FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2156816825> ?p ?o ?g. }

• W2156816825 endingPage "166" @default.
• W2156816825 startingPage "158" @default.
• W2156816825 abstract "Aim: The endocannabinoid (EC) system is a major component in the control of energy homeostasis. It mediates a positive energy balance via central and peripheral pathways. Blockade of the cannabinoid type 1 receptor induces weight reduction and improves cardiovascular risk factors in overweight patients. Cannabinoid receptor type 1 (CB1R)-deficient mice are resistant to diet-induced obesity. The mechanisms responsible for these effects remain only partially elucidated. We hypothesized peripheral effects via direct modulation of adipocyte function to be an integral part of EC action on energy metabolism and insulin sensitivity. Methods: SV40 immortalized murine white and brown adipocytes were used for all experiments. We investigated the effect of CB1R blockade by stimulating the cells acutely and chronically with rimonabant, a selective antagonist for the CB1R, or by knocking down the receptor with small interfering RNA (siRNA). Changes in thermogenic mRNA and protein expression as well as mitochondrial biogenesis and function were assessed by real-time RT-PCR, immunoblotting, fluorescent staining techniques, electron microscopy and by measuring oxygen consumption. Results: Acute and chronic blockade of the CB1R with the selective antagonist rimonabant or by siRNA in murine white adipocytes strongly induced the thermogenic uncoupling protein-1 (UCP-1). UCP-1 expression was increased in a time- and dose-dependent manner both at the RNA and protein level. Furthermore, this effect was paralleled by enhanced peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) expression. In accordance with these findings, AMP-activated protein kinase (AMPK) phosphorylation was also increased after rimonabant treatment. Mitochondria-specific fluorescent staining demonstrated an augmentation in the number of mitochondria. This was confirmed by electron microscopy images. Moreover, rimonabant treatment enhanced the cytochrome c oxidase activity and increased cellular oxygen consumption. Conclusions: Taken together, our data demonstrate that inhibition of peripheral CB1R action in adipocytes directly promotes transdifferentiation of white adipocytes into a mitochondria-rich, thermogenic brown fat phenotype. Enhanced thermogenesis and insulin sensitivity may represent a peripheral mechanism contributing to weight loss and improved glucose homeostasis in rimonabant-treated patients." @default.
• W2156816825 created "2016-06-24" @default.
• W2156816825 creator A5012662187 @default.
• W2156816825 creator A5017198489 @default.
• W2156816825 creator A5030486669 @default.
• W2156816825 creator A5034238429 @default.
• W2156816825 creator A5038694220 @default.
• W2156816825 creator A5047183170 @default.
• W2156816825 creator A5049238642 @default.
• W2156816825 creator A5054296137 @default.
• W2156816825 creator A5070664858 @default.
• W2156816825 creator A5079250860 @default.
• W2156816825 date "2010-02-01" @default.
• W2156816825 modified "2023-09-27" @default.
• W2156816825 title "Cannabinoid type 1 receptor blockade induces transdifferentiation towards a brown fat phenotype in white adipocytes" @default.
• W2156816825 cites W1503600099 @default.
• W2156816825 cites W1970140633 @default.
• W2156816825 cites W1971698096 @default.
• W2156816825 cites W1975370202 @default.
• W2156816825 cites W1976416429 @default.
• W2156816825 cites W1978777057 @default.
• W2156816825 cites W1981147834 @default.
• W2156816825 cites W1988848089 @default.
• W2156816825 cites W1991778481 @default.
• W2156816825 cites W1992114028 @default.
• W2156816825 cites W1992794749 @default.
• W2156816825 cites W2000219716 @default.
• W2156816825 cites W2003425100 @default.
• W2156816825 cites W2008632158 @default.
• W2156816825 cites W2039992465 @default.
• W2156816825 cites W2044969295 @default.
• W2156816825 cites W2068986819 @default.
• W2156816825 cites W2073734463 @default.
• W2156816825 cites W2077206745 @default.
• W2156816825 cites W2077914233 @default.
• W2156816825 cites W2081848794 @default.
• W2156816825 cites W2087587748 @default.
• W2156816825 cites W2091315563 @default.
• W2156816825 cites W2099249565 @default.
• W2156816825 cites W2100002091 @default.
• W2156816825 cites W2106934282 @default.
• W2156816825 cites W2114978842 @default.
• W2156816825 cites W2138121830 @default.
• W2156816825 cites W2147023009 @default.
• W2156816825 cites W2149752088 @default.
• W2156816825 cites W2154979992 @default.
• W2156816825 cites W2161687990 @default.
• W2156816825 cites W2165284792 @default.
• W2156816825 doi "https://doi.org/10.1111/j.1463-1326.2009.01133.x" @default.
• W2156816825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19895638" @default.
• W2156816825 hasPublicationYear "2010" @default.
• W2156816825 type Work @default.
• W2156816825 sameAs 2156816825 @default.
• W2156816825 citedByCount "92" @default.
• W2156816825 countsByYear W21568168252012 @default.
• W2156816825 countsByYear W21568168252013 @default.
• W2156816825 countsByYear W21568168252014 @default.
• W2156816825 countsByYear W21568168252015 @default.
• W2156816825 countsByYear W21568168252016 @default.
• W2156816825 countsByYear W21568168252017 @default.
• W2156816825 countsByYear W21568168252018 @default.
• W2156816825 countsByYear W21568168252019 @default.
• W2156816825 countsByYear W21568168252020 @default.
• W2156816825 countsByYear W21568168252021 @default.
• W2156816825 countsByYear W21568168252022 @default.
• W2156816825 countsByYear W21568168252023 @default.
• W2156816825 crossrefType "journal-article" @default.
• W2156816825 hasAuthorship W2156816825A5012662187 @default.
• W2156816825 hasAuthorship W2156816825A5017198489 @default.
• W2156816825 hasAuthorship W2156816825A5030486669 @default.
• W2156816825 hasAuthorship W2156816825A5034238429 @default.
• W2156816825 hasAuthorship W2156816825A5038694220 @default.
• W2156816825 hasAuthorship W2156816825A5047183170 @default.
• W2156816825 hasAuthorship W2156816825A5049238642 @default.
• W2156816825 hasAuthorship W2156816825A5054296137 @default.
• W2156816825 hasAuthorship W2156816825A5070664858 @default.
• W2156816825 hasAuthorship W2156816825A5079250860 @default.
• W2156816825 hasConcept C126322002 @default.
• W2156816825 hasConcept C134018914 @default.
• W2156816825 hasConcept C143128242 @default.
• W2156816825 hasConcept C148001335 @default.
• W2156816825 hasConcept C151955695 @default.
• W2156816825 hasConcept C170493617 @default.
• W2156816825 hasConcept C171089720 @default.
• W2156816825 hasConcept C2776433454 @default.
• W2156816825 hasConcept C2776885963 @default.
• W2156816825 hasConcept C2777229759 @default.
• W2156816825 hasConcept C2779406938 @default.
• W2156816825 hasConcept C2780642333 @default.
• W2156816825 hasConcept C2780871563 @default.
• W2156816825 hasConcept C28859421 @default.
• W2156816825 hasConcept C46721173 @default.
• W2156816825 hasConcept C71924100 @default.
• W2156816825 hasConcept C81979416 @default.
• W2156816825 hasConcept C86803240 @default.
• W2156816825 hasConcept C95444343 @default.
• W2156816825 hasConceptScore W2156816825C126322002 @default.
• W2156816825 hasConceptScore W2156816825C134018914 @default.

• next