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- W2152354512 abstract "The relative simplicity of the in vitro micronucleus (MNvit) endpoint has made it amenable to several automated scoring approaches. Flow cytometry is one such scoring platform that has been successfully employed. This review describes the origins of the MNvit assay, as well as the evolution and properties of flow cytometry-based scoring systems. While the current state-of-the-art methods acquire micronucleus (MN) frequency data very efficiently, it is becoming clear that they also endow the assay with high information content. For instance, simultaneous with MN frequency determinations, several additional endpoints are acquired that provide insights into cytotoxicity, cell cycle perturbations and, in the event of MN induction, information about genotoxic mode of action. This review concludes with a discussion regarding data gaps and also recommendations for additional work that is needed to more fully realise the potential of flow cytometric MNvit scoring." @default.
- W2152354512 created "2016-06-24" @default.
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- W2152354512 date "2010-12-16" @default.
- W2152354512 modified "2023-10-06" @default.
- W2152354512 title "Flow cytometric analysis of micronuclei in mammalian cell cultures: past, present and future" @default.
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- W2152354512 doi "https://doi.org/10.1093/mutage/geq058" @default.
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