145013669C104317684 @default.
• W2145013669 hasConceptScore W2145013669C148196450 @default.
• W2145013669 hasConceptScore W2145013669C16685009 @default.
• W2145013669 hasConceptScore W2145013669C2776229224 @default.
• W2145013669 hasConceptScore W2145013669C2777542201 @default.
• W2145013669 hasConceptScore W2145013669C2779672484 @default.
• W2145013669 hasConceptScore W2145013669C30481170 @default.
• W2145013669 hasConceptScore W2145013669C54059333 @default.
• W2145013669 hasConceptScore W2145013669C54355233 @default.
• W2145013669 hasConceptScore W2145013669C552990157 @default.
• W2145013669 hasConceptScore W2145013669C71924100 @default.
• W2145013669 hasConceptScore W2145013669C86803240 @default.
• W2145013669 hasIssue "6" @default.
• W2145013669 hasLocation W21450136691 @default.
• W2145013669 hasOpenAccess W2145013669 @default.
• W2145013669 hasPrimaryLocation W21450136691 @default.
• W2145013669 hasRelatedWork W1967762412 @default.
• W2145013669 hasRelatedWork W1971479618 @default.
• W2145013669 hasRelatedWork W2004955435 @default.
• W2145013669 hasRelatedWork W2009777273 @default.
• W2145013669 hasRelatedWork W2057965747 @default.
• W2145013669 hasRelatedWork W2086932293 @default.
• W2145013669 hasRelatedWork W2106430201 @default.
• W2145013669 hasRelatedWork W2120547798 @default.
• W2145013669 hasRelatedWork W2145013669 @default.
• W2145013669 hasRelatedWork W2092874662 @default.
• W2145013669 hasVolume "195" @default.
• W2145013669 isParatext "false" @default.
• W2145013669 isRetracted "false" @default.
• W2145013669 magId "2145013669" @default.
• W2145013669 workType "article" @default.