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• W2164201963 abstract "Five different mechanical cell disruption processes were evaluated as methods to extract plasmids from bacterial cells. The methods used were sonication, nebulization homogenization, microfluidization, and bead milling. The recovery yields of intact plasmids from the various methods were measured by quantitative gel electrophoresis. Bead milling and microfluidization were found to have the highest potential for large scale extraction with total intact recoveries of over 90% and around 50%, respectively. Other methods resulted in substantial plasmid degradation, with recoveries no greater than 20% of the total intact plasmid. © 1995 John Wiley & Sons, Inc." @default.
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• W2164201963 date "1995-11-20" @default.
• W2164201963 modified "2023-10-17" @default.
• W2164201963 title "Mechanical disruption ofEscherichia coli for plasmid recovery" @default.
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• W2164201963 doi "https://doi.org/10.1002/bit.260480403" @default.
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