FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2149278869> ?p ?o ?g. }

• W2149278869 endingPage "2677" @default.
• W2149278869 startingPage "2671" @default.
• W2149278869 abstract "ABSTRACT The Francisella tularensis -containing phagosome (FCP) matures to a late-endosome-like phagosome prior to bacterial escape into the cytosols of macrophages, where bacterial proliferation occurs. Our data show that within the first 15 min after infection of primary human monocyte-derived macrophages (hMDMs), ∼90% of the FCPs acquire the proton vacuolar ATPase (vATPase) pump and the lysomotropic dye LysoTracker, which concentrates in acidic compartments, similar to phagosomes harboring the Listeria monocytogenes control. The acquired proton vATPase pump and lysomotropic dye are gradually lost by 30 to 60 min postinfection, which coincides with bacterial escape into the cytosols of hMDMs. Colocalization of phagosomes harboring the iglD mutant with the vATPase pump and the LysoTracker dye was also transient, and the loss of colocalization was faster than that observed for the wild-type strain, which is consistent with the faster escape of the iglD mutant into the macrophage cytosol. In contrast, colocalization of both makers with phagosomes harboring the iglC mutant was persistent, which is consistent with fusion to the lysosomes and failure of the iglC mutant to escape into the macrophage cytosol. We have utilized a fluorescence microscopy-based phagosome integrity assay for differential labeling of vacuolar versus cytosolic bacteria, using antibacterial antibodies loaded into the cytosols of live hMDMs. We show that specific inhibition of the proton vATPase pump by bafilomycin A1 (BFA) blocks rapid bacterial escape into the cytosols of hMDMs, but 30% to 50% of the bacteria escape into the cytosol by 6 to 12 h after BFA treatment. The effect of BFA on the blocking of bacterial escape into the cytosol is completely reversible, as the bacteria escape after removal of BFA. We also show that the limited fusion of the FCP to lysosomes is not due to failure to recruit the late-endosomal fusion regulator Rab7. Therefore, within few minutes of its biogenesis, the FCP transiently acquires the proton vATPase pump to acidify the phagosome, and this transient acidification is essential for subsequent bacterial escape into the macrophage cytosol." @default.
• W2149278869 created "2016-06-24" @default.
• W2149278869 creator A5018120874 @default.
• W2149278869 creator A5019512112 @default.
• W2149278869 creator A5027696454 @default.
• W2149278869 creator A5053228134 @default.
• W2149278869 creator A5083614444 @default.
• W2149278869 date "2008-06-01" @default.
• W2149278869 modified "2023-10-17" @default.
• W2149278869 title "Acquisition of the Vacuolar ATPase Proton Pump and Phagosome Acidification Are Essential for Escape of <i>Francisella tularensis</i> into the Macrophage Cytosol" @default.
• W2149278869 cites W1501109059 @default.
• W2149278869 cites W1740020315 @default.
• W2149278869 cites W1901426168 @default.
• W2149278869 cites W1916974759 @default.
• W2149278869 cites W1967378722 @default.
• W2149278869 cites W1968769252 @default.
• W2149278869 cites W1971115393 @default.
• W2149278869 cites W1972506258 @default.
• W2149278869 cites W1999795482 @default.
• W2149278869 cites W2001105170 @default.
• W2149278869 cites W2005719423 @default.
• W2149278869 cites W2022537334 @default.
• W2149278869 cites W2035501441 @default.
• W2149278869 cites W2037307064 @default.
• W2149278869 cites W2037559791 @default.
• W2149278869 cites W2037745853 @default.
• W2149278869 cites W2040871602 @default.
• W2149278869 cites W2093725577 @default.
• W2149278869 cites W2098798252 @default.
• W2149278869 cites W2105736821 @default.
• W2149278869 cites W2111593103 @default.
• W2149278869 cites W2143969661 @default.
• W2149278869 cites W2154933996 @default.
• W2149278869 cites W2166802926 @default.
• W2149278869 cites W2169132563 @default.
• W2149278869 cites W2170163435 @default.
• W2149278869 cites W4211067865 @default.
• W2149278869 doi "https://doi.org/10.1128/iai.00185-08" @default.
• W2149278869 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2423071" @default.
• W2149278869 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18390995" @default.
• W2149278869 hasPublicationYear "2008" @default.
• W2149278869 type Work @default.
• W2149278869 sameAs 2149278869 @default.
• W2149278869 citedByCount "113" @default.
• W2149278869 countsByYear W21492788692012 @default.
• W2149278869 countsByYear W21492788692013 @default.
• W2149278869 countsByYear W21492788692014 @default.
• W2149278869 countsByYear W21492788692015 @default.
• W2149278869 countsByYear W21492788692016 @default.
• W2149278869 countsByYear W21492788692017 @default.
• W2149278869 countsByYear W21492788692018 @default.
• W2149278869 countsByYear W21492788692020 @default.
• W2149278869 countsByYear W21492788692021 @default.
• W2149278869 countsByYear W21492788692022 @default.
• W2149278869 countsByYear W21492788692023 @default.
• W2149278869 crossrefType "journal-article" @default.
• W2149278869 hasAuthorship W2149278869A5018120874 @default.
• W2149278869 hasAuthorship W2149278869A5019512112 @default.
• W2149278869 hasAuthorship W2149278869A5027696454 @default.
• W2149278869 hasAuthorship W2149278869A5053228134 @default.
• W2149278869 hasAuthorship W2149278869A5083614444 @default.
• W2149278869 hasBestOaLocation W21492788691 @default.
• W2149278869 hasConcept C102568950 @default.
• W2149278869 hasConcept C102747710 @default.
• W2149278869 hasConcept C104317684 @default.
• W2149278869 hasConcept C160448771 @default.
• W2149278869 hasConcept C161478664 @default.
• W2149278869 hasConcept C181199279 @default.
• W2149278869 hasConcept C190062978 @default.
• W2149278869 hasConcept C190283241 @default.
• W2149278869 hasConcept C202751555 @default.
• W2149278869 hasConcept C203522944 @default.
• W2149278869 hasConcept C23265538 @default.
• W2149278869 hasConcept C2777722557 @default.
• W2149278869 hasConcept C2779244956 @default.
• W2149278869 hasConcept C2780467176 @default.
• W2149278869 hasConcept C2780796267 @default.
• W2149278869 hasConcept C40209533 @default.
• W2149278869 hasConcept C55493867 @default.
• W2149278869 hasConcept C60987743 @default.
• W2149278869 hasConcept C79879829 @default.
• W2149278869 hasConcept C86803240 @default.
• W2149278869 hasConcept C89423630 @default.
• W2149278869 hasConcept C95444343 @default.
• W2149278869 hasConcept C98539663 @default.
• W2149278869 hasConceptScore W2149278869C102568950 @default.
• W2149278869 hasConceptScore W2149278869C102747710 @default.
• W2149278869 hasConceptScore W2149278869C104317684 @default.
• W2149278869 hasConceptScore W2149278869C160448771 @default.
• W2149278869 hasConceptScore W2149278869C161478664 @default.
• W2149278869 hasConceptScore W2149278869C181199279 @default.
• W2149278869 hasConceptScore W2149278869C190062978 @default.
• W2149278869 hasConceptScore W2149278869C190283241 @default.
• W2149278869 hasConceptScore W2149278869C202751555 @default.
• W2149278869 hasConceptScore W2149278869C203522944 @default.
• W2149278869 hasConceptScore W2149278869C23265538 @default.
• W2149278869 hasConceptScore W2149278869C2777722557 @default.
• W2149278869 hasConceptScore W2149278869C2779244956 @default.

• next