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• W2146173892 abstract "The establishment of patient-specific histocompatible stem cells may be an alternative for overcoming current limitations in stem cell engineering. We are developing an animal model to assist the establishment of histocompatible, autologous stem cells. In this process, we obtained valuable information on establishing and characterizing stem cells. As an initial step, we succeeded in establishing histocompatible stem cells using preantral follicle cultures and subsequent parthenogenetic activation. The gene expression profile of the established stem cells was similar to that of embryonic stem cells (ESCs) derived from normal fertilization. On the other hand, we propose a way to derive histocompatible, ESC-like cells by co-culturing ovarian stromal cells with feeder fibroblasts, which may allow the derivation of stem cells from somatic tissue. However, more progress regarding the establishment and elucidation on origination of established cell lines is necessary to use this genetic manipulation-free procedure. Nevertheless, relevant information on the process will help to stimulate preclinical research on cell transformation into differentiated, undifferentiated, and even cancerous cells, as well as clinical studies on the application of induced pluripotent cells." @default.
• W2146173892 created "2016-06-24" @default.
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• W2146173892 date "2011-06-03" @default.
• W2146173892 modified "2023-09-26" @default.
• W2146173892 title "Genetic and cellular aspects of the establishment of histocompatible stem cells: information gained from an animal model" @default.
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• W2146173892 doi "https://doi.org/10.1186/1753-6561-5-s4-s31" @default.
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