FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158252425> ?p ?o ?g. }

• W2158252425 endingPage "F425" @default.
• W2158252425 startingPage "F421" @default.
• W2158252425 abstract "Superoxide (O(2)(-)) produced by NADPH oxidase regulates Na absorption and renal hemodynamics. Increased NaCl in the thick ascending limb (TAL) stimulates O(2)(-) generation. However, we do not know whether physiological changes in NaCl concentration augment O(2)(-) generation, nor do we know the mediator(s) involved. In other cells, Rac1, a regulatory subunit of NADPH oxidase, is activated by elevated NaCl. We hypothesized that increasing luminal NaCl within the physiological range activates Rac1 and NADPH oxidase and, thereby, increases O(2)(-) production. We increased NaCl from 10 to 57 mM in medullary TAL suspensions and used lucigenin to measure O(2)(-) generation and Western blot to measure Rac1 activity. Increasing NaCl stimulated O(2)(-) generation from 1.41 +/- 0.16 to 2.71 +/- 0.30 nmol O(2)(-) x min(-1) x mg protein(-1) (n = 6, P < 0.05). This increase was blocked by the Na-K-2Cl cotransporter inhibitor furosemide and the NADPH oxidase inhibitor apocynin. To examine the role of Rac1 in NaCl-induced O(2)(-) production, we measured Rac1 translocation by Western blot. When we added NaCl, Rac1 in the particulate fraction increased from 6.8 +/- 0.8 to 11.7 +/- 2.4% of total Rac1 (n = 7, P < 0.05). Then we measured O(2)(-) generation in the presence and absence of the Rac1 inhibitor. In the absence of the Rac1 inhibitor, NaCl increased O(2)(-) generation from 1.07 +/- 0.24 to 2.02 +/- 0.49 nmol O(2)(-) x min(-1) x mg protein(-1), and this increase was completely blocked by the inhibitor. Similarly, in vivo treatment of TALs with adenovirus expressing dominant-negative Rac1 decreased NaCl-induced O(2)(-) generation by 60% compared with control (0.33 +/- 0.04 vs. 0.81 +/- 0.17 nmol O(2)(-) x min(-1) x mg protein(-1), n = 6, P < 0.05). We concluded that physiological increases in NaCl stimulate TAL O(2)(-) generation by activating Rac1." @default.
• W2158252425 created "2016-06-24" @default.
• W2158252425 creator A5021025572 @default.
• W2158252425 creator A5073214978 @default.
• W2158252425 date "2010-02-01" @default.
• W2158252425 modified "2023-10-13" @default.
• W2158252425 title "Rac1 mediates NaCl-induced superoxide generation in the thick ascending limb" @default.
• W2158252425 cites W1981755821 @default.
• W2158252425 cites W1988186319 @default.
• W2158252425 cites W1998381684 @default.
• W2158252425 cites W2012487082 @default.
• W2158252425 cites W2018652427 @default.
• W2158252425 cites W2027052842 @default.
• W2158252425 cites W2033759877 @default.
• W2158252425 cites W2090749314 @default.
• W2158252425 cites W2096869450 @default.
• W2158252425 cites W2099386577 @default.
• W2158252425 cites W2102241641 @default.
• W2158252425 cites W2106913297 @default.
• W2158252425 cites W2109106144 @default.
• W2158252425 cites W2112913092 @default.
• W2158252425 cites W2127488207 @default.
• W2158252425 cites W2160108032 @default.
• W2158252425 cites W2164757969 @default.
• W2158252425 cites W2165441485 @default.
• W2158252425 cites W2204074942 @default.
• W2158252425 cites W2226433290 @default.
• W2158252425 cites W2411558902 @default.
• W2158252425 cites W2434260882 @default.
• W2158252425 cites W2468459478 @default.
• W2158252425 cites W3205509167 @default.
• W2158252425 doi "https://doi.org/10.1152/ajprenal.00472.2009" @default.
• W2158252425 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2822510" @default.
• W2158252425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19923407" @default.
• W2158252425 hasPublicationYear "2010" @default.
• W2158252425 type Work @default.
• W2158252425 sameAs 2158252425 @default.
• W2158252425 citedByCount "31" @default.
• W2158252425 countsByYear W21582524252012 @default.
• W2158252425 countsByYear W21582524252013 @default.
• W2158252425 countsByYear W21582524252016 @default.
• W2158252425 countsByYear W21582524252017 @default.
• W2158252425 countsByYear W21582524252019 @default.
• W2158252425 countsByYear W21582524252020 @default.
• W2158252425 countsByYear W21582524252021 @default.
• W2158252425 countsByYear W21582524252022 @default.
• W2158252425 crossrefType "journal-article" @default.
• W2158252425 hasAuthorship W2158252425A5021025572 @default.
• W2158252425 hasAuthorship W2158252425A5073214978 @default.
• W2158252425 hasBestOaLocation W21582524252 @default.
• W2158252425 hasConcept C104317684 @default.
• W2158252425 hasConcept C12554922 @default.
• W2158252425 hasConcept C153911025 @default.
• W2158252425 hasConcept C181199279 @default.
• W2158252425 hasConcept C185592680 @default.
• W2158252425 hasConcept C2776415932 @default.
• W2158252425 hasConcept C2777209548 @default.
• W2158252425 hasConcept C2779717556 @default.
• W2158252425 hasConcept C2779719074 @default.
• W2158252425 hasConcept C2780795997 @default.
• W2158252425 hasConcept C38485361 @default.
• W2158252425 hasConcept C48349386 @default.
• W2158252425 hasConcept C55493867 @default.
• W2158252425 hasConcept C62478195 @default.
• W2158252425 hasConcept C86803240 @default.
• W2158252425 hasConceptScore W2158252425C104317684 @default.
• W2158252425 hasConceptScore W2158252425C12554922 @default.
• W2158252425 hasConceptScore W2158252425C153911025 @default.
• W2158252425 hasConceptScore W2158252425C181199279 @default.
• W2158252425 hasConceptScore W2158252425C185592680 @default.
• W2158252425 hasConceptScore W2158252425C2776415932 @default.
• W2158252425 hasConceptScore W2158252425C2777209548 @default.
• W2158252425 hasConceptScore W2158252425C2779717556 @default.
• W2158252425 hasConceptScore W2158252425C2779719074 @default.
• W2158252425 hasConceptScore W2158252425C2780795997 @default.
• W2158252425 hasConceptScore W2158252425C38485361 @default.
• W2158252425 hasConceptScore W2158252425C48349386 @default.
• W2158252425 hasConceptScore W2158252425C55493867 @default.
• W2158252425 hasConceptScore W2158252425C62478195 @default.
• W2158252425 hasConceptScore W2158252425C86803240 @default.
• W2158252425 hasIssue "2" @default.
• W2158252425 hasLocation W21582524251 @default.
• W2158252425 hasLocation W21582524252 @default.
• W2158252425 hasLocation W21582524253 @default.
• W2158252425 hasLocation W21582524254 @default.
• W2158252425 hasOpenAccess W2158252425 @default.
• W2158252425 hasPrimaryLocation W21582524251 @default.
• W2158252425 hasRelatedWork W1968318107 @default.
• W2158252425 hasRelatedWork W1973953924 @default.
• W2158252425 hasRelatedWork W1989835627 @default.
• W2158252425 hasRelatedWork W2052191948 @default.
• W2158252425 hasRelatedWork W2093200537 @default.
• W2158252425 hasRelatedWork W2137518469 @default.
• W2158252425 hasRelatedWork W2165953544 @default.
• W2158252425 hasRelatedWork W2284446819 @default.
• W2158252425 hasRelatedWork W801223904 @default.
• W2158252425 hasRelatedWork W2102242603 @default.
• W2158252425 hasVolume "298" @default.

• next